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The map label for this gene is pth [H]

Identifier: 148825325

GI number: 148825325

Start: 196483

End: 196974

Strand: Direct

Name: pth [H]

Synonym: CGSHiEE_01025

Alternate gene names: 148825325

Gene position: 196483-196974 (Clockwise)

Preceding gene: 148825319

Following gene: 148825326

Centisome position: 10.84

GC content: 38.62

Gene sequence:

>492_bases
TTGGCTCGTCGTTTTAACGTTTCACTAAATCCAGAAAGTAAATTCTTCGGAAAAACTGCCCGCACTTTAGTGAATGGAAA
AGAAGTACGCCTTTTAGTGCCAACAACTTTTATGAATTTAAGCGGTAAAGCAGTTGGCGCGTTGGCAAGTTTTTACCGTA
TTAAACCTGAAGAAATTTTGGTTATTCACGATGAACTAGATTTGCCTGCTGGCACAGCGAAACTCAAACAAGGCGGTGGA
CATGGTGGGCATAACGGCTTAAAAGATATTGTGGCTCAATTAGGCAATAACAATAATTTCTACCGTTTACGTATTGGCAT
TGGGCATCCTGGTCATCGTGATTTAGTGGCAGGTTATGTTTTAAATAAACCATCTCCAGCAGATCGCGATGCACTTGAAA
AAGTGTTAGATGAGGCAACTGATTGTGTAGAAATAATTTTCAAAGATGGAATGATCAAAGCGACCAATCGTTTAAACAGT
TTTAAAATTTAA

Upstream 100 bases:

>100_bases
ATAAAAATGTCAGAAATAAAACTTAATTGTTGGTTTAGGCAACCCAGGCGAAAAATATGCCGATACACGCCATAATGCAG
GCGAATGGCTTATTGAACGC

Downstream 100 bases:

>100_bases
AAGTGCGGTTAGTTTTACAACCGTTTTACTCAAAATAAGGAAAAATTATGGGATTCAAATGTGGTATTGTGGGATTGCCA
AATGTGGGCAAATCTACTCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MARRFNVSLNPESKFFGKTARTLVNGKEVRLLVPTTFMNLSGKAVGALASFYRIKPEEILVIHDELDLPAGTAKLKQGGG
HGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRDALEKVLDEATDCVEIIFKDGMIKATNRLNS
FKI

Sequences:

>Translated_163_residues
MARRFNVSLNPESKFFGKTARTLVNGKEVRLLVPTTFMNLSGKAVGALASFYRIKPEEILVIHDELDLPAGTAKLKQGGG
HGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRDALEKVLDEATDCVEIIFKDGMIKATNRLNS
FKI
>Mature_162_residues
ARRFNVSLNPESKFFGKTARTLVNGKEVRLLVPTTFMNLSGKAVGALASFYRIKPEEILVIHDELDLPAGTAKLKQGGGH
GGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYVLNKPSPADRDALEKVLDEATDCVEIIFKDGMIKATNRLNSF
KI

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=145, Percent_Identity=37.2413793103448, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1787455, Length=162, Percent_Identity=62.3456790123457, Blast_Score=193, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 17853; Mature: 17722

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRFNVSLNPESKFFGKTARTLVNGKEVRLLVPTTFMNLSGKAVGALASFYRIKPEEIL
CCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECEEEECCCCHHHHHHHHHHCCCCCEEE
VIHDELDLPAGTAKLKQGGGHGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYV
EEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHEE
LNKPSPADRDALEKVLDEATDCVEIIFKDGMIKATNRLNSFKI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure 
ARRFNVSLNPESKFFGKTARTLVNGKEVRLLVPTTFMNLSGKAVGALASFYRIKPEEIL
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECEEEECCCCHHHHHHHHHHCCCCCEEE
VIHDELDLPAGTAKLKQGGGHGGHNGLKDIVAQLGNNNNFYRLRIGIGHPGHRDLVAGYV
EEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHEE
LNKPSPADRDALEKVLDEATDCVEIIFKDGMIKATNRLNSFKI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA