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The map label for this gene is nagK [H]

Identifier: 148825565

GI number: 148825565

Start: 483065

End: 483979

Strand: Direct

Name: nagK [H]

Synonym: CGSHiEE_02450

Alternate gene names: 148825565

Gene position: 483065-483979 (Clockwise)

Preceding gene: 148825562

Following gene: 148825566

Centisome position: 26.64

GC content: 40.87

Gene sequence:

>915_bases
ATGTATTATGGTTTAGATATTGGTGGCACGAAAATAGAACTTGCCGTATTCAATGAAAAATTAGAAAAATTATATTCTGA
ACGGGTGCCAACACCTAAAACCGACTATGAAGAATGGCTTAACACGATTGTGGATTTAGTGAATCGTGCTGATGAAAAGT
TTGGTGAAGTGGGCACCGTAGGTTTGGGCGTACCTGGTTTTGTTAATCAACAAACTGGATTAGCCGAAATTACCAATATT
CGTGTGGCAGATAATAAACCGATTTTACGCGATTTGTCTGCACGTTTAGGCCGTGAAGTGCGGGCAGAAAATGACGCAAA
TTGTTTCGCGTTATCTGAAGCATGGGATGCTGAAAATCAGCAATATCCGACTGTACTTGGTTTAATTCTTGGAACTGGTT
TTGGTGGTGGCTTTGTCTTAAATGGTAAAGTTCATTCAGGTCAAGTGGGAATGGCGGGAGAGCTTGGTCATTTACAACTA
AATTATCATGCCTTGAAATTATTGGGATGGGATAAAGCCCCAATTTATCAATGCGGTTGTGGCAACAAGGCTTGTTTGGA
TAATTATTTGTCTGGTCGCGGTTTTGAAATGCTTTATCGAGATTTGAAAGGCGAAACATTATCCGCTCGTGAAATTATTG
ATTTGTTCTATCAAGGCAATGAAAGTGCGGTAGATTTTGTGAACCTTTTTGTTGAGTTGGCGGCGATTTCTATTGGCAAT
ATCATTACGGCATTTGATCCGCATATGATTGTGCTAGGCGGTGGATTATCTAATTTTGATTACCTTTATGAGGCTTTACC
AAAAGCACTACCGCCTCACTTGATGCGGACAGCAAAAGTTCCGCCAATTAAAAAAGCGAAACACGGCGATTCTGGTGGTG
TGCGTGGCGCGGCAGCGTTGTTTTTAACCAAATAA

Upstream 100 bases:

>100_bases
TCTTTTTGAAGAAAAAGGCGATCTTTGTCATAAAATAATGATTTAAGTGCGGTTAAAATTTGGTTAATTTTAAGTGCACT
TCTTTATACAGGAGCAGATT

Downstream 100 bases:

>100_bases
TTCAACAAACTAGTGGGGCGTATTCAAATAATATGCCCTTTGTTTTTGAAAAATATTTAGAATATTCTAAATTTATTAGT
ATTTTTTCTTTTTTGTTTTT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEITNI
RVADNKPILRDLSARLGREVRAENDANCFALSEAWDAENQQYPTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDKAPIYQCGCGNKACLDNYLSGRGFEMLYRDLKGETLSAREIIDLFYQGNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRTAKVPPIKKAKHGDSGGVRGAAALFLTK

Sequences:

>Translated_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEITNI
RVADNKPILRDLSARLGREVRAENDANCFALSEAWDAENQQYPTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDKAPIYQCGCGNKACLDNYLSGRGFEMLYRDLKGETLSAREIIDLFYQGNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRTAKVPPIKKAKHGDSGGVRGAAALFLTK
>Mature_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEITNI
RVADNKPILRDLSARLGREVRAENDANCFALSEAWDAENQQYPTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDKAPIYQCGCGNKACLDNYLSGRGFEMLYRDLKGETLSAREIIDLFYQGNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRTAKVPPIKKAKHGDSGGVRGAAALFLTK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=50.4950495049505, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=41.0596026490066, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1790522, Length=314, Percent_Identity=27.3885350318471, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786891, Length=271, Percent_Identity=22.5092250922509, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 33127; Mature: 33127

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01125 ROK ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GLGVPGFVNQQTGLAEITNIRVADNKPILRDLSARLGREVRAENDANCFALSEAWDAENQ
CCCCCCCCCCCCCCEEHEEEEECCCCCHHHHHHHHHCCHHCCCCCCCEEEECCCCCCCCC
QYPTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDKAPIYQCGC
CCHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCC
GNKACLDNYLSGRGFEMLYRDLKGETLSAREIIDLFYQGNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRTAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC
>Mature Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GLGVPGFVNQQTGLAEITNIRVADNKPILRDLSARLGREVRAENDANCFALSEAWDAENQ
CCCCCCCCCCCCCCEEHEEEEECCCCCHHHHHHHHHCCHHCCCCCCCEEEECCCCCCCCC
QYPTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDKAPIYQCGC
CCHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCC
GNKACLDNYLSGRGFEMLYRDLKGETLSAREIIDLFYQGNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRTAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA