Click here to switch to the map view.

The map label for this gene is nat [H]

Identifier: 148824772

GI number: 148824772

Start: 4020112

End: 4021500

Strand: Reverse

Name: nat [H]

Synonym: TBFG_13599

Alternate gene names: 148824772

Gene position: 4021500-4020112 (Counterclockwise)

Preceding gene: 148824773

Following gene: 148824766

Centisome position: 90.89

GC content: 64.15

Gene sequence:

>1389_bases
GTGGCGCGAGCAGACGCAAAATCGCCCATTTTGCGGGCGTGTTGGGCGATTTTGCGTCTGCTCGCCGTCGCCGGTAAGCA
GTCTGGTGAGCCACGTCCGGTATGTCGCGTGGTTGGGGCAGCTGTCCTCGCGGATGGCGAATATGCAGACGCTTACGTCA
AGGAGCAGCATTGTCGCCGATCAGTTCGGCGAGGGCTTCCTTGTCGTCCAAGTCGACACCGGCCTGCAAGCCTCCGGTGC
CGTGGGTCGGCAATAGCATCAGTAACAGGCATCATGATGCGTTGTTGTGTCCGGCGCCGATCCTCCGACCCGCCGGGCAT
TCGGCCAGATGGCGCGAGCAGCCACCGGATGGGTGAGTGTCAGCGGGCAGTTTGCCGTAGCCGCCGACACGTGTCGCTGC
GAGGGCACTTTGTTTGCCGTCGACCCAGAAACTCATGTGGCAAACCACAATCGGTGCGACATAGTTGGCCGGCTGCGCGA
CGAACGCCCGAATACCCTGCGGTCGGTCCGACGCGGCGACGAGGTCAGAATGGCAACATGGCACTGGATCTGACCGCGTA
CTTCGATCGCATCAACTATCGCGGCGCTACCGATCCAACCCTGGATGTTCTGCAGGATCTGGTGACCGTGCACAGTCGAA
CGATTCCGTTCGAGAACCTCGACCCGCTGCTGGGGGTGCCGGTCGACGACCTCAGTCCACAGGCGCTGGCCGACAAGCTG
GTACTTCGGCGCCGAGGCGGGTACTGCTTTGAGCACAACGGGCTGATGGGTTATGTGCTGGCCGAACTCGGCTATCGGGT
GCGCCGATTCGCCGCCCGCGTCGTCTGGAAGCTCGCGCCGGACGCGCCCCTGCCGCCGCAGACGCACACCCTGCTGGGGG
TCACGTTCCCCGGCTCGGGCGGATGCTATCTCGTCGACGTCGGATTCGGCGGCCAAACACCGACCTCACCGCTTCGCCTC
GAAACCGGCGCCGTCCAGCCGACAACGCACGAACCTTATCGGCTCGAGGACCGCGTCGACGGCTTTGTCTTGCAGGCGAT
GGTCCGGGACACATGGCAGACACTGTACGAATTCACCACCCAGACCCGCCCGCAGATCGATCTGAAAGTGGCCAGCTGGT
ACGCCTCAACACACCCGGCATCGAAGTTCGTCACGGGACTGACCGCCGCGGTGATCACCGACGACGCCCGGTGGAACCTA
TCTGGCCGCGACCTTGCCGTTCACCGTGCCGGTGGTACCGAGAAGATCCGCCTTGCCGATGCGGCAGCGGTTGTCGACAC
CCTGAGCGAACGGTTCGGGATCAACGTGGCAGATATCGGCGAGCGCGGCGCGCTCGAGACGCGCATCGACGAGCTATTGG
CTCGGCAGCCAGGAGCCGATGCGCCGTAA

Upstream 100 bases:

>100_bases
GCGACTACACCGGCATCGAGCCGGAAAAGACCACGCCGGCGCACTGGCGCGACGACCTGGAGGCTTTCCTCATCACCACC
ACCCAGGACACCTGGCTCTA

Downstream 100 bases:

