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The map label for this gene is otsA [H]

Identifier: 148824697

GI number: 148824697

Start: 3920589

End: 3922091

Strand: Direct

Name: otsA [H]

Synonym: TBFG_13524

Alternate gene names: 148824697

Gene position: 3920589-3922091 (Clockwise)

Preceding gene: 148824696

Following gene: 148824698

Centisome position: 88.61

GC content: 65.6

Gene sequence:

>1503_bases
ATGGCTCCCTCGGGAGGCCAGGAGGCGCAGATTTGCGATTCGGAGACCTTCGGGGACTCTGACTTCGTGGTGGTAGCCAA
TCGACTGCCCGTCGATCTGGAGCGTCTTCCCGACGGCAGCACAACCTGGAAACGCAGCCCCGGAGGCTTGGTCACCGCCT
TGGAGCCGGTGCTGCGGCGTCGGCGCGGGGCCTGGGTCGGCTGGCCCGGCGTTAACGACGACGGGGCCGAACCCGACCTC
CACGTGCTGGACGGCCCCATCATCCAAGACGAGCTGGAACTTCATCCGGTACGGCTGAGCACCACGGACATAGCTCAGTA
CTACGAGGGATTCTCCAACGCCACACTGTGGCCGCTGTACCACGACGTCATCGTCAAGCCGCTCTACCACCGCGAATGGT
GGGATCGCTACGTCGACGTCAACCAGCGCTTTGCCGAGGCCGCGTCGCGCGCCGCCGCCCACGGCGCAACCGTGTGGGTA
CAGGACTACCAGCTGCAGCTGGTACCGAAGATGCTGCGCATGCTGCGGCCCGATCTGACCATCGGTTTCTTTTTGCACAT
CCCGTTCCCGCCGGTAGAGCTGTTTATGCAGATGCCGTGGCGCACCGAGATCATCCAGGGCCTACTGGGCGCCGACCTGG
TGGGCTTCCATCTTCCGGGCGGTGCCCAGAATTTCCTGATCCTGTCCCGGCGTCTGGTCGGCACCGACACTTCCCGCGGA
ACCGTCGGTGTGCGGTCGCGGTTCGGTGCGGCGGTGCTCGGGTCCCGCACCATACGAGTTGGCGCCTTTCCTATCTCGGT
TGACTCCGGCGCGCTCGACCACGCTGCCCGCGACCGCAACATCAGGCGCCGGGCCCGCGAGATTCGCACCGAACTGGGAA
ATCCGCGCAAGATCCTGCTCGGTGTTGACCGGCTCGACTACACCAAGGGCATCGACGTACGGCTGAAGGCCTTTTCCGAG
CTGCTGGCCGAGGGCCGCGTCAAACGCGACGACACCGTCGTGGTCCAGCTGGCTACCCCGAGCCGCGAGCGGGTGGAGAG
CTACCAGACGCTGCGCAACGACATCGAACGCCAGGTCGGCCACATTAACGGCGAGTACGGTGAGGTTGGCCATCCGGTAG
TGCATTACCTGCATCGACCGGCTCCGCGCGACGAGCTTATCGCTTTCTTCGTGGCCAGCGACGTCATGCTGGTCACCCCA
CTACGCGACGGGATGAACCTGGTGGCCAAGGAGTACGTCGCTTGCCGCAGCGATCTTGGCGGTGCCCTGGTGCTCAGCGA
ATTCACCGGGGCCGCAGCCGAACTCCGGCACGCATACCTGGTCAACCCGCACGACCTGGAAGGCGTCAAGGACGGGATAG
AGGAAGCGCTCAACCAGACGGAGGAGGCGGGCCGGCGGCGAATGCGGTCGCTGCGACGCCAAGTGCTCGCCCACGACGTG
GACCGCTGGGCACAGTCGTTTCTCGACGCTCTCGCCGGGGCACACCCGAGGGGCCAAGGCTAA

Upstream 100 bases:

>100_bases
GCCAGGCCAGGCGAGTCGTCGCCGCATATGCGAACGACGCCGACGAGTGTCGGATCTTCCTGTCCATGCTCGGTATTGGA
CCGGCCAAACTCGAGAGCTA

Downstream 100 bases:

>100_bases
CGGTCAAGCCGCTCCCGCTCGCGAGCAGACGCAGAATCGCCCATTTCGGCACGAAATTGGGCGATTCTGCGTCTGCTCGC
GCCCTGGAAGCTGGTGCGGC

Product: alpha, alpha-trehalose-phosphate synthase [UDP-forming] otsA

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDL
HVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWV
QDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSE
LLAEGRVKRDDTVVVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTP
LRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
DRWAQSFLDALAGAHPRGQG

Sequences:

>Translated_500_residues
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDL
HVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWV
QDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSE
LLAEGRVKRDDTVVVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTP
LRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
DRWAQSFLDALAGAHPRGQG
>Mature_499_residues
APSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDLH
VLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQ
DYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRGT
VGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSEL
LAEGRVKRDDTVVVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPL
RDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDVD
RWAQSFLDALAGAHPRGQG

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=480, Percent_Identity=33.3333333333333, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI115533560, Length=547, Percent_Identity=27.2394881170018, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI115533558, Length=547, Percent_Identity=27.2394881170018, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71987012, Length=559, Percent_Identity=26.1180679785331, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=477, Percent_Identity=36.4779874213836, Blast_Score=313, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6323537, Length=446, Percent_Identity=28.6995515695067, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320279, Length=488, Percent_Identity=30.1229508196721, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323917, Length=454, Percent_Identity=28.1938325991189, Blast_Score=190, Evalue=5e-49,
Organism=Drosophila melanogaster, GI19920676, Length=497, Percent_Identity=35.2112676056338, Blast_Score=278, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 55863; Mature: 55732

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRR
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGAWVGWPGVNDDGAEPDLHVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLY
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEHHHH
HDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQDYQLQLVPKMLRMLRPDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCE
IGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
EEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHCCCCCCC
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILL
CCCHHHHCCHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GVDRLDYTKGIDVRLKAFSELLAEGRVKRDDTVVVQLATPSRERVESYQTLRNDIERQVG
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
HINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPLRDGMNLVAKEYVACRSDLG
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
GALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
CEEEEHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRWAQSFLDALAGAHPRGQG
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
APSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGAWVGWPGVNDDGAEPDLHVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLY
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEHHHH
HDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQDYQLQLVPKMLRMLRPDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCE
IGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
EEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHCCCCCCC
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILL
CCCHHHHCCHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GVDRLDYTKGIDVRLKAFSELLAEGRVKRDDTVVVQLATPSRERVESYQTLRNDIERQVG
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
HINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPLRDGMNLVAKEYVACRSDLG
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
GALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
CEEEEHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRWAQSFLDALAGAHPRGQG
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]