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The map label for this gene is rmlD

Identifier: 148824468

GI number: 148824468

Start: 3658673

End: 3659587

Strand: Reverse

Name: rmlD

Synonym: TBFG_13295

Alternate gene names: 148824468

Gene position: 3659587-3658673 (Counterclockwise)

Preceding gene: 148824473

Following gene: 148824467

Centisome position: 82.71

GC content: 69.07

Gene sequence:

>915_bases
ATGGCGGGCAGGTCAGAAAGGCTAGTCATCACCGGTGCGGGCGGGCAGCTGGGCAGCCATCTGACGGCACAGGCCGCCCG
GGAAGGCCGCGATATGCTGGCGCTGACTTCCTCGCAGTGGGATATCACCGACCCCGCTGCGGCCGAGCGGATCATCCGAC
ACGGTGACGTGGTGATCAATTGCGCCGCCTACACCGACGTCGACGGCGCCGAGAGCAATGAGGCAGTCGCCTATGCGGTC
AATGCCACTGGCCCGCAACACCTCGCCCGTGCGTGCGCTCGTGTGGGTGCTCGGTTAATCCACGTCTCCACCGACTACGT
ATTCGATGGCGACTTCGGCGGCGCCGAGCCCCGGCCCTACGAGCCCACCGATGAAACCGCGCCGCAGGGCGTGTATGCCC
GCAGCAAACTCGCCGGCGAGCAGGCTGTGCTGGCAGCGTTCCCGGAGGCTGCCGTGGTACGGACCGCCTGGGTCTACACC
GGCGGGACCGGCAAGGATTTCGTCGCCGTCATGCGCCGGCTGGCCGCCGGACACGGTCGGGTGGATGTGGTCGACGACCA
GACCGGGTCGCCGACCTACGTCGCCGACCTGGCCGAGGCGCTGCTGGCGTTGGCCGACGCGGGCGTGCGCGGACGCGTGT
TGCACGCCGCTAACGAGGGCGTGGTCTCCCGGTTCGGTCAAGCCCGCGCGGTGTTCGAAGAATGCGGTGCCGACCCACAG
CGGGTGCGTCCGGTCAGCAGCGCGCAGTTTCCGCGGCCCGCGCCGCGTTCGAGCTATTCCGCGCTGTCGAGCAGGCAGTG
GGCGCTGGCGGGCTTGACGCCGCTACGGCACTGGCGCAGCGCACTGGCCACGGCGCTGGCAGCACCTGCGAACTCAACTT
CGATCGATCGACGGTTACCCTCAACGCGTGACTGA

Upstream 100 bases:

>100_bases
GAACCACACGTTGCGCAGACATCACACTAGACTACTTGTGTAACGGCGCCCTGTCGGGTAGCCAACCCCGGCGTGCCCGA
GGCGTGCCAGACTCAAACAC

Downstream 100 bases:

>100_bases
CGTCCTGCCGGTGGTAGCGGTGACCTACTCGCCGGGGCCCCATCTAGAGCGGTTCCTGGCGTCGTTGTCGCTGGCCACCG
AGCGGCCGGTCAGCGTGCTG

Product: dTDP-6-deoxy-L-lyxo-4-hexulose reductase rmlD

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVINCAAYTDVDGAESNEAVAYAV
NATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPYEPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYT
GGTGKDFVAVMRRLAAGHGRVDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQ
RVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLPSTRD

Sequences:

>Translated_304_residues
MAGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVINCAAYTDVDGAESNEAVAYAV
NATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPYEPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYT
GGTGKDFVAVMRRLAAGHGRVDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQ
RVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLPSTRD
>Mature_303_residues
AGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVINCAAYTDVDGAESNEAVAYAVN
ATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPYEPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYTG
GTGKDFVAVMRRLAAGHGRVDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQR
VRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLPSTRD

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galacta

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family

Homologues:

Organism=Homo sapiens, GI33519455, Length=272, Percent_Identity=28.6764705882353, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI11034825, Length=272, Percent_Identity=28.6764705882353, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788352, Length=305, Percent_Identity=32.1311475409836, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLD_MYCTU (P96871)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   C70978
- RefSeq:   NP_217783.1
- RefSeq:   NP_337893.1
- HSSP:   P26392
- ProteinModelPortal:   P96871
- SMR:   P96871
- EnsemblBacteria:   EBMYCT00000002125
- EnsemblBacteria:   EBMYCT00000071952
- GeneID:   888704
- GeneID:   922434
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3366
- KEGG:   mtu:Rv3266c
- TIGR:   MT3366
- TubercuList:   Rv3266c
- GeneTree:   EBGT00050000015199
- HOGENOM:   HBG541114
- OMA:   RPWRDAL
- ProtClustDB:   CLSK792390
- GO:   GO:0005488
- InterPro:   IPR005913
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01214

Pfam domain/function: PF04321 RmlD_sub_bind

EC number: =1.1.1.133

Molecular weight: Translated: 32045; Mature: 31914

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: BINDING 157-157 BINDING 158-158 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVIN
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
CAAYTDVDGAESNEAVAYAVNATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPY
EEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCC
EPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYTGGTGKDFVAVMRRLAAGHGR
CCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHEEEEEEEECCCCHHHHHHHHHHHCCCCC
VDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQ
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHH
RVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLP
HCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
STRD
CCCC
>Mature Secondary Structure 
AGRSERLVITGAGGQLGSHLTAQAAREGRDMLALTSSQWDITDPAAAERIIRHGDVVIN
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
CAAYTDVDGAESNEAVAYAVNATGPQHLARACARVGARLIHVSTDYVFDGDFGGAEPRPY
EEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCC
EPTDETAPQGVYARSKLAGEQAVLAAFPEAAVVRTAWVYTGGTGKDFVAVMRRLAAGHGR
CCCCCCCCCCHHHHHHHCCCHHHHHHCCCHHHEEEEEEEECCCCHHHHHHHHHHHCCCCC
VDVVDDQTGSPTYVADLAEALLALADAGVRGRVLHAANEGVVSRFGQARAVFEECGADPQ
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHH
RVRPVSSAQFPRPAPRSSYSALSSRQWALAGLTPLRHWRSALATALAAPANSTSIDRRLP
HCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
STRD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036