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The map label for this gene is purK

Identifier: 148824479

GI number: 148824479

Start: 3670413

End: 3671702

Strand: Reverse

Name: purK

Synonym: TBFG_13305

Alternate gene names: 148824479

Gene position: 3671702-3670413 (Counterclockwise)

Preceding gene: 148824480

Following gene: 148824477

Centisome position: 82.99

GC content: 68.53

Gene sequence:

>1290_bases
ATGATGGCCGTGGCGAGCTCACGCACCCCGGCGGTCACCTCGTTCATTGCCCCCCTCGTTGCGATGGTCGGTGGCGGTCA
GCTGGCCCGGATGACCCATCAGGCTGCCATCGCGCTGGGGCAGAACCTCCGTGTATTGGTCACCTCGGCAGATGACCCTG
CCGCGCAGGTCACCCCCAACGTGGTGATCGGCTCGCACACCGATCTTGCCGCCTTGCGCCGCGTTGCGGCCGGCGCCGAC
GTGCTGACCTTTGACCACGAGCACGTCCCGAACGAGCTGTTGGAGAAGCTAGTCGCCGATGGCGTCAATGTCGCACCGTC
GCCGCAGGCGCTGGTGCACGCCCAGGACAAACTCGTCATGCGGCAGCGTCTGGCTGCAGCAGGCGTCGCCGTGCCCCGCT
ACGCGGGCATCAAAGACCCCGACGAAATCGACGTCTTCGCCGCACGTGTCGACGCCCCGATCGTGGTCAAGGCAGTGCGC
GGGGGTTACGACGGCCGGGGCGTTCGGATGGCACGTGATGTGGCGGATGCCCGTGATTTCGCTCGCGAATGCCTGGCTGA
CGGCGTGGCGGTGCTGGTCGAGGAACGGGTGGACCTGCGCCGCGAGCTGTCGGCACTGGTGGCGCGTTCGCCGTTCGGCC
AGGGTGCGGCGTGGCCGGTGGTCCAGACGGTGCAACGTGACGGCACGTGTGTGCTGGTGATCGCGCCGGCGCCGGCGCTG
CCCGACGACCTGGCCACCGCGGCGCAACGGTTGGCGTTGCAGCTGGCCGACGAACTCGGTGTGGTCGGCGTGCTCGCCGT
CGAGCTGTTCGAGACGACCGACGGGGCGTTGCTGGTCAACGAGCTCGCGATGCGGCCACATAATTCCGGGCACTGGACCA
TCGACGGGGCGCGCACCAGCCAGTTCGAGCAGCATCTGCGCGCGGTCTTGGACTACCCGCTCGGCGACAGCGACGCCGTG
GTGCCCGTGACGGTGATGGCCAATGTGCTCGGCGCCGCGCAGCCGCCGGCCATGAGCGTCGACGAGCGGCTGCACCATCT
GTTTGCGCGAATGCCCGACGCCCGGGTTCACCTCTACGGCAAGGCCGAGCGACCCGGTCGCAAGGTGGGGCATATCAACT
TCCTTGGCTCCGATGTCGCCCAGTTGTGTGAACGCGCTGAGCTGGCGGCACACTGGTTGTCCCATGGGCGGTGGACTGAC
GGATGGGACCCACACCGCGCCAGCGACGATGCAGTGGGGGTACCACCCGCGTGCGGGGGACGAAGCGATGAGGAGGAGCG
GCGCCTATGA

Upstream 100 bases:

>100_bases
AACAAGTGAGTGTACGGACGAACACAAAATCGACGCAAAATGATACGTAACGGTTTGGCTGCCGTGGCCGGCAAGTACGC
CGGTCAGATGCCGTGACACC

Downstream 100 bases:

