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The map label for this gene is gidB [H]

Identifier: 148825234

GI number: 148825234

Start: 107141

End: 107752

Strand: Direct

Name: gidB [H]

Synonym: CGSHiEE_00550

Alternate gene names: 148825234

Gene position: 107141-107752 (Clockwise)

Preceding gene: 148825233

Following gene: 148825235

Centisome position: 5.91

GC content: 31.86

Gene sequence:

>612_bases
ATGAAAGCCAAATTAGTCAGTTTACTTGCACAAGCAAATATAAAAATAAGTGATCAACAAATTCAGCAGTTGATTGATCT
GGTCAATTTACTCAATAAATGGAATAAAGCTTATAATTTGACGTCAGTTCGTGATCCACAAGAAATGTTAGTCAAACATA
TTTTAGATAGTCTTGTAGTAAGTCCTTATTTACAGGGTGATCGTTTTATTGATGTCGGTACTGGTCCTGGCTTGCCTGGT
TTACCTTTAGCAATTATTAATCCTTCCAAACAATTTGTTCTTCTTGATAGTTTAGGTAAGCGTATCAGCTTTATTAGAAA
TGCTATACGTGAGCTTAGACTTACTAATGCAACCCCTGTTTTAAGTAGAGTAGAAGAATATCAACCTGAAGATAAATTTG
ATGGTGTACTCAGTCGTGCTTTTGCTTCACTAAAAGATATGACAGATTGGTGTTATCATTTACCAAAAGAAAATGGATAT
TTTTATGCATTAAAAGGGATTTATCAGGAAGATGAAATTAATGAATTGAATAAAAAATATACTATTCAAAAAGTGATTGA
ATTATCTGTTCCAGAGCTTATAGGCGAACGACATTTAATCGTTTTACGTTAA

Upstream 100 bases:

>100_bases
ACATTGATTCGGTAAATAATTCATCAAATTACTAAAAACATTAAGGAAATTTACCGCACTTTTAAGTAAAATAGCGTAAT
TTTTCAAAGAGATAATAAAT

Downstream 100 bases:

>100_bases
ATAGTTACAATTTAGTAAAAAATTTCTAAAAGTGTGATTTAGTTAACGTTTTTCAGTGCTTTTTAAGTTGAATCTAATTT
GATCAAATGTATAATTAGAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKAKLVSLLAQANIKISDQQIQQLIDLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNATPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCYHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR

Sequences:

>Translated_203_residues
MKAKLVSLLAQANIKISDQQIQQLIDLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNATPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCYHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR
>Mature_203_residues
MKAKLVSLLAQANIKISDQQIQQLIDLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNATPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCYHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=203, Percent_Identity=63.0541871921182, Blast_Score=268, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23177; Mature: 23177

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKLVSLLAQANIKISDQQIQQLIDLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGDRFIDVGTGPGLPGLPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNATPV
HHCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHH
LSRVEEYQPEDKFDGVLSRAFASLKDMTDWCYHLPKENGYFYALKGIYQEDEINELNKKY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
TIQKVIELSVPELIGERHLIVLR
HHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKAKLVSLLAQANIKISDQQIQQLIDLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGDRFIDVGTGPGLPGLPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNATPV
HHCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHH
LSRVEEYQPEDKFDGVLSRAFASLKDMTDWCYHLPKENGYFYALKGIYQEDEINELNKKY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
TIQKVIELSVPELIGERHLIVLR
HHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA