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The map label for this gene is amiD [H]

Identifier: 148824584

GI number: 148824584

Start: 3801096

End: 3802523

Strand: Direct

Name: amiD [H]

Synonym: TBFG_13411

Alternate gene names: 148824584

Gene position: 3801096-3802523 (Clockwise)

Preceding gene: 148824583

Following gene: 148824585

Centisome position: 85.91

GC content: 66.32

Gene sequence:

>1428_bases
ATGACCGATGCTGACAGTGCGGTCCCTCCCCGACTCGACGAGGACGCGATCTCGAAACTCGAGCTGACCGAGGTCGCCGA
CCTGATCCGCACCCGGCAACTGACGTCGGCAGAAGTGACCGAGTCGACGCTGCGGCGTATCGAAAGGCTTGACCCCCAGC
TGAAGAGCTACGCCTTCGTCATGCCGGAAACTGCGCTAGCGGCGGCACGTGCCGCCGACTCCGACATCGCGCGCGGCCAC
TACGAGGGTGTCCTGCACGGCGTACCGATCGGCGTGAAGGATCTCTGCTACACGGTCGACGCCCCGACCGCGGCCGGCAC
CACCATCTTTCGTGACTTTCGCCCGGCATACGACGCGACGGTTGTCGCGAGGTTGCGCGCGGCCGGCGCGGTGATCATCG
GCAAGCTGGCCATGACGGAGGGGGCCTATCTCGGCTATCACCCCAGTCTGCCGACCCCGGTCAATCCCTGGGACCCGACA
GCGTGGGCGGGCGTGTCCTCGAGCGGCTGCGGCGTGGCCACCGCGGCGGGATTGTGCTTCGGCTCGATCGGGTCGGACAC
CGGGGGGTCGATTCGCTTTCCGACGAGCATGTGCGGCGTCACCGGGATCAAACCGACGTGGGGCCGGGTCAGCCGTCACG
GCGTCGTCGAACTTGCGGCAAGCTACGACCACGTCGGGCCGATCACCCGTAGCGCTCACGATGCGGCGGTATTGCTCAGT
GTCATAGCGGGATCCGATATCCACGATCCCTCGTGCTCGGCGGAGCCCGTTCCGGACTATGCCGCCGACCTCGCCTTGAC
ACGGATTCCGCGTGTCGGGGTGGACTGGTCGCAGACGACGTCGTTTGACGAGGACACCACGGCGATGCTGGCCGATGTCG
TCAAAACGCTCGACGACATCGGATGGCCCGTCATCGACGTCAAGCTGCCCGCGCTTGCGCCGATGGTGGCAGCGTTCGGA
AAAATGCGCGCGGTCGAAACGGCGATCGCGCATGCCGACACCTACCCGGCGCGCGCCGACGAGTACGGGCCGATCATGCG
CGCAATGATCGACGCCGGACACAGGCTGGCTGCGGTGGAATATCAGACGCTGACCGAGCGGCGTCTGGAATTCACGCGAT
CGCTGCGTCGCGTGTTCCACGACGTGGACATCCTGCTGATGCCCAGCGCCGGAATTGCCTCGCCCACACTGGAAACCATG
CGCGGGCTCGGACAAGACCCGGAGCTGACCGCCAGACTGGCGATGCCGACAGCACCGTTCAACGTCAGCGGTAATCCCGC
GATATGCCTACCGGCGGGAACGACGGCGCGCGGAACGCCGCTCGGCGTCCAGTTCATCGGCCGTGAATTCGACGAGCACT
TGCTCGTCCGAGCCGGCCACGCATTTCAGCAAGTCACCGGGTATCATCGCCGACGCCCGCCGGTGTGA

Upstream 100 bases:

>100_bases
CTGATCCGGTTCGGTGTCGAAGCCTACGAGGGGGCGGACCTACGCGAAGGGGTGGCGGCCTTCAGCCAGGGACGCCCACC
CAAATTTGATGATTAGCGCC

Downstream 100 bases:

>100_bases
AAAACCCTCGGCCGCAAAAGGCTTGCGAATGTCGCACCGAAGGTCGCGGCGAATCGCCTTACTGGTATGTTTACGAACAC
AATCTGTGGCCATCAAGGGA

Product: amidase amiD (acylamidase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADSDIARGH
YEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPT
AWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFG
KMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETM
RGLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV

Sequences:

>Translated_475_residues
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADSDIARGH
YEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPT
AWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFG
KMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETM
RGLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
>Mature_474_residues
TDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADSDIARGHY
EGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTA
WAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSV
IAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFGK
MRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMR
GLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=28.6290322580645, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=26.6401590457256, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI166795287, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=442, Percent_Identity=27.6018099547511, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=296, Percent_Identity=24.6621621621622, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=305, Percent_Identity=25.2459016393443, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=401, Percent_Identity=26.1845386533666, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=255, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=469, Percent_Identity=31.5565031982942, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI161078093, Length=496, Percent_Identity=25.8064516129032, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=25.1461988304094, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=26.6932270916335, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=26.6932270916335, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=26.6932270916335, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=26.6932270916335, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=23.265306122449, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=30.2127659574468, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=30.2127659574468, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=30.2127659574468, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50662; Mature: 50531

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEEEE
MPETALAAARAADSDIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDAT
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCHHHHHHCCCHHHHH
VVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCF
HHHHHHHCCHHEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
HCCCCCCCCCEECCHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDI
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GWPVIDVKLPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVE
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEHH
YQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPF
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCC
NVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEEEE
MPETALAAARAADSDIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDAT
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCHHHHHHCCCHHHHH
VVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCF
HHHHHHHCCHHEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
HCCCCCCCCCEECCHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDI
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GWPVIDVKLPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVE
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEHH
YQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPF
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCC
NVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]