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The map label for this gene is priA [H]

Identifier: 148825377

GI number: 148825377

Start: 245622

End: 247814

Strand: Direct

Name: priA [H]

Synonym: CGSHiEE_01305

Alternate gene names: 148825377

Gene position: 245622-247814 (Clockwise)

Preceding gene: 148825367

Following gene: 148825390

Centisome position: 13.55

GC content: 41.5

Gene sequence:

>2193_bases
ATGAAAATTGTCCGAGTGGTGCTGGCTGTGCCACTTCCTCGTTTATTTGATTATCTCGTCCCCGATGATGTTTCATTACA
AATCGGTATGCGTGTTCTTGTGCCTTTTGGCACGCAAAAACGTGTCGCCATTGTGGCTGATTTCCCGACAAAATCGGACG
TGCCAGAGGATAAGCTCAAAGCCATTCTTCAACCATTAGATTTAGCACCGCTCTTTACCCCAATTTATTGGGATTGGTTG
CATTGGGCAGCAAATTATTATCAAGCGGGATTAGGCGATGTGTTATTTCAAGCCTTGCCTGTGAAATTACGCAACGGCGA
AAGTGCGGTGAAAAATGACCGCACTTTTTGGCGAATTACGGATGCAGGGAAAAATGCCCTTGAACAAGGCGAATTAAAAC
GCTCGAAAAAGCAAGCTGAAGCCCTCCAATACTTATCTGAAACTGATCTTGAAAAAGGCAATAATAATTTTTCCTCTGCC
ATTTGGTCAGCCTTAAAAGCCAAAGGGTTTATTGAGGAAATTACTATCCAAACAAAGCCCCTAAGCTGGCAACAAAACTT
AGGCGATAACCCAATTGTCAATGCTGAAAATCGTTTAACCTTAAATAAGCAGCAAGCCTTAGCGTTTAGTCAATTGCGTT
TTCATTCTGGTTTCAATGTATGGCTGCTCGATGGGGTGACGGGTTCAGGCAAAACAGAAATTTATCTGCAGTATATTGAA
GAAATTTTAAAATCGGGTAAGCAAGTATTAGTGCTTGTGCCCGAAATCGGGCTAACACCTCAAACGGTGCACCGTTTTAA
AGCACGTTTTAATGTAGAAATTGATGTTCTTCATTCTAATTTAACCGATACACAACGGCTTTATGTATGGGATCGTGCCC
GCTCTGGACAAAGTGCCATTGTGATTGGCACGAGATCGGCATTGTTCACGCAATTTTCTAATCTTGGTGCAATTATTTTG
GATGAAGAACACGATACATCTTACAAACAGCAAGATAGTTGGCGTTACCATGCGCGAGATTTAGCAACCGTACTTGCTCA
AAAACTAAATATTGCGGTATTAATGGGCTCCGCCACGCCAAGTTTAGAAAGTATTAATAATGTACAAAACGGCAAATACC
AACATTTAGTTTTATCAAAAAGAGCGGGAAATTCTACCGCACTTCGTCATTTCGTTATTGATTTGAAAAATCAACACATA
CAAAACGGGCTTTCAAAACCCTTATTGGAACGAATGAAAGCTCACCTCGAAAAAGGCAATCAAGTGTTGCTTTTCTTAAA
TCGTCGAGGCTTTGCACCCGTATTGCTTTGCCATGAATGTGGTTGGATCGCACAATGCCCTCATTGTGAAAAACCTTATA
CCTATCATCAGCATCAAAATGTATTGCGTTGTCATCATTGTGGCGCTCAGAAAACTATTCCTCGTCAATGTGGCGATTGC
GGCTCGACTCATTTAGTCACCACAGGTCTCGGCACGGAACAATTAGAAGAAACCTTGAAAACGCTATTTCCTCATTATTC
TGTAGCTCGAATCGACCGCGATAGCACTGCGCGTAAAGGAAAATTGGAGAGTTATTTAGAAGATATTCAACAAGGCAAAA
GTCAAATTTTGATTGGCACACAAATGCTGGCGAAAGGACATCATTTTCCGAATGTTACCTTGGTTGCCTTAGTGAATGTA
GATAGTGCATTATTTTCGCTAGATTTTCGTGCAGAAGAACGTTTAGCTCAACTTTATATTCAAGTTGCTGGACGAGCTGG
ACGTGCCGATAAACAAGGCGAAGTGGTGCTACAAACACATTATCCAGATCATCCTTTGCTTACCACGTTATTAGCGAATG
GCTACCAAGCTTTTGCAAAAGAAACCTTACAATTACGCCATTCAATGGGATTGCCACCTTTCACTTTTCAGGCGTTATTT
AAAGCACAGGCTCGACATTCTGAGCTTGCACAAAATTGCTTAATCCAGATCGCAGATTTTTTTCAATCAAAGCAAATCAC
GGGATTGCAAATGCTTGGCCCTATGCCTGCACCGTTCAGCAAGAAAGCGGGGCAATATCGCTGGCAGTTATTATTGCAAC
ATCCATCAAGAATGACGTTGCAAAAAGCATTGAGAGAATATCAACAAGCCGAACTTGAAAAAAACAGCCAAGTGCGGTTA
ATTTTGGACGTCGATCCACAAGACTTATCATAA

