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The map label for this gene is tgs3 [H]

Identifier: 148824436

GI number: 148824436

Start: 3622152

End: 3622967

Strand: Reverse

Name: tgs3 [H]

Synonym: TBFG_13263

Alternate gene names: 148824436

Gene position: 3622967-3622152 (Counterclockwise)

Preceding gene: 148824438

Following gene: 148824435

Centisome position: 81.89

GC content: 64.22

Gene sequence:

>816_bases
GTGGTCACCCGGTTGTCTGCATCGGACGCCTCCTTCTATCAGCTGGAGAACACAGCCACCCCAATGTATGTCGGGTTGCT
GTTGATCCTGCGCCGTCCGCGTGCCGGATTGAGCTACGAGGCACTGCTGGAAACCGTCGAACAGCGGCTGCCGCAGATAC
CGCGCTATCGTCAGAAAGTCCAGGAAGTGAAATTGGGCCTGGCCAGGCCGGTGTGGATCGACGATCGCGACTTCGACATC
ACCTACCACGTCCGGCGGTCGGCCCTGCCGTCGCCGGGTAGTGACGAGCAACTGCACGAGCTGATCGCACGGCTGGCCGC
TCGGCCGCTGGACAAATCGCGGCCATTGTGGGAGATGTATCTCGTCGAGGGTCTGGAAAAGAATCGCATTGCCCTCTACA
CCAAGTCGCACCAAGCCCTGATCAACGGGGTGACCGCGCTGGCGATAGGCCACGTGATCGCCGACCGGACGCGGCGTCCA
CCGGCGTTTCCGGAAGACATCTGGGTCCCGGAACGGGACCCCGGCACCACGCGGTTGCTGCTGCGCGCGGTCGGGGACTG
GCTGGTGCGGCCGGGCGCACAACTACAGGCCGTCGGATCCGCGGTCGCCGGGTTGGTAACGAATTCCGGCCAACTCGTCG
AGACGGGCCGCAAGGTTCTCGATATCGCCCGCACGGTGGCACGGGGCACCGCGCCCAGTAGCCCGCTCAATGCCACCGTG
TCGCGTAATCGGCGATTTACGGTTGCCCATGCAAGTCTTGATGACTATCGCACGGTGCGGGCTCGCTACGACTGTGACTC
CACGACGTGGTGCTGA

Upstream 100 bases:

>100_bases
GGGCTTGCGTGTACCCGCGCCGTGAAGTGGTCGGGCTAACCGTTAGCGTGGGCGACGACGTGACTCGTCGCGACGAATTG
ACAAGGAGGACAGGGCCGTA

Downstream 100 bases:

>100_bases
CGGTGATTGCCGGTGCCTTGGGCAACTGGCTGATGTCACGCGGTGAAGCTGTGGCACCGACCGCGACTGTTCGGGCGATG
GCGCCGCTGTCGGTCTATGC

Product: hypothetical protein

Products: NA

Alternate protein names: TGS3; Probable triacylglycerol synthase tgs3 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVAHASLDDYRTVRARYDCDSTTWC

Sequences:

>Translated_271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVAHASLDDYRTVRARYDCDSTTWC
>Mature_271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVAHASLDDYRTVRARYDCDSTTWC

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 30360; Mature: 30360

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMY
HHHHHCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLL
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SRNRRFTVAHASLDDYRTVRARYDCDSTTWC
CCCCEEEEEECCCHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