>100_bases
CTCCTGCCGGCGAGCGGCCCCGGGTCGGGGTGATCATGGGCAGCGACAGCGACTGGCCGGTGATGGCCGACGCTGCGGCG
GCGCTGGCCGAGTTCGACAT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRVLVTSADDPAAQVTPNVVIGSHTDLAALRRVAAGAD
VLTFDHEHVPNELLEKLVADGVNVAPSPQALVHAQDKLVMRQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVR
GGYDGRGVRMARDVADARDFARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQTVQRDGTCVLVIAPAPAL
PDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNELAMRPHNSGHWTIDGARTSQFEQHLRAVLDYPLGDSDAV
VPVTVMANVLGAAQPPAMSVDERLHHLFARMPDARVHLYGKAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWTD
GWDPHRASDDAVGVPPACGGRSDEEERRL

Sequences:

>Translated_429_residues
MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRVLVTSADDPAAQVTPNVVIGSHTDLAALRRVAAGAD
VLTFDHEHVPNELLEKLVADGVNVAPSPQALVHAQDKLVMRQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVR
GGYDGRGVRMARDVADARDFARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQTVQRDGTCVLVIAPAPAL
PDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNELAMRPHNSGHWTIDGARTSQFEQHLRAVLDYPLGDSDAV
VPVTVMANVLGAAQPPAMSVDERLHHLFARMPDARVHLYGKAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWTD
GWDPHRASDDAVGVPPACGGRSDEEERRL
>Mature_429_residues
MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRVLVTSADDPAAQVTPNVVIGSHTDLAALRRVAAGAD
VLTFDHEHVPNELLEKLVADGVNVAPSPQALVHAQDKLVMRQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVR
GGYDGRGVRMARDVADARDFARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQTVQRDGTCVLVIAPAPAL
PDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNELAMRPHNSGHWTIDGARTSQFEQHLRAVLDYPLGDSDAV
VPVTVMANVLGAAQPPAMSVDERLHHLFARMPDARVHLYGKAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWTD
GWDPHRASDDAVGVPPACGGRSDEEERRL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=369, Percent_Identity=31.4363143631436, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788155, Length=400, Percent_Identity=27.25, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=385, Percent_Identity=33.5064935064935, Blast_Score=198, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_MYCBO (P65899)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856949.1
- ProteinModelPortal:   P65899
- SMR:   P65899
- EnsemblBacteria:   EBMYCT00000016845
- GeneID:   1093915
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3304c
- GeneTree:   EBGT00050000015722
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- ProtClustDB:   PRK06019
- BioCyc:   MBOV233413:MB3304C-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 45696; Mature: 45696

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 117-117 BINDING 157-157 BINDING 202-202

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRVLVTSADDPAAQVTPN
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCC
VVIGSHTDLAALRRVAAGADVLTFDHEHVPNELLEKLVADGVNVAPSPQALVHAQDKLVM
EEECCCCHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVRGGYDGRGVRMARDVADARDF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHH
ARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQTVQRDGTCVLVIAPAPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCC
PDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNELAMRPHNSGHWTIDGARTS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCCHHH
QFEQHLRAVLDYPLGDSDAVVPVTVMANVLGAAQPPAMSVDERLHHLFARMPDARVHLYG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
KAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWTDGWDPHRASDDAVGVPPACGG
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RSDEEERRL
CCCHHHCCC
>Mature Secondary Structure
MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRVLVTSADDPAAQVTPN
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCC
VVIGSHTDLAALRRVAAGADVLTFDHEHVPNELLEKLVADGVNVAPSPQALVHAQDKLVM
EEECCCCHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVRGGYDGRGVRMARDVADARDF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHH
ARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQTVQRDGTCVLVIAPAPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCC
PDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNELAMRPHNSGHWTIDGARTS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCCHHH
QFEQHLRAVLDYPLGDSDAVVPVTVMANVLGAAQPPAMSVDERLHHLFARMPDARVHLYG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
KAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWTDGWDPHRASDDAVGVPPACGG
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RSDEEERRL
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972