>100_bases
GGCTTCTTCGATGTCGCCGCTTGGCCCGGCAAACGATATCCGAACAAACGAACCCCCCCGTGCGGTGTCGAAATCGATTC
CGGGTGCGATCGCAACACCG

Product: arylamine n-acetyltransferase nat (arylamine acetylase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MARADAKSPILRACWAILRLLAVAGKQSGEPRPVCRVVGAAVLADGEYADAYVKEQHCRRSVRRGLPCRPSRHRPASLRC
RGSAIASVTGIMMRCCVRRRSSDPPGIRPDGASSHRMGECQRAVCRSRRHVSLRGHFVCRRPRNSCGKPQSVRHSWPAAR
RTPEYPAVGPTRRRGQNGNMALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKL
VLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRL
ETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNL
SGRDLAVHRAGGTEKIRLADAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP

Sequences:

>Translated_462_residues
MARADAKSPILRACWAILRLLAVAGKQSGEPRPVCRVVGAAVLADGEYADAYVKEQHCRRSVRRGLPCRPSRHRPASLRC
RGSAIASVTGIMMRCCVRRRSSDPPGIRPDGASSHRMGECQRAVCRSRRHVSLRGHFVCRRPRNSCGKPQSVRHSWPAAR
RTPEYPAVGPTRRRGQNGNMALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKL
VLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRL
ETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNL
SGRDLAVHRAGGTEKIRLADAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
>Mature_461_residues
ARADAKSPILRACWAILRLLAVAGKQSGEPRPVCRVVGAAVLADGEYADAYVKEQHCRRSVRRGLPCRPSRHRPASLRCR
GSAIASVTGIMMRCCVRRRSSDPPGIRPDGASSHRMGECQRAVCRSRRHVSLRGHFVCRRPRNSCGKPQSVRHSWPAARR
TPEYPAVGPTRRRGQNGNMALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKLV
LRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRLE
TGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLS
GRDLAVHRAGGTEKIRLADAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP

Specific function: Could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid [H]

COG id: COG2162

COG function: function code Q; Arylamine N-acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arylamine N-acetyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI237649132, Length=312, Percent_Identity=26.2820512820513, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI237649130, Length=312, Percent_Identity=26.2820512820513, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI237649128, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI237649126, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI237649124, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI237649122, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI237649119, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42741671, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI237649009, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI116295260, Length=265, Percent_Identity=26.0377358490566, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1787736, Length=259, Percent_Identity=39.3822393822394, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001447 [H]

Pfam domain/function: PF00797 Acetyltransf_2 [H]

EC number: =2.3.1.5 [H]

Molecular weight: Translated: 50838; Mature: 50707

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARADAKSPILRACWAILRLLAVAGKQSGEPRPVCRVVGAAVLADGEYADAYVKEQHCRR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SVRRGLPCRPSRHRPASLRCRGSAIASVTGIMMRCCVRRRSSDPPGIRPDGASSHRMGEC
HHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
QRAVCRSRRHVSLRGHFVCRRPRNSCGKPQSVRHSWPAARRTPEYPAVGPTRRRGQNGNM
HHHHHHHCCCEEEECEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCE
ALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKL
EEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSG
HHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
GCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFTT
CEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHH
QTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLAD
CCCCCEEEEEEEHECCCCCHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCCCEEEHHH
AAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARADAKSPILRACWAILRLLAVAGKQSGEPRPVCRVVGAAVLADGEYADAYVKEQHCRR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SVRRGLPCRPSRHRPASLRCRGSAIASVTGIMMRCCVRRRSSDPPGIRPDGASSHRMGEC
HHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
QRAVCRSRRHVSLRGHFVCRRPRNSCGKPQSVRHSWPAARRTPEYPAVGPTRRRGQNGNM
HHHHHHHCCCEEEECEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCE
ALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKL
EEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSG
HHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
GCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFTT
CEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHH
QTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLAD
CCCCCEEEEEEEHECCCCCHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCCCEEEHHH
AAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]