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The map label for this gene is groEL

Identifier: 148824624

GI number: 148824624

Start: 3846564

End: 3848183

Strand: Reverse

Name: groEL

Synonym: TBFG_13451

Alternate gene names: 148824624

Gene position: 3848183-3846564 (Counterclockwise)

Preceding gene: 148824625

Following gene: 148824622

Centisome position: 86.98

GC content: 65.12

Gene sequence:

>1620_bases
ATGAGCAAGCTGATCGAATACGACGAAACCGCGCGTCGCGCCATGGAGGTCGGCATGGACAAGCTGGCCGACACCGTGCG
GGTGACGCTGGGGCCGCGCGGCCGGCATGTGGTGCTGGCCAAGGCGTTTGGCGGACCCACGGTTACCAACGACGGCGTCA
CGGTGGCACGTGAGATCGAGCTGGAAGATCCGTTTGAAGACTTGGGCGCCCAGCTGGTGAAGTCGGTGGCCACCAAGACC
AACGATGTGGCCGGTGACGGCACCACCACCGCAACCATCTTGGCGCAGGCACTGATCAAGGGCGGCCTGAGGCTAGTGGC
CGCCGGCGTCAACCCGATCGCGCTCGGCGTGGGAATCGGCAAGGCCGCCGACGCGGTATCCGAGGCGCTGCTGGCATCGG
CCACGCCGGTGTCCGGCAAGACCGGCATCGCGCAGGTGGCGACGGTGTCCTCGCGCGACGAGCAGATCGGTGACCTGGTT
GGCGAAGCGATGAGCAAGGTCGGCCACGACGGCGTGGTCAGCGTCGAAGAATCCTCGACGCTGGGCACCGAGTTGGAGTT
CACCGAGGGTATCGGCTTCGACAAGGGCTTCTTGTCGGCATACTTCGTTACCGACTTCGATAACCAGCAGGCGGTGCTCG
AGGACGCGTTGATCCTGCTGCACCAAGACAAGATCAGCTCGCTTCCCGATCTGTTGCCATTGCTGGAAAAGGTTGCAGGA
ACGGGTAAGCCACTACTGATCGTGGCTGAAGACGTGGAGGGCGAAGCGTTGGCGACGCTGGTCGTCAACGCGATTCGCAA
GACGTTGAAAGCGGTCGCGGTCAAGGGGCCGTACTTCGGTGACCGCCGTAAGGCGTTCCTTGAGGACCTGGCGGTGGTGA
CGGGTGGCCAGGTGGTCAACCCCGACGCCGGCATGGTGCTGCGCGAGGTGGGCTTGGAGGTGCTGGGCTCGGCCCGACGC
GTGGTGGTCAGCAAGGACGACACGGTCATTGTCGACGGCGGCGGCACCGCAGAAGCGGTGGCCAACCGGGCGAAGCACTT
GCGTGCCGAGATCGACAAGAGCGATTCGGATTGGGATCGGGAAAAGCTTGGCGAGCGGCTGGCCAAACTGGCCGGCGGGG
TTGCTGTCATCAAGGTGGGTGCCGCCACCGAGACCGCACTCAAGGAGCGCAAGGAAAGCGTCGAGGATGCGGTCGCGGCC
GCCAAGGCCGCGGTCGAGGAGGGCATCGTCCCTGGTGGGGGAGCCTCGCTCATCCACCAGGCCCGCAAGGCGCTGACCGA
ACTGCGTGCGTCGCTGACCGGTGACGAGGTCCTCGGTGTCGACGTGTTCTCCGAAGCCCTTGCCGCGCCGTTGTTCTGGA
TCGCCGCCAACGCTGGCTTGGACGGCTCGGTGGTGGTCAACAAGGTCAGCGAGCTACCCGCCGGGCATGGGCTGAACGTG
AACACCCTGAGCTATGGTGACTTGGCCGCTGACGGCGTCATCGACCCGGTCAAGGTGACTAGGTCGGCGGTGTTGAACGC
GTCATCGGTTGCCCGGATGGTACTCACCACCGAGACGGTCGTGGTCGACAAGCCGGCCAAGGCAGAAGATCACGACCATC
ACCACGGGCACGCGCACTGA

Upstream 100 bases:

>100_bases
AAGTAGTAGAGCGTGTTCCGCCCCGGCGATCCCCGTGCTCACCACGGGTGATTTCCGGGGCGGCATGCGTTAGCGGACTA
GCCCTGCGTAGAGGAGCCTG

Downstream 100 bases:

>100_bases
ACTCCGGTCAGCAGACGCAAAAGCCCCCGACACGCCGAGCGTGCGGGGGCTTTAGCGTCTGCTCGCGCGGCTTAAGCTGT
GCGGCGGATGCCGCGGGTGC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60-1

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSKLIEYDETARRAMEVGMDKLADTVRVTLGPRGRHVVLAKAFGGPTVTNDGVTVAREIELEDPFEDLGAQLVKSVATKT
NDVAGDGTTTATILAQALIKGGLRLVAAGVNPIALGVGIGKAADAVSEALLASATPVSGKTGIAQVATVSSRDEQIGDLV
GEAMSKVGHDGVVSVEESSTLGTELEFTEGIGFDKGFLSAYFVTDFDNQQAVLEDALILLHQDKISSLPDLLPLLEKVAG
TGKPLLIVAEDVEGEALATLVVNAIRKTLKAVAVKGPYFGDRRKAFLEDLAVVTGGQVVNPDAGMVLREVGLEVLGSARR
VVVSKDDTVIVDGGGTAEAVANRAKHLRAEIDKSDSDWDREKLGERLAKLAGGVAVIKVGAATETALKERKESVEDAVAA
AKAAVEEGIVPGGGASLIHQARKALTELRASLTGDEVLGVDVFSEALAAPLFWIAANAGLDGSVVVNKVSELPAGHGLNV
NTLSYGDLAADGVIDPVKVTRSAVLNASSVARMVLTTETVVVDKPAKAEDHDHHHGHAH

Sequences:

>Translated_539_residues
MSKLIEYDETARRAMEVGMDKLADTVRVTLGPRGRHVVLAKAFGGPTVTNDGVTVAREIELEDPFEDLGAQLVKSVATKT
NDVAGDGTTTATILAQALIKGGLRLVAAGVNPIALGVGIGKAADAVSEALLASATPVSGKTGIAQVATVSSRDEQIGDLV
GEAMSKVGHDGVVSVEESSTLGTELEFTEGIGFDKGFLSAYFVTDFDNQQAVLEDALILLHQDKISSLPDLLPLLEKVAG
TGKPLLIVAEDVEGEALATLVVNAIRKTLKAVAVKGPYFGDRRKAFLEDLAVVTGGQVVNPDAGMVLREVGLEVLGSARR
VVVSKDDTVIVDGGGTAEAVANRAKHLRAEIDKSDSDWDREKLGERLAKLAGGVAVIKVGAATETALKERKESVEDAVAA
AKAAVEEGIVPGGGASLIHQARKALTELRASLTGDEVLGVDVFSEALAAPLFWIAANAGLDGSVVVNKVSELPAGHGLNV
NTLSYGDLAADGVIDPVKVTRSAVLNASSVARMVLTTETVVVDKPAKAEDHDHHHGHAH
>Mature_538_residues
SKLIEYDETARRAMEVGMDKLADTVRVTLGPRGRHVVLAKAFGGPTVTNDGVTVAREIELEDPFEDLGAQLVKSVATKTN
DVAGDGTTTATILAQALIKGGLRLVAAGVNPIALGVGIGKAADAVSEALLASATPVSGKTGIAQVATVSSRDEQIGDLVG
EAMSKVGHDGVVSVEESSTLGTELEFTEGIGFDKGFLSAYFVTDFDNQQAVLEDALILLHQDKISSLPDLLPLLEKVAGT
GKPLLIVAEDVEGEALATLVVNAIRKTLKAVAVKGPYFGDRRKAFLEDLAVVTGGQVVNPDAGMVLREVGLEVLGSARRV
VVSKDDTVIVDGGGTAEAVANRAKHLRAEIDKSDSDWDREKLGERLAKLAGGVAVIKVGAATETALKERKESVEDAVAAA
KAAVEEGIVPGGGASLIHQARKALTELRASLTGDEVLGVDVFSEALAAPLFWIAANAGLDGSVVVNKVSELPAGHGLNVN
TLSYGDLAADGVIDPVKVTRSAVLNASSVARMVLTTETVVVDKPAKAEDHDHHHGHAH

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=41.8079096045198, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=41.8079096045198, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI38455427, Length=554, Percent_Identity=23.4657039711191, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI24307939, Length=556, Percent_Identity=21.4028776978417, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI5453603, Length=131, Percent_Identity=34.3511450381679, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=525, Percent_Identity=53.5238095238095, Blast_Score=517, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=41.3662239089184, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI193210679, Length=209, Percent_Identity=40.6698564593301, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17564182, Length=523, Percent_Identity=21.7973231357553, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=43.9163498098859, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322524, Length=102, Percent_Identity=38.2352941176471, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=44.6768060836502, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=44.6768060836502, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=43.1558935361217, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=43.1558935361217, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=43.1558935361217, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI17864606, Length=525, Percent_Identity=38.6666666666667, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24584129, Length=526, Percent_Identity=33.2699619771863, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI19921262, Length=526, Percent_Identity=33.2699619771863, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645179, Length=530, Percent_Identity=21.5094339622642, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17647245, Length=553, Percent_Identity=23.5081374321881, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24652903, Length=553, Percent_Identity=23.5081374321881, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH601_MYCBO (P0A519)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857091.1
- ProteinModelPortal:   P0A519
- SMR:   P0A519
- EnsemblBacteria:   EBMYCT00000015053
- GeneID:   1091030
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3451c
- GeneTree:   EBGT00050000016766
- HOGENOM:   HBG625289
- OMA:   WIASNAG
- ProtClustDB:   PRK12849
- BioCyc:   MBOV233413:MB3451C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 55878; Mature: 55747

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIEYDETARRAMEVGMDKLADTVRVTLGPRGRHVVLAKAFGGPTVTNDGVTVAREIE
CCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEEEC
LEDPFEDLGAQLVKSVATKTNDVAGDGTTTATILAQALIKGGLRLVAAGVNPIALGVGIG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCC
KAADAVSEALLASATPVSGKTGIAQVATVSSRDEQIGDLVGEAMSKVGHDGVVSVEESST
HHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
LGTELEFTEGIGFDKGFLSAYFVTDFDNQQAVLEDALILLHQDKISSLPDLLPLLEKVAG
CCCCEEHHCCCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
TGKPLLIVAEDVEGEALATLVVNAIRKTLKAVAVKGPYFGDRRKAFLEDLAVVTGGQVVN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEC
PDAGMVLREVGLEVLGSARRVVVSKDDTVIVDGGGTAEAVANRAKHLRAEIDKSDSDWDR
CCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCH
EKLGERLAKLAGGVAVIKVGAATETALKERKESVEDAVAAAKAAVEEGIVPGGGASLIHQ
HHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ARKALTELRASLTGDEVLGVDVFSEALAAPLFWIAANAGLDGSVVVNKVSELPAGHGLNV
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCCCC
NTLSYGDLAADGVIDPVKVTRSAVLNASSVARMVLTTETVVVDKPAKAEDHDHHHGHAH
CEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHEEECEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLIEYDETARRAMEVGMDKLADTVRVTLGPRGRHVVLAKAFGGPTVTNDGVTVAREIE
CCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEEEC
LEDPFEDLGAQLVKSVATKTNDVAGDGTTTATILAQALIKGGLRLVAAGVNPIALGVGIG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCC
KAADAVSEALLASATPVSGKTGIAQVATVSSRDEQIGDLVGEAMSKVGHDGVVSVEESST
HHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
LGTELEFTEGIGFDKGFLSAYFVTDFDNQQAVLEDALILLHQDKISSLPDLLPLLEKVAG
CCCCEEHHCCCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
TGKPLLIVAEDVEGEALATLVVNAIRKTLKAVAVKGPYFGDRRKAFLEDLAVVTGGQVVN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEC
PDAGMVLREVGLEVLGSARRVVVSKDDTVIVDGGGTAEAVANRAKHLRAEIDKSDSDWDR
CCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCH
EKLGERLAKLAGGVAVIKVGAATETALKERKESVEDAVAAAKAAVEEGIVPGGGASLIHQ
HHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ARKALTELRASLTGDEVLGVDVFSEALAAPLFWIAANAGLDGSVVVNKVSELPAGHGLNV
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCCCC
NTLSYGDLAADGVIDPVKVTRSAVLNASSVARMVLTTETVVVDKPAKAEDHDHHHGHAH
CEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHEEECEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972