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The map label for this gene is birA [H]

Identifier: 148825496

GI number: 148825496

Start: 375138

End: 376043

Strand: Direct

Name: birA [H]

Synonym: CGSHiEE_01975

Alternate gene names: 148825496

Gene position: 375138-376043 (Clockwise)

Preceding gene: 148825493

Following gene: 148825497

Centisome position: 20.69

GC content: 34.99

Gene sequence:

>906_bases
ATGAATTTTACGTTATTGACATATTTATCGGATTGTCAGACAAAAGTGCGGTCTGAATTGGAAAAGTTTTCTAAAAATCT
AGAGGAAGATATTCAACCATTACGTGAAATTGGTTTGGATATTCTCGTTGATGGACAACATTATCGCTTAGTGCCAATGC
TTCCTTTATTAAATCCTCAGCAAATATCAACCGCACTTTTTCCTTATGGCATCCATTATCAGCCGATAATTTCCTCTACA
AATGAATGGATACTACAAAATATTCTTTCATTAAAAAAAGGCGATCTTTGTGTGGCTGAATATCAAACTGCAGGGCGCGG
TCGGCGTGGTCGCCAATGGTTATCGCCTTTTGCTGGGCAAATCATGTTTAGTTTTTATTGGGCATTTGATCCTAAAAAAT
CAATTGAGGGGTTAAGTTTAGTCATCGGTTTGGCGATTGCGGAAGTGCTAAATGTACAAGTGAAATGGCCAAATGATATT
TTGTTTGATGAAAGAAAGTTAGGTGGCATTTTAGTTGAAATTGCTAATCATAAAAATGGCATGCTCAATTTAGTGATTGG
CATAGGTATTAATGTGTCGTTGTCAAAACAAACAGAAATTAGTCAGCCTTATGCGGAAGTGTGTGAAATTGATCCTGATG
TAGAGCGACAAACTTTATTACCCAAACTTATACAACATTTATATACACGTTTAAATATCTTTGAGCAAAATGGTATTGAT
GAGGAATTTCAACAAGCATGGCAATCATATAATGCGTTTTCAAATAGTGAAGTAAAGGTGCTTACTGAGCAAGGTGTTAT
TTCAGGTATTGAACAAGGCATAGATGAACGTGGTTATTTAAAAGTATTATGTGGCAATAAAATTCAGATGTTTAATGGTG
GAGAAGTTTCATTACGTAAGAAATAA

Upstream 100 bases:

>100_bases
AAATATTGCGGCGGGATTATACAGATTTTACTTGATTTTGAAAACGAAATTTTTGGATTTATGCTATGATTTTCATACTA
TTTGCCATAAGGAATGAATG

Downstream 100 bases:

>100_bases
TAGCAATAAAAGTTTGACTATTCTAACCGCACTTTTTAATAAAATGTTTATATTTCCATCAAAAGATCATTTTTTTAAAT
ATTTTTGCAAAAAGTACTTG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNFTLLTYLSDCQTKVRSELEKFSKNLEEDIQPLREIGLDILVDGQHYRLVPMLPLLNPQQISTALFPYGIHYQPIISST
NEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDI
LFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGID
EEFQQAWQSYNAFSNSEVKVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK

Sequences:

>Translated_301_residues
MNFTLLTYLSDCQTKVRSELEKFSKNLEEDIQPLREIGLDILVDGQHYRLVPMLPLLNPQQISTALFPYGIHYQPIISST
NEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDI
LFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGID
EEFQQAWQSYNAFSNSEVKVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK
>Mature_301_residues
MNFTLLTYLSDCQTKVRSELEKFSKNLEEDIQPLREIGLDILVDGQHYRLVPMLPLLNPQQISTALFPYGIHYQPIISST
NEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDI
LFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGID
EEFQQAWQSYNAFSNSEVKVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=284, Percent_Identity=40.1408450704225, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI212645369, Length=225, Percent_Identity=28, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32563944, Length=218, Percent_Identity=28.4403669724771, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32563942, Length=218, Percent_Identity=28.4403669724771, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34192; Mature: 34192

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTLLTYLSDCQTKVRSELEKFSKNLEEDIQPLREIGLDILVDGQHYRLVPMLPLLNPQ
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCHH
QISTALFPYGIHYQPIISSTNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQ
HHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHH
IMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDILFDERKLGGILVEIANHKNG
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHCEEEEEEECCCCC
MLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGID
EEEEEEEECCEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEFQQAWQSYNAFSNSEVKVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRK
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCEEEEC
K
C
>Mature Secondary Structure
MNFTLLTYLSDCQTKVRSELEKFSKNLEEDIQPLREIGLDILVDGQHYRLVPMLPLLNPQ
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCHH
QISTALFPYGIHYQPIISSTNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQ
HHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHH
IMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDILFDERKLGGILVEIANHKNG
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHCEEEEEEECCCCC
MLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGID
EEEEEEEECCEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEFQQAWQSYNAFSNSEVKVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRK
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]