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The map label for this gene is gidB [H]

Identifier: 148825127

GI number: 148825127

Start: 4420532

End: 4421206

Strand: Reverse

Name: gidB [H]

Synonym: TBFG_13954

Alternate gene names: 148825127

Gene position: 4421206-4420532 (Counterclockwise)

Preceding gene: 148825128

Following gene: 148825126

Centisome position: 99.93

GC content: 66.07

Gene sequence:

>675_bases
ATGTCTCCGATCGAGCCCGCGGCGTCTGCGATCTTCGGACCGCGGCGTGGCCTTGCTCGGCGGTACGCCGAAGCGTTGGC
GGGACCCGGTGTGGAGCGGGGGCTGGTGGGACCCCGCGAAGTCGGTAGGCTATGGGACCGGCATCTACTGAACTGCGCCG
TGATCGGTGAGCTCCTCGAACGCGGTGACCGGGTCGTGGATATCGGTAGCGGAGCCGGGTTGCCGGGCGTGCCATTGGCG
ATAGCGCGGCCGGACCTCCAGGTAGTTCTCCTAGAACCGCTACTGCACCGCACCGAGTTTCTTCGAGAGATGGTGACAGA
TCTGGGCGTGGCCGTTGAGATCGTGCGGGGGCGCGCCGAGGAGTCCTGGGTGCAGGACCAATTGGGCGGCAGCGACGCTG
CGGTGTCACGGGCGGTGGCCGCGTTGGACAAGTTGACGAAATGGAGCATGCCGTTGATACGGCCGAACGGGCGAATGCTC
GCCATCAAAGGCGAGCGGGCTCACGACGAAGTACGGGAGCACCGGCGTGTGATGATCGCATCGGGCGCGGTTGATGTCAG
GGTGGTGACATGTGGCGCGAACTATTTGCGTCCGCCCGCGACCGTGGTGTTCGCACGACGTGGAAAGCAGATCGCCCGAG
GGTCGGCACGGATGGCGAGTGGAGGGACGGCGTGA

Upstream 100 bases:

>100_bases
AGTAAGCGATGCGTGGCCGAGCGGCTGGGCCAGCGTCTCGAGAGCGGAGAATGTTTCACGTGAAACATGACACAGACCTC
ACGAGCCGGCGGAGTGCGTA

Downstream 100 bases:

>100_bases
GTGCTCCGTGGGGCCCGGTGGCCGCTGGACCGTCCGCGCTCGTAAGGTCGGGCCAGGCTTCAACTATCGAACCATTCCAG
CGGGAAATGACACCACCGAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSPIEPAASAIFGPRRGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLA
IARPDLQVVLLEPLLHRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRML
AIKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA

Sequences:

>Translated_224_residues
MSPIEPAASAIFGPRRGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLA
IARPDLQVVLLEPLLHRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRML
AIKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
>Mature_223_residues
SPIEPAASAIFGPRRGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLAI
ARPDLQVVLLEPLLHRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLA
IKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA

Specific function: Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=151, Percent_Identity=33.7748344370861, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 24056; Mature: 23925

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIEPAASAIFGPRRGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLE
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGDRVVDIGSGAGLPGVPLAIARPDLQVVLLEPLLHRTEFLREMVTDLGVAVEIVRGRAE
CCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLAIKGERAHDEVREHRRVMIA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHEEEEE
SGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
CCCEEEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SPIEPAASAIFGPRRGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLE
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGDRVVDIGSGAGLPGVPLAIARPDLQVVLLEPLLHRTEFLREMVTDLGVAVEIVRGRAE
CCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLAIKGERAHDEVREHRRVMIA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHEEEEE
SGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
CCCEEEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]