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The map label for this gene is tilS [H]

Identifier: 148825315

GI number: 148825315

Start: 187151

End: 188443

Strand: Direct

Name: tilS [H]

Synonym: CGSHiEE_00975

Alternate gene names: 148825315

Gene position: 187151-188443 (Clockwise)

Preceding gene: 148825314

Following gene: 148825319

Centisome position: 10.32

GC content: 38.59

Gene sequence:

>1293_bases
ATGGATTTACTTTCAGACATTGAAAAACAACTCCAAAAAGCCACCGCTCAAGGGTTTCTTATCGCCTTGAGTGGTGGTTT
AGATTCCACTGTACTTCTTTCTTTATTTGCAAAACTTCGTCAAAAACAACCGCACTTACCGCCTTTATCAGTACGAGCAA
TCCACATTCATCACGGCTTAAGCCCCAATGCAGACAGTTGGGCTAAACATTGCCAAGATTTATGCGATCAATTTCAAATT
CCGTTGATTATCGAGCGAGTACAAGTAGATAAAACTAATGGCATTGAGGCTGGCGCACGTGAAGCACGCTATCAAGCCAT
CAAAAAACATCTTCAAACACAAGAAATACTCGTCACGGCACATCATCTAAATGATCAAACAGAAACCTTTTTCTTAGCCC
TAAAACGTGGCAGTGGGCTACAAGGCTTAGGCGCAATGCAACAACAAAGTGTATTGTTCGGCATGCCAATTTTACGCCCT
TTGTTAGGCTTTACTCGCACCCAGTTAGAAAATTACGCACAAAAAGAAAAACTCAACTGGATTACAGATGAAAGTAATGG
GGATAATCGATTTGATCGTAATTTCTTACGCAATGAAATTTTGCCAAAATTACGTGAACGCTGGGCTTATTTTGATTTAG
CCGTGCAACGTTCCGCACAACATTGTTTTGAGCAACAACAGCTAATTAATGATTTATTGAGTGAAGCCTTTGCAGAACAT
TGTCAAATTAAAAACCAATTTAAACTGTGCCAGTTCCGAAAATATTCTCTCGCAAAACAAACCGCACTTTTGCGTATGTG
GCTTGCGGAAAACCAACTTGAAATGCCAAGTAAACGCCAGCTTACACAGCTTATTAATGATGTTGTTTTTGCAAAAGAAG
AGGCAAATCCTCAATTTCAGCTTGTAAATAAAGTCATTCGACGTTATCAAGATCGTTTATATTTAACTAAGCCGTTTTCT
GATCTAACCAAATACTGCCTAAAATTAGAACAAAACACGTTAAGTTTACCCGATGATTTAGGCAATCTCAGTGTTCAAGA
AAATGAACATAATCTGATTTTCCACTGGCAGGATTTTTCTGTGACGCTGGAAAAAACGAACTTGCCTATTTCTATTCGTT
TTGGCTATTCGGGTAAAGTGAAACATCATCTTAAACGCCCAAGAGAAGATATAAAAAAAATCTGGCAAGAATTAAGCGTG
CCACCTTGGGAACGAAATCGGATCCCTTTAATTTTTTATGGCAACAAGCTAAAAAGTGCGGTCGGTTTTTTCCGTGTTTT
TGACGAGTATTAA

Upstream 100 bases:

>100_bases
AAACCACCTTTGAACTGGGTTCTTACGAGTTACAAACCATTGCTGCACGTTTTGAAATTCTTAATCCAAGCAGTAACTAT
AAAGCTGAAAAAGTTGCCTA

Downstream 100 bases:

>100_bases
AATCAAGTTAAAGCGGCTTCGTTTCTAAAAATGCGTTCAAAATTTGAGCAGTAAATTCTTTTCTTAGATAAAAACCTAAA
GGCAAGGTATCTATCACTTC

Product: pyridoxine kinase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDLLSDIEKQLQKATAQGFLIALSGGLDSTVLLSLFAKLRQKQPHLPPLSVRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKHLQTQEILVTAHHLNDQTETFFLALKRGSGLQGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNGDNRFDRNFLRNEILPKLRERWAYFDLAVQRSAQHCFEQQQLINDLLSEAFAEH
CQIKNQFKLCQFRKYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVVFAKEEANPQFQLVNKVIRRYQDRLYLTKPFS
DLTKYCLKLEQNTLSLPDDLGNLSVQENEHNLIFHWQDFSVTLEKTNLPISIRFGYSGKVKHHLKRPREDIKKIWQELSV
PPWERNRIPLIFYGNKLKSAVGFFRVFDEY

Sequences:

>Translated_430_residues
MDLLSDIEKQLQKATAQGFLIALSGGLDSTVLLSLFAKLRQKQPHLPPLSVRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKHLQTQEILVTAHHLNDQTETFFLALKRGSGLQGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNGDNRFDRNFLRNEILPKLRERWAYFDLAVQRSAQHCFEQQQLINDLLSEAFAEH
CQIKNQFKLCQFRKYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVVFAKEEANPQFQLVNKVIRRYQDRLYLTKPFS
DLTKYCLKLEQNTLSLPDDLGNLSVQENEHNLIFHWQDFSVTLEKTNLPISIRFGYSGKVKHHLKRPREDIKKIWQELSV
PPWERNRIPLIFYGNKLKSAVGFFRVFDEY
>Mature_430_residues
MDLLSDIEKQLQKATAQGFLIALSGGLDSTVLLSLFAKLRQKQPHLPPLSVRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKHLQTQEILVTAHHLNDQTETFFLALKRGSGLQGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNGDNRFDRNFLRNEILPKLRERWAYFDLAVQRSAQHCFEQQQLINDLLSEAFAEH
CQIKNQFKLCQFRKYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVVFAKEEANPQFQLVNKVIRRYQDRLYLTKPFS
DLTKYCLKLEQNTLSLPDDLGNLSVQENEHNLIFHWQDFSVTLEKTNLPISIRFGYSGKVKHHLKRPREDIKKIWQELSV
PPWERNRIPLIFYGNKLKSAVGFFRVFDEY

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=414, Percent_Identity=42.0289855072464, Blast_Score=312, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50091; Mature: 50091

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLSDIEKQLQKATAQGFLIALSGGLDSTVLLSLFAKLRQKQPHLPPLSVRAIHIHHGL
CCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
SPNADSWAKHCQDLCDQFQIPLIIERVQVDKTNGIEAGAREARYQAIKKHLQTQEILVTA
CCCHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
HHLNDQTETFFLALKRGSGLQGLGAMQQQSVLFGMPILRPLLGFTRTQLENYAQKEKLNW
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITDESNGDNRFDRNFLRNEILPKLRERWAYFDLAVQRSAQHCFEQQQLINDLLSEAFAEH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQIKNQFKLCQFRKYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVVFAKEEANPQFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LVNKVIRRYQDRLYLTKPFSDLTKYCLKLEQNTLSLPDDLGNLSVQENEHNLIFHWQDFS
HHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCE
VTLEKTNLPISIRFGYSGKVKHHLKRPREDIKKIWQELSVPPWERNRIPLIFYGNKLKSA
EEEEECCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
VGFFRVFDEY
HHHHHHHHCC
>Mature Secondary Structure
MDLLSDIEKQLQKATAQGFLIALSGGLDSTVLLSLFAKLRQKQPHLPPLSVRAIHIHHGL
CCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
SPNADSWAKHCQDLCDQFQIPLIIERVQVDKTNGIEAGAREARYQAIKKHLQTQEILVTA
CCCHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
HHLNDQTETFFLALKRGSGLQGLGAMQQQSVLFGMPILRPLLGFTRTQLENYAQKEKLNW
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITDESNGDNRFDRNFLRNEILPKLRERWAYFDLAVQRSAQHCFEQQQLINDLLSEAFAEH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQIKNQFKLCQFRKYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVVFAKEEANPQFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LVNKVIRRYQDRLYLTKPFSDLTKYCLKLEQNTLSLPDDLGNLSVQENEHNLIFHWQDFS
HHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCE
VTLEKTNLPISIRFGYSGKVKHHLKRPREDIKKIWQELSVPPWERNRIPLIFYGNKLKSA
EEEEECCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
VGFFRVFDEY
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA