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The map label for this gene is aspA [H]

Identifier: 148825192

GI number: 148825192

Start: 57522

End: 58940

Strand: Direct

Name: aspA [H]

Synonym: CGSHiEE_00320

Alternate gene names: 148825192

Gene position: 57522-58940 (Clockwise)

Preceding gene: 148825191

Following gene: 148825196

Centisome position: 3.17

GC content: 37.63

Gene sequence:

>1419_bases
ATGACTCAATTTAGAAAAGAAGTAGATTTACTCGGCGAACGTGATGTGCCTGCAGAAGAATATTGGGGGATTCATACATT
AAGAGCGGTAGAAAATTTCAATATTTCTAACGTAACCATTTCTGACGTACCAGAGTTTGTACGTGGTATGGTAATGGTAA
AAAAAGCAACAGCTTTAGCCAATGGCGAATTAGGTGCAATTCCAAGTGATATTGCAAAAGCGATTGTAGCAGCTTGTGAT
GAAATCCTTACTACTGGAAAATGCTTGGATCAATTCCCATCAGATGTATATCAAGGTGGTGCAGGCACTTCTGTCAATAT
GAATACTAATGAAGTGGTTGCTAATCTTGCCCTTGAAAAAATTGGTCATAAAAAAGGCGAATATAACGTGATTAATCCGA
TGGATCACGTTAATGCAAGCCAATCAACCAACGATGCGTATCCTACTGGTTTCCGTATTGCGGTGTATAACAGCATCTTA
AAATTGATCGATAAAATTCAATATTTACACGACGGTTTTGACAATAAAGCAAAAGAGTTTGCAAATATCTTAAAAATGGG
TCGTACCCAATTGCAAGATGCGGTGCCAATGACTGTTGGTCAAGAATTCAAAGCTTTCGCCGTATTACTTGAAGAAGAAG
TGCGTAACTTAAAACGTACAGCAGGTTTATTACTTGAAATAAACCTTGGTGCGACTGCAATCGGTACTGGTTTAAATACG
CCACAAGGCTATACAGAATTAGTTGTAAAACATCTTGCTGAAGTGACTGGATTAGCTTGTGTACCAGCAGAAAACTTAAT
TGAAGCAACATCTGACTGTGGTGCTTATGTCATGGTTCACGGTGCATTAAAACGTACTGCAGTGAAACTTTCTAAAGTAT
GTAATGACTTACGTTTACTTTCTTCTGGCCCTCGTGCAGGTTTAAAAGAAATTAATCTTCCTGAATTACAAGCAGGTTCT
TCTATTATGCCAGCAAAAGTAAACCCAGTTGTTCCTGAAGTGGTGAACCAAGTATGCTTTAAAGTAATTGGTAACGATAC
CACTGTGACTTTCGCATCTGAAGCTGGTCAATTACAATTAAACGTAATGGAACCCGTGATTGGTCAAGCAATGTTTGAAT
CTATCGACATCTTAACCAATGCTTGTGTGAACTTACGCGATAAATGCGTGGATGGCATCACGGTAAACAAAGAAATTTGT
GAAAACTACGTGTTTAATTCAATCGGTATCGTGACTTACTTAAATCCATTTATTGGTCACCACAACGGCGACTTAGTGGG
TAAAATCTGTGCACAAACAGGCAAAGGCGTACGTGAAGTTGTGTTAGAAAAAGGTTTATTAACAGAAGAACAATTAGATG
ACATTCTTTCTGTAGAAAACTTAATGAATCCAACTTACAAAGCGAAATTAAATAAATAA

Upstream 100 bases:

>100_bases
GTCTTGAGAGCATTTTTTGAAAATGTGATCTTCATCAAGTTTTTTCTTCTTGAAATTTCTATAATGACGATAAATTAACT
AACCCAAAAAGGTGATTCAA

Downstream 100 bases:

>100_bases
TTTACGCCAAATATCAAAAAAATTTACCGCACTTTAATCTGTGCCTTAAAGCTGAATAAATAAACCAGCTAATTGGGGCG
CAGATTTTTTTATGATTAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTQFRKEVDLLGERDVPAEEYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACD
EILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSIL
KLIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEINLGATAIGTGLNT
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEIC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Sequences:

>Translated_472_residues
MTQFRKEVDLLGERDVPAEEYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACD
EILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSIL
KLIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEINLGATAIGTGLNT
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEIC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
>Mature_471_residues
TQFRKEVDLLGERDVPAEEYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACDE
ILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSILK
LIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEINLGATAIGTGLNTP
QGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGSS
IMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICE
NYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=40.4761904761905, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=78.3261802575107, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=37.3650107991361, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1790398, Length=282, Percent_Identity=23.0496453900709, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=40.6451612903226, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.1042944785276, Blast_Score=257, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=40.0862068965517, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=40, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=40, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=38.7931034482759, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.0086206896552, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24583245, Length=449, Percent_Identity=32.739420935412, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51181; Mature: 51049

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFRKEVDLLGERDVPAEEYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALA
CCHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
NGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
IGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDGFDNKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEINLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
CCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure 
TQFRKEVDLLGERDVPAEEYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALA
CHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
NGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
IGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDGFDNKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEINLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
CCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]