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The map label for this gene is pflA [C]

Identifier: 148825569

GI number: 148825569

Start: 486819

End: 487418

Strand: Direct

Name: pflA [C]

Synonym: CGSHiEE_02470

Alternate gene names: 148825569

Gene position: 486819-487418 (Clockwise)

Preceding gene: 148825567

Following gene: 148825570

Centisome position: 26.85

GC content: 35.5

Gene sequence:

>600_bases
ATGACATTAGTAAACAAAATTAAAACATTATCATCAGTAGGTATTCTAGCGGCTACATTATTTCTTGCAGGCTGCCAAGC
ACAATCAAATATATTAGCATTTACACCGCCTGCGCCAAGTGCTTCAATGAATGTTAATCGAACTGCCGTTGTATCTGTTA
CAACAAAAGATAGCCGTGCAATACAAGAGATTGCGAGTTATACGAAACACGGGGAACTGATTAAATTAAATGCATCCCCA
AGTGTTACACAATTATTTCAGCAAGTGATGCAGCAAAATTTAATTAGTAAAGGCTTTAGAATTGGGCAATTAAATGGTTC
AAATGCGTGGGTAACTGTGGATGTGCGTGAATTTGGTACGCAAGTAGAACAAGGTAATCTTCGTTATAAACTTAATACCA
AAATTCAAGCGACAGTTTATGTACAAGGTGCGAAAGGTTCGTATAATAAATCATTTAATGTCACGCGTTCACAAGAGGGC
GTATTTAATGCGGACAATGATGAAATTCATAAAGTGCTATCTCAAACTTTTAATGATATTGTGAACAATATTTATCAAGA
TCAAGAAGTTGCGGCTGCGATAAACCAATATTCTAATTAA

Upstream 100 bases:

>100_bases
TTGACATTATAAGTTCTCCACAAAAACTACAATGAAATGCTATACATTAATAAGGAAGTTTGTAAGAATAGCACAATTCT
AATTGATTTATGGGGAAACT

Downstream 100 bases:

>100_bases
TCACATATCTACAGTGAGGAAGATTGAACAATCTTCCTCTTTTTCTATCACGCTATTTCATTCTACATACTAGGCTTAAC
ATAAAATCATTTCAAACTAA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTLVNKIKTLSSVGILAATLFLAGCQAQSNILAFTPPAPSASMNVNRTAVVSVTTKDSRAIQEIASYTKHGELIKLNASP
SVTQLFQQVMQQNLISKGFRIGQLNGSNAWVTVDVREFGTQVEQGNLRYKLNTKIQATVYVQGAKGSYNKSFNVTRSQEG
VFNADNDEIHKVLSQTFNDIVNNIYQDQEVAAAINQYSN

Sequences:

>Translated_199_residues
MTLVNKIKTLSSVGILAATLFLAGCQAQSNILAFTPPAPSASMNVNRTAVVSVTTKDSRAIQEIASYTKHGELIKLNASP
SVTQLFQQVMQQNLISKGFRIGQLNGSNAWVTVDVREFGTQVEQGNLRYKLNTKIQATVYVQGAKGSYNKSFNVTRSQEG
VFNADNDEIHKVLSQTFNDIVNNIYQDQEVAAAINQYSN
>Mature_198_residues
TLVNKIKTLSSVGILAATLFLAGCQAQSNILAFTPPAPSASMNVNRTAVVSVTTKDSRAIQEIASYTKHGELIKLNASPS
VTQLFQQVMQQNLISKGFRIGQLNGSNAWVTVDVREFGTQVEQGNLRYKLNTKIQATVYVQGAKGSYNKSFNVTRSQEGV
FNADNDEIHKVLSQTFNDIVNNIYQDQEVAAAINQYSN

Specific function: Unknown

COG id: COG3056

COG function: function code M; Uncharacterized lipoprotein

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli yajG [H]

Homologues:

Organism=Escherichia coli, GI87081739, Length=187, Percent_Identity=26.2032085561497, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005619 [H]

Pfam domain/function: PF03923 Lipoprotein_16 [H]

EC number: NA

Molecular weight: Translated: 21779; Mature: 21648

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVNKIKTLSSVGILAATLFLAGCQAQSNILAFTPPAPSASMNVNRTAVVSVTTKDSRA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCHHH
IQEIASYTKHGELIKLNASPSVTQLFQQVMQQNLISKGFRIGQLNGSNAWVTVDVREFGT
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHCC
QVEQGNLRYKLNTKIQATVYVQGAKGSYNKSFNVTRSQEGVFNADNDEIHKVLSQTFNDI
EECCCCEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VNNIYQDQEVAAAINQYSN
HHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure 
TLVNKIKTLSSVGILAATLFLAGCQAQSNILAFTPPAPSASMNVNRTAVVSVTTKDSRA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCHHH
IQEIASYTKHGELIKLNASPSVTQLFQQVMQQNLISKGFRIGQLNGSNAWVTVDVREFGT
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHCC
QVEQGNLRYKLNTKIQATVYVQGAKGSYNKSFNVTRSQEGVFNADNDEIHKVLSQTFNDI
EECCCCEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VNNIYQDQEVAAAINQYSN
HHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]