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The map label for this gene is dcuB [H]

Identifier: 148825579

GI number: 148825579

Start: 494997

End: 496328

Strand: Direct

Name: dcuB [H]

Synonym: CGSHiEE_02520

Alternate gene names: 148825579

Gene position: 494997-496328 (Clockwise)

Preceding gene: 148825578

Following gene: 148825580

Centisome position: 27.3

GC content: 39.71

Gene sequence:

>1332_bases
ATGCTTTATTTAGAATTTTTATTTTTACTATTAATGCTCTATATCGGTAGCCGTTACGGCGGTATCGGATTAGGTGTTGT
TTCTGGTATCGGTCTTGCTATCGAGGTTTTCGTATTTCGTATGCCAGTAGGGAAAGCACCGATTGATGTTATGCTTATCA
TTCTTGCAGTGGTGACTTGCGCATCCGTACTTGAAGCAGCTGGCGGTTTGAAATTTATGTTACAAATTGCTGAACGCATT
CTCCGTAAAAATCCAAAACGTGTCACTTTACTCGGCCCATTAGTCGTCTATTTATTAACCTTTATGCTTGGTACTGGTCA
CTCTGTTTACACAATTATGCCAATCATCGGCGATGTTGCATTGAAAAACAAAATTCGTCCAGAACGTCCAATGGCAGCGT
CTTCGGTAGCATCGCAATTAGCCATCACATCAAGCCCACTTTCTGCAGCTGTTGTATTTTATCTAGGTAAAATTACAGCA
ATGCCCGCTTTTGAGCATATTAGTTTATTAGATATTATTTGTGTAACCGTTCCTGCTACTTTAGCGGGTACGATTGCGCT
TTCTTTATACAGTATGCGTCGTGGTAAAGAGTTAGAACAAGATCCTGAATATCAACGTCGTTTACAAGATCCAGTATGGC
GCGAACGTATTTTAAATACTACCAGTACAACATTGAATGAAACCCTTCCGCAAGGGGCAAAAAAATCTGTTTACCTATTT
TTACTTGCTTTAGTTGTGATTGTTGCGATTGCGATGATTCCTGAAATTCGCACTATCGGTAGCGGAAAAGCAATTTCAAT
GTCACTTATCATTCAAATGATGATGCTTTGCTTTGGCGGAGTGATTTTACTTGCAACGAAAACAAATCCTCAAATCGTGC
CAAATGGCGTGGTATTTAAGTCAGGTATGGTAGCAGCGATTGCAATTTATGGCATTGCGTGGATGAGCGACACTTACTTC
AAATATGCAATACCTGAATTTAAAGCTGCGATTACTGATATGGTGCAAACTTACCCTTGGACATTCGCATTGGCGTTATT
TGCAGTCTCTGTTGTAATTAATAGCCAAGCTGCCACTGCTGTAATGTTATTACCTGTGGGTATAAGTTTAGGTATTCCTG
CGCCAATCTTAGTTGGTTTAATGCCTGCAACTTATGCGTATTTCTTCATTCCAAATTATCCGTCAGATATTGCAACAGTA
AACTTTGACGTAACGGGTACAACAAAAATTGGTAAATATTATTTTAACCACAGTTTCATGGTGCCAGGTTTAATTGGGGT
TGTTGTTGCTTGCTTGGTCGGTGTTTCTGTGGCGGAATTAGTGATTCGCTAA

Upstream 100 bases:

>100_bases
TATCTTTTACTTAAAATCTTGGTTTAAATTTTAAAAAATTATTTAGTTATATGCCTAAAAAGAAGTATTATTTGCATAGT
TTTTCATTTTTGAGGTGTTT

Downstream 100 bases:

>100_bases
TTTACACACAAACTAAATCCTTTTAATATAAAAGCACCTTTGAATGGTGCTTTTATTTTATGACAACCTACATCGCCTAC
GGCAATATAAATCAACCTTT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLYLEFLFLLLMLYIGSRYGGIGLGVVSGIGLAIEVFVFRMPVGKAPIDVMLIILAVVTCASVLEAAGGLKFMLQIAERI
LRKNPKRVTLLGPLVVYLLTFMLGTGHSVYTIMPIIGDVALKNKIRPERPMAASSVASQLAITSSPLSAAVVFYLGKITA
MPAFEHISLLDIICVTVPATLAGTIALSLYSMRRGKELEQDPEYQRRLQDPVWRERILNTTSTTLNETLPQGAKKSVYLF
LLALVVIVAIAMIPEIRTIGSGKAISMSLIIQMMMLCFGGVILLATKTNPQIVPNGVVFKSGMVAAIAIYGIAWMSDTYF
KYAIPEFKAAITDMVQTYPWTFALALFAVSVVINSQAATAVMLLPVGISLGIPAPILVGLMPATYAYFFIPNYPSDIATV
NFDVTGTTKIGKYYFNHSFMVPGLIGVVVACLVGVSVAELVIR

Sequences:

>Translated_443_residues
MLYLEFLFLLLMLYIGSRYGGIGLGVVSGIGLAIEVFVFRMPVGKAPIDVMLIILAVVTCASVLEAAGGLKFMLQIAERI
LRKNPKRVTLLGPLVVYLLTFMLGTGHSVYTIMPIIGDVALKNKIRPERPMAASSVASQLAITSSPLSAAVVFYLGKITA
MPAFEHISLLDIICVTVPATLAGTIALSLYSMRRGKELEQDPEYQRRLQDPVWRERILNTTSTTLNETLPQGAKKSVYLF
LLALVVIVAIAMIPEIRTIGSGKAISMSLIIQMMMLCFGGVILLATKTNPQIVPNGVVFKSGMVAAIAIYGIAWMSDTYF
KYAIPEFKAAITDMVQTYPWTFALALFAVSVVINSQAATAVMLLPVGISLGIPAPILVGLMPATYAYFFIPNYPSDIATV
NFDVTGTTKIGKYYFNHSFMVPGLIGVVVACLVGVSVAELVIR
>Mature_443_residues
MLYLEFLFLLLMLYIGSRYGGIGLGVVSGIGLAIEVFVFRMPVGKAPIDVMLIILAVVTCASVLEAAGGLKFMLQIAERI
LRKNPKRVTLLGPLVVYLLTFMLGTGHSVYTIMPIIGDVALKNKIRPERPMAASSVASQLAITSSPLSAAVVFYLGKITA
MPAFEHISLLDIICVTVPATLAGTIALSLYSMRRGKELEQDPEYQRRLQDPVWRERILNTTSTTLNETLPQGAKKSVYLF
LLALVVIVAIAMIPEIRTIGSGKAISMSLIIQMMMLCFGGVILLATKTNPQIVPNGVVFKSGMVAAIAIYGIAWMSDTYF
KYAIPEFKAAITDMVQTYPWTFALALFAVSVVINSQAATAVMLLPVGISLGIPAPILVGLMPATYAYFFIPNYPSDIATV
NFDVTGTTKIGKYYFNHSFMVPGLIGVVVACLVGVSVAELVIR

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=403, Percent_Identity=44.9131513647643, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1790580, Length=437, Percent_Identity=35.4691075514874, Blast_Score=244, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47894; Mature: 47894

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLEFLFLLLMLYIGSRYGGIGLGVVSGIGLAIEVFVFRMPVGKAPIDVMLIILAVVTC
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASVLEAAGGLKFMLQIAERILRKNPKRVTLLGPLVVYLLTFMLGTGHSVYTIMPIIGDVA
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
LKNKIRPERPMAASSVASQLAITSSPLSAAVVFYLGKITAMPAFEHISLLDIICVTVPAT
HHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAGTIALSLYSMRRGKELEQDPEYQRRLQDPVWRERILNTTSTTLNETLPQGAKKSVYLF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHH
LLALVVIVAIAMIPEIRTIGSGKAISMSLIIQMMMLCFGGVILLATKTNPQIVPNGVVFK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEC
SGMVAAIAIYGIAWMSDTYFKYAIPEFKAAITDMVQTYPWTFALALFAVSVVINSQAATA
CCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHE
VMLLPVGISLGIPAPILVGLMPATYAYFFIPNYPSDIATVNFDVTGTTKIGKYYFNHSFM
EEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCH
VPGLIGVVVACLVGVSVAELVIR
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLYLEFLFLLLMLYIGSRYGGIGLGVVSGIGLAIEVFVFRMPVGKAPIDVMLIILAVVTC
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASVLEAAGGLKFMLQIAERILRKNPKRVTLLGPLVVYLLTFMLGTGHSVYTIMPIIGDVA
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
LKNKIRPERPMAASSVASQLAITSSPLSAAVVFYLGKITAMPAFEHISLLDIICVTVPAT
HHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAGTIALSLYSMRRGKELEQDPEYQRRLQDPVWRERILNTTSTTLNETLPQGAKKSVYLF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHH
LLALVVIVAIAMIPEIRTIGSGKAISMSLIIQMMMLCFGGVILLATKTNPQIVPNGVVFK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEC
SGMVAAIAIYGIAWMSDTYFKYAIPEFKAAITDMVQTYPWTFALALFAVSVVINSQAATA
CCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHE
VMLLPVGISLGIPAPILVGLMPATYAYFFIPNYPSDIATVNFDVTGTTKIGKYYFNHSFM
EEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCH
VPGLIGVVVACLVGVSVAELVIR
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]