BacMap

Definition	Mycobacterium tuberculosis F11, complete genome.
Accession	NC_009565
Length	4,424,435

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The map label for this gene is fadE25

Identifier: 148824476

GI number: 148824476

Start: 3668698

End: 3669867

Strand: Reverse

Name: fadE25

Synonym: TBFG_13303

Alternate gene names: 148824476

Gene position: 3669867-3668698 (Counterclockwise)

Preceding gene: 148824477

Following gene: 148824473

Centisome position: 82.95

GC content: 64.19

Gene sequence:

>1170_bases
ATGGTTGGATGGGCCGGAAACCCGTCGTTCGATCTGTTCAAGCTGCCCGAGGAACACGACGAAATGCGATCGGCGATACG
GGCTTTGGCGGAAAAGGAGATCGCTCCGCATGCCGCCGAGGTGGACGAGAAGGCGCGGTTCCCCGAAGAAGCGCTAGTGG
CGCTCAATTCCTCCGGTTTCAACGCCGTCCACATTCCGGAGGAGTACGGCGGTCAGGGTGCCGACTCGGTAGCTACCTGC
ATCGTGATCGAAGAGGTGGCCCGCGTCGACGCGTCCGCGTCGTTGATCCCCGCGGTCAACAAGCTGGGCACCATGGGCCT
GATCCTGCGGGGCTCCGAGGAGCTGAAGAAGCAGGTGCTGCCGGCGTTGGCCGCCGAAGGGGCGATGGCTTCCTATGCAT
TGAGTGAACGCGAGGCCGGCAGTGACGCGGCGTCCATGCGGACGCGGGCCAAGGCCGATGGGGATCACTGGATTCTCAAC
GGCGCCAAGTGCTGGATCACCAACGGCGGCAAGTCGACCTGGTACACGGTGATGGCGGTGACCGATCCCGACCGGGGCGC
CAACGGCATCTCGGCGTTCATGGTGCACAAGGACGACGAGGGGTTCACCGTCGGTCCGAAAGAACGCAAGCTCGGGATCA
AGGGGTCACCGACCACCGAGCTGTACTTCGAGAACTGCCGCATCCCCGGCGATCGCATCATCGGTGAGCCCGGTACCGGT
TTCAAGACCGCGCTGGCCACGTTGGACCACACCCGTCCCACGATTGGCGCGCAGGCCGTGGGTATCGCCCAGGGCGCGCT
GGATGCTGCCATCGCCTACACCAAGGACCGCAAGCAGTTCGGTGAGTCGATCAGTACGTTTCAGGCCGTGCAGTTCATGC
TGGCCGACATGGCGATGAAGGTGGAGGCGGCTCGGCTGATGGTTTACTCCGCCGCCGCCCGCGCCGAACGCGGTGAGCCG
GATCTGGGCTTCATTTCGGCGGCCTCGAAGTGCTTCGCCTCCGACGTCGCGATGGAGGTCACCACCGATGCCGTACAACT
GTTCGGCGGCGCCGGCTACACCACCGACTTCCCGGTCGAGCGGTTTATGCGCGACGCCAAGATCACCCAGATCTATGAGG
GCACCAATCAGATTCAGCGGGTAGTGATGTCGCGGGCGCTACTGCGCTGA

Upstream 100 bases:

>100_bases
GGACCGGCTGGCCGACGTCGTGGCGGCCAAGGATGCGGAACTGCAGCGGCTTGCGGGTAAGTTAACCCGCGACTAGCACG
GAGATTAGGAGGGCTCCAAG

Downstream 100 bases:

>100_bases
TTCGTGGTCGACGGGGCGGTCGGCCGGCTCGTCCGGGCAGATGATGCCGTTCGGACCCACCTCGTATACCCGGGCGGTCG
AGATGTCGAAGAATATGCCG

Product: acyl-CoA dehydrogenase fadE25

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MVGWAGNPSFDLFKLPEEHDEMRSAIRALAEKEIAPHAAEVDEKARFPEEALVALNSSGFNAVHIPEEYGGQGADSVATC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPALAAEGAMASYALSEREAGSDAASMRTRAKADGDHWILN
GAKCWITNGGKSTWYTVMAVTDPDRGANGISAFMVHKDDEGFTVGPKERKLGIKGSPTTELYFENCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIAYTKDRKQFGESISTFQAVQFMLADMAMKVEAARLMVYSAAARAERGEP
DLGFISAASKCFASDVAMEVTTDAVQLFGGAGYTTDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR

Sequences:

>Translated_389_residues
MVGWAGNPSFDLFKLPEEHDEMRSAIRALAEKEIAPHAAEVDEKARFPEEALVALNSSGFNAVHIPEEYGGQGADSVATC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPALAAEGAMASYALSEREAGSDAASMRTRAKADGDHWILN
GAKCWITNGGKSTWYTVMAVTDPDRGANGISAFMVHKDDEGFTVGPKERKLGIKGSPTTELYFENCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIAYTKDRKQFGESISTFQAVQFMLADMAMKVEAARLMVYSAAARAERGEP
DLGFISAASKCFASDVAMEVTTDAVQLFGGAGYTTDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR
>Mature_389_residues
MVGWAGNPSFDLFKLPEEHDEMRSAIRALAEKEIAPHAAEVDEKARFPEEALVALNSSGFNAVHIPEEYGGQGADSVATC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPALAAEGAMASYALSEREAGSDAASMRTRAKADGDHWILN
GAKCWITNGGKSTWYTVMAVTDPDRGANGISAFMVHKDDEGFTVGPKERKLGIKGSPTTELYFENCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIAYTKDRKQFGESISTFQAVQFMLADMAMKVEAARLMVYSAAARAERGEP
DLGFISAASKCFASDVAMEVTTDAVQLFGGAGYTTDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4557233, Length=383, Percent_Identity=44.6475195822454, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI4557231, Length=376, Percent_Identity=41.2234042553192, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI187960098, Length=376, Percent_Identity=41.2234042553192, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4501859, Length=374, Percent_Identity=39.3048128342246, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI21361497, Length=387, Percent_Identity=37.984496124031, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI7656849, Length=375, Percent_Identity=36, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI226958412, Length=383, Percent_Identity=35.2480417754569, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI226958414, Length=341, Percent_Identity=36.3636363636364, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4557235, Length=338, Percent_Identity=36.9822485207101, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI76496475, Length=338, Percent_Identity=36.9822485207101, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI4501857, Length=373, Percent_Identity=32.4396782841823, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4503943, Length=373, Percent_Identity=24.9329758713137, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI7669494, Length=359, Percent_Identity=24.5125348189415, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI211971008, Length=295, Percent_Identity=27.1186440677966, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI48976061, Length=295, Percent_Identity=27.1186440677966, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI38505218, Length=301, Percent_Identity=22.5913621262458, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786223, Length=381, Percent_Identity=29.9212598425197, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87081958, Length=380, Percent_Identity=30.2631578947368, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081702, Length=375, Percent_Identity=26.4, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87082384, Length=322, Percent_Identity=22.6708074534161, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17570075, Length=379, Percent_Identity=42.2163588390501, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17569725, Length=379, Percent_Identity=40.8970976253298, Blast_Score=300, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI17508101, Length=371, Percent_Identity=41.2398921832884, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17534899, Length=380, Percent_Identity=40.2631578947368, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17506239, Length=363, Percent_Identity=39.6694214876033, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17533517, Length=375, Percent_Identity=36.5333333333333, Blast_Score=261, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17538396, Length=379, Percent_Identity=36.4116094986807, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71990804, Length=280, Percent_Identity=41.4285714285714, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71985184, Length=351, Percent_Identity=35.6125356125356, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI32563615, Length=229, Percent_Identity=42.7947598253275, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71982178, Length=279, Percent_Identity=34.4086021505376, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI71985192, Length=285, Percent_Identity=36.140350877193, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17551932, Length=373, Percent_Identity=29.7587131367292, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17505929, Length=377, Percent_Identity=29.1777188328912, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531909, Length=236, Percent_Identity=34.7457627118644, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17534353, Length=378, Percent_Identity=25.3968253968254, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531913, Length=171, Percent_Identity=32.1637426900585, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI86563381, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI86563383, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562446, Length=349, Percent_Identity=26.647564469914, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24660351, Length=384, Percent_Identity=40.3645833333333, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI21356377, Length=379, Percent_Identity=40.8970976253298, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24646207, Length=378, Percent_Identity=39.4179894179894, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24666513, Length=376, Percent_Identity=38.2978723404255, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281363737, Length=355, Percent_Identity=36.056338028169, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21355753, Length=385, Percent_Identity=34.5454545454545, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI19920834, Length=380, Percent_Identity=27.6315789473684, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI221473076, Length=324, Percent_Identity=26.8518518518519, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550141, Length=324, Percent_Identity=26.8518518518519, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACDP_MYCBO (P63428)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856947.1
- ProteinModelPortal:   P63428
- SMR:   P63428
- EnsemblBacteria:   EBMYCT00000016201
- GeneID:   1093887
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3302c
- GeneTree:   EBGT00050000015288
- HOGENOM:   HBG699365
- OMA:   DENARFP
- ProtClustDB:   CLSK872163
- BioCyc:   MBOV233413:MB3302C-MONOMER
- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100
- Gene3D:   G3DSA:2.40.110.10
- Gene3D:   G3DSA:1.10.540.10
- Gene3D:   G3DSA:1.20.140.10

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N; SSF56645 AcylCoA_dehyd_NM; SSF47203 AcylCoADH_C_like

EC number: 1.3.99.- [C]

Molecular weight: Translated: 41724; Mature: 41724

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00072 ACYL_COA_DH_1; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGWAGNPSFDLFKLPEEHDEMRSAIRALAEKEIAPHAAEVDEKARFPEEALVALNSSGF
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEECCCCC
NAVHIPEEYGGQGADSVATCIVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVL
CEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHH
PALAAEGAMASYALSEREAGSDAASMRTRAKADGDHWILNGAKCWITNGGKSTWYTVMAV
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECEEEEEECCCCCEEEEEEEE
TDPDRGANGISAFMVHKDDEGFTVGPKERKLGIKGSPTTELYFENCRIPGDRIIGEPGTG
ECCCCCCCCEEEEEEEECCCCCEECCHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCC
FKTALATLDHTRPTIGAQAVGIAQGALDAAIAYTKDRKQFGESISTFQAVQFMLADMAMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAARLMVYSAAARAERGEPDLGFISAASKCFASDVAMEVTTDAVQLFGGAGYTTDFPVE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RFMRDAKITQIYEGTNQIQRVVMSRALLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVGWAGNPSFDLFKLPEEHDEMRSAIRALAEKEIAPHAAEVDEKARFPEEALVALNSSGF
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEECCCCC
NAVHIPEEYGGQGADSVATCIVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVL
CEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHH
PALAAEGAMASYALSEREAGSDAASMRTRAKADGDHWILNGAKCWITNGGKSTWYTVMAV
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECEEEEEECCCCCEEEEEEEE
TDPDRGANGISAFMVHKDDEGFTVGPKERKLGIKGSPTTELYFENCRIPGDRIIGEPGTG
ECCCCCCCCEEEEEEEECCCCCEECCHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCC
FKTALATLDHTRPTIGAQAVGIAQGALDAAIAYTKDRKQFGESISTFQAVQFMLADMAMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAARLMVYSAAARAERGEPDLGFISAASKCFASDVAMEVTTDAVQLFGGAGYTTDFPVE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RFMRDAKITQIYEGTNQIQRVVMSRALLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972