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The map label for this gene is aspS [H]

Identifier: 148825398

GI number: 148825398

Start: 269647

End: 271413

Strand: Direct

Name: aspS [H]

Synonym: CGSHiEE_01440

Alternate gene names: 148825398

Gene position: 269647-271413 (Clockwise)

Preceding gene: 148825390

Following gene: 148825399

Centisome position: 14.87

GC content: 41.71

Gene sequence:

>1767_bases
ATGATGCGTACACATTATTGCGGAGCATTAAACCGTAACAATATCGGACAAGATGTAACATTAAGCGGTTGGGTTCACCG
CCGTCGTGATTTAGGTGGCTTGATTTTTATTGATATGCGTGATCGTGATGGCATTGTGCAAGTTTGTTTTGATCCGAAAT
ATCAAGATGCATTGACAGCGGCTGCTGGGTTACGTAATGAATTTTGTATTCAAATTAAAGGTGAAGTGATTGCGCGTCCT
GAAAACCAAATCAATAAAAATATGGCAACAGGTGAAGTGGAAGTGTTAGCGAAAGAATTGCGTGTTTACAATGCTTCTGA
TGTGTTGCCTCTCGACTTTAACCAAAACAATACTGAAGAACAACGTTTAAAATATCGTTATTTAGATTTACGTCGTCCAG
AAATGGCTCAACGTTTGAAAACTCGTGCAAAAATCACCAGCTTTGTGCGTCGTTTCATGGATGATAATGGTTTCCTTGAT
ATTGAAACCCCAATGCTTACCAAAGCAACGCCAGAAGGTGCGCGTGACTATTTAGTGCCAAGCCGTGTGCATAAAGGCAA
ATTCTATGCATTGCCGCAATCACCACAGCTTTTCAAACAGCTTTTAATGATGTCTGGTTTTGACCGCTATTATCAAATCG
TAAAATGTTTCCGTGATGAAGATTTGCGAGCAGACCGTCAGCCTGAGTTTACTCAAATCGATGTGGAAACTTCTTTCCTA
ACTGCGCCAGAAGTCCGCGAAATTATGGAACGTATGGTGCACGGCTTATGGCTTGATACCATCGGTGTGGATTTAGGTAA
GTTCCCAGTGATGACTTGGCAAGAAGCAATGCGTCGTTTTGGTTCGGATAAGCCAGATTTGCGTAATCCATTAGAAATAG
TAGATGTAGCAGATATTGTCAAAGATGTTGAATTTAAAGTATTTAATGAGCCAGCAAACAATCCAAATGGCCGTGTTGCA
GTCATTCGAGTACCAAATGGTGCAGAAATTACTCGTAAACAAATTGATGAATATACACAATTTGTAGGCATCTACGGTGC
AAAAGGTTTGGCTTGGGCAAAAGTAAACGATATTAATGCTGGTCTTGAAGGCGTGCAAAGTCCGATTGCGAAATTCTTAA
ATGAAGAGGTATGGAAAGCGTTAGCAGAACGTGTGAATGCTCAAACTGGCGATATTTTATTCTTCGGTGCAGACAAATGG
CAAACGACCACTGATGCAATGGGCGCGTTACGTTTGAAATTAGGTCGTGATCTTGGCTTAACTCGTTTAGACGAATGGAA
ACCGCTTTGGGTCATTGATTTCCCAATGTTTGAACGTGATGAAGAAGGTAATCTTGCAGCAATGCACCATCCATTCACTT
CACCAAAAGATTTCAGCCCAGAGCAATTAGAGGCTGATCCAACAAGTGCGGTAGCCAATGCTTACGATATGGTCATCAAC
GGCTACGAAGTGGGTGGTGGTTCTGTACGTATTTTTGATCCGAAAATGCAACAAACTGTGTTCCGTATTCTTGGTATTGA
CGAAGAACAACAACGCGAGAAATTCGGTTTCTTATTAGATGCGTTAAAATTTGGTACACCGCCACACGCAGGTCTTGCAT
TCGGTTTAGACCGCTTAACCATGCTTTTAACAGGCACGGAAAACATTCGCGATGTAATTGCTTTTCCTAAAACTACGGCA
GCAGCGTGCTTAATGACAGAAGCACCAAGTTTTGCGAATCCGCAGGCATTGGAAGAGTTAGCGATTCAAGTTACTAAGTC
TGAATAA

Upstream 100 bases:

>100_bases
TTGATTTGTCAGCATAAATAAACTTTTTATCTCTCTTGGTTTTCCGCTATAATTTCGACAGATTTTTGAGCATAATTAAA
AGAGTACAAAGGATTTTGAA

Downstream 100 bases:

>100_bases
TGTAAGGGGGATCATTATTCCCCTTTTGTTTTATCTATAATTAGCGATTAAGGAATTTACTATGAAAAGTATTCTTAAAT
TTTTATTATTTTTCGGAGTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRVYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEIVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWKPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAIQVTKSE

Sequences:

>Translated_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRVYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEIVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWKPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAIQVTKSE
>Mature_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRVYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEIVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWKPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAIQVTKSE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=37.9139072847682, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=29.2490118577075, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=29.2490118577075, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI45439306, Length=253, Percent_Identity=25.296442687747, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI226491502, Length=271, Percent_Identity=23.6162361623616, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=76.4102564102564, Blast_Score=930, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=290, Percent_Identity=28.6206896551724, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=27.3356401384083, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=34.2237061769616, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=248, Percent_Identity=24.1935483870968, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=263, Percent_Identity=23.574144486692, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=336, Percent_Identity=27.6785714285714, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=634, Percent_Identity=35.0157728706625, Blast_Score=300, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=26.4, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=259, Percent_Identity=25.0965250965251, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=289, Percent_Identity=25.2595155709343, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584738, Length=591, Percent_Identity=37.7326565143824, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24640851, Length=264, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=26.1410788381743, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66709; Mature: 66709

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AAGLRNEFCIQIKGEVIARPENQINKNMATGEVEVLAKELRVYNASDVLPLDFNQNNTEE
HHCCCCCEEEEECCCEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEIVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
GLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWKPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAIQVTKSE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHEECCC
>Mature Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AAGLRNEFCIQIKGEVIARPENQINKNMATGEVEVLAKELRVYNASDVLPLDFNQNNTEE
HHCCCCCEEEEECCCEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEIVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
GLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWKPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAIQVTKSE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA