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The map label for this gene is arsB [H]

Identifier: 148824784

GI number: 148824784

Start: 4032923

End: 4034164

Strand: Direct

Name: arsB [H]

Synonym: TBFG_13611

Alternate gene names: 148824784

Gene position: 4032923-4034164 (Clockwise)

Preceding gene: 148824783

Following gene: 148824790

Centisome position: 91.15

GC content: 69.73

Gene sequence:

>1242_bases
TTGACACTTGCTGTTGCGCTGATACTGCTTGCCGTGGTCCTGGGGTTCGCGGTTGCCCGCCCACGCGGCTGGCCGGAGGC
AGCGGCGGCGGTTCCGGCAGCGGTCATCCTGTTAGCGATCGGGGCGATCTCGCCCCAGCAGGCGATGGCGCAGGTGTCCG
GGCTGGCGCGCGTGGTCGCGTTTCTGGGTGCGGTTCTGGTGCTGGCTAAGCTGTGCGACGACGAAGGCCTGTTCGAGGCA
GCCGGCGCGGCCATGGCTCGAGCGAGCGCGGAGTCGCACCGACTGCTACGGCAGGTGTTCGCCGTCTCGGCCGCCATCAC
CGCGGCGCTCTGCCTGGACGCCACCGTGGTGCTGTTGACCCCGGTGGTGCTGGCGACGGTCCGCCGGCTGCGGACCCCGG
TGCGCCCCTATGCCTACGCCACCGCCCACCTAGCCAACGCCGCTTCGCTGCTGCTTCCGGTGTCGAATCTGACCAACCTG
CTCGCCTACCACGGTGCCGGCATCTCGTTCACCAAGTTCACGCTGCTGATGGCATTGCCTTGGCTGTCCGCCGTGGCCGC
GGTCTATGTGGTCTTCCGCTGGTTTTTCGCCCGGGATCTACGCGTGGTGCCGGACCGGCAGCAACTCAAGCCGGCGCCGC
GCCTGCCAATGTTCGTGCTGGTGGTGGTGGCGCTGACACTCGGGGGCTTCGCCGTCGCCGAGTCGGTGGGACTGGCCCCA
ACGTGGGCGGCGCTGGCTGGCGCCGCAGTGTTGGCGCTGCGAAGTCTGCGGCGTGGACACACTTCGGTGCTGCGGATCGC
GCGCGCCGTCAACGTGTCGTTCCTGGTCTTTGTGTTGGCCCTGGGTGTCGTGGTGCACGCGGTCATGCTCAACGGCATGG
CCGCCAGGATGTCCGCCGTGCTGCCGACCGGGTCCGGGTTGCCCGCGCTGCTCGGCATCGCCGCGCTGGCCGCCGTGCTG
GCCAACGTGGTCAACAACCTGCCCGCGACTCTGGTGTTAGTGCCGCTGGTGGCGGCCGGCGGGCCGGCGGCCGTGCTGGC
CGTGCTACTCGGGGTCAACATCGGACCCAACCTGACCTATGCCGGTTCGCTGTCTAACCTGCTGTGGCGGGGCGTGCTGC
GCCGGCACAACGTCGACGCCAGCGTCGGCGAGTACACCCGACTGGGACTGTGCACCGTGCCTGCGGCCCTGGCGATGGCG
GTGCTCGCGCTGTGGGCCAGCGCCCAGGTTCTGGGGATCTAG

Upstream 100 bases:

>100_bases
ATCCGCATCCTCGACGAGCTGGCCGCCCAGGACGGCGTCGCGCTGCAGATGCCGACGGTGTGGCGCCGCGAGGATCCCTG
GATGTGAAGCGCTCTAGCCC

Downstream 100 bases:

>100_bases
CCGCAAGGGCGCGAGCAGACGCAGAATCGCATGATTTGAGCTCAAATCATGCGATTCTGCGTCTGCTCGCGAGGCTCGCG
TGGCCGCCGGCGCTGGCGGG

Product: arsenical pump integral membrane protein arsB2

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEA
AGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNL
LAYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALAAVL
ANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMA
VLALWASAQVLGI

Sequences:

>Translated_413_residues
MTLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEA
AGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNL
LAYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALAAVL
ANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMA
VLALWASAQVLGI
>Mature_412_residues
TLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEAA
GAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNLL
AYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAPT
WAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALAAVLA
NVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMAV
LALWASAQVLGI

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=415, Percent_Identity=30.3614457831325, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 42544; Mature: 42412

Theoretical pI: Translated: 11.71; Mature: 11.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FLGAVLVLAKLCDDEGLFEAAGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNLLAYHGAGISFTKFTLLMALP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTGSGLPALLGIAALAAVLANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
AGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMAVLALWASAQVLGI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]