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The map label for this gene is dfrA [H]

Identifier: 148824930

GI number: 148824930

Start: 4183215

End: 4184144

Strand: Direct

Name: dfrA [H]

Synonym: TBFG_13757

Alternate gene names: 148824930

Gene position: 4183215-4184144 (Clockwise)

Preceding gene: 148824929

Following gene: 148824931

Centisome position: 94.55

GC content: 63.66

Gene sequence:

>930_bases
ATGCAGAATGCCACCATGCGCGTTCTGGTCACCGGCGGTACGGGATTTGTGGGCGGGTGGACTGCCAAAGCCATCGCTGA
CGCGGGCCACTCCGTCCGGTTCCTGGTGCGAAATCCCGCACGGCTGAAGACGTCTGTCGCGAAACTGGGCGTCGACGTGT
CGGACTTTGCGGTTGCAGACATATCCGACCGCGATTCGGTACGGGAGGCGTTGAACGGATGCGACGCCGTCGTGCACAGC
GCCGCGCTGGTGGCAACCGACCCGCGTGAGACTTCGCGGATGCTGAGTACGAACATGGCGGGCGCCCAAAATGTTCTCGG
TCAAGCCGTCGAGCTCGGAATGGATCCGATCGTGCATGTGTCGAGCTTCACGGCGCTGTTTCGTCCCAACTTGGCGACGC
TGAGCGCTGATCTGCCGGTTGCCGGTGGGACGGATGGATACGGACAATCCAAAGCGCAGATCGAAATCTATGCGCGCGGT
CTTCAGGACGCCGGCGCACCGGTGAACATCACTTATCCTGGCATGGTCCTCGGCCCGCCGGTGGGCGATCAATTCGGTGA
AGCCGGGGAGGGTGTCCGGTCCGCATTGTGGATGCATGTCATTCCCGGGCGCGGCGCGGCGTGGTTGATCGTCGACGTCC
GAGATGTGGCGGCACTGCACGCGGCGTTGTTGGAATCCGGGCGTGGGCCGCGCCGCTACACTGCGGGAGGTCATCGGATT
CCGGTGCCCGAGCTCGCGAAAATTCTGGGCGGGTCGCCGGCACCACGATGCTGGCCGTCCCGGTGCCCGATTCCGCGCTG
CGTGTCGCGGGATCGGTGCTGGATCAAGCCGGGCCCTATCTGCCTTTCAATACTCCGTTCACCGCGGCAGGTATGCAGTA
CTACACACAGATGCCGGAGTCCGACGATTCGCCGAGCGAAAAAGAACTAG

Upstream 100 bases:

>100_bases
ACGCGGTGAACGGCATGGTAGGCCAGGGCGCGAATTTCGCCGCCAGCCGCCTGTAGCCAGAACTGCGCTGCCACCCCAGC
GAGAGCTGGGCGGTGATCCA

Downstream 100 bases:

>100_bases
GCATCACCTACCGCGATCCGCGCGACACCGTGGCCGACACCGTCACGGCCCTGCGCGGCCTGGGCAGCTAACTGCCGTCG
GGAGGTTCCGCCGGTTCCGC

Product: oxidoreductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVADISDRDSVREALNGCDAVVHS
AALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHVSSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARG
LQDAGAPVNITYPGMVLGPPVGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRI
PVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRSPTIRRAKKN

Sequences:

>Translated_309_residues
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVADISDRDSVREALNGCDAVVHS
AALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHVSSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARG
LQDAGAPVNITYPGMVLGPPVGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRI
PVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRSPTIRRAKKN
>Mature_309_residues
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVADISDRDSVREALNGCDAVVHS
AALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHVSSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARG
LQDAGAPVNITYPGMVLGPPVGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRI
PVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRSPTIRRAKKN

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 32726; Mature: 32726

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVAD
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCHHHHEEEC
ISDRDSVREALNGCDAVVHSAALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHV
CCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHH
SSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARGLQDAGAPVNITYPGMVLGPP
HHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEECHHCCCCCEEEECCCEEECCC
VGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRI
CCCHHCHHHHHHHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRS
CHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCC
PTIRRAKKN
CHHHHCCCC
>Mature Secondary Structure
MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPARLKTSVAKLGVDVSDFAVAD
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCHHHHEEEC
ISDRDSVREALNGCDAVVHSAALVATDPRETSRMLSTNMAGAQNVLGQAVELGMDPIVHV
CCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHH
SSFTALFRPNLATLSADLPVAGGTDGYGQSKAQIEIYARGLQDAGAPVNITYPGMVLGPP
HHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEECHHCCCCCEEEECCCEEECCC
VGDQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAALLESGRGPRRYTAGGHRI
CCCHHCHHHHHHHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVPELAKILGGSPAPRCWPSRCPIPRCVSRDRCWIKPGPICLSILRSPRQVCSTTHRCRS
CHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCC
PTIRRAKKN
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]