Upstream 100 bases:

>100_bases
CAAAAGTTTGTGTCATAAAAATCTTGTTCGTGAAAAAAATTGGCGGCGATTATACCAATTTTTCTCGTATAATAGGGCAA
ATTTTTTCCCAAGGTTGATT

Downstream 100 bases:

>100_bases
AAAAATGCGGTGAAAATTCACCGCACTTTTATCTCTAAAATCTATTTGAAATCTTCGACATCTCCTAATAGACAGGCATA
TTTCGCCGTATTGGGGCTGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSA
IWSALKAKGFIEEITIQTKPLSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALF
KAQARHSELAQNCLIQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS

Sequences:

>Translated_730_residues
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSA
IWSALKAKGFIEEITIQTKPLSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALF
KAQARHSELAQNCLIQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS
>Mature_730_residues
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSA
IWSALKAKGFIEEITIQTKPLSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALF
KAQARHSELAQNCLIQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=737, Percent_Identity=51.831750339213, Blast_Score=752, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=211, Percent_Identity=25.1184834123223, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82424; Mature: 82424

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLK
CHHHHHHHHCCHHHHHHHHCCCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
AILQPLDLAPLFTPIYWDWLHWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRIT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCHHHCCCCEEEEEE
DAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSAIWSALKAKGFIEEITIQTKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEEEECCC
LSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
CCHHHCCCCCCEECCCCCEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAI
HHHHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCCCCEE
VIGTRSALFTQFSNLGAIILDEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATP
EEECHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCC
SLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHIQNGLSKPLLERMKAHLEKGN
CHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
QVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
EEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHHCCC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGT
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEHH
QMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTH
HHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
YPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALFKAQARHSELAQNCLIQIADF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
HHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
ILDVDPQDLS
EEECCCCCCC
>Mature Secondary Structure
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLK
CHHHHHHHHCCHHHHHHHHCCCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
AILQPLDLAPLFTPIYWDWLHWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRIT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCHHHCCCCEEEEEE
DAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSAIWSALKAKGFIEEITIQTKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHEEEECCC
LSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
CCHHHCCCCCCEECCCCCEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAI
HHHHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCCCCEE
VIGTRSALFTQFSNLGAIILDEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATP
EEECHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCC
SLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHIQNGLSKPLLERMKAHLEKGN
CHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
QVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
EEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHHCCC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGT
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEHH
QMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTH
HHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
YPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALFKAQARHSELAQNCLIQIADF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
HHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
ILDVDPQDLS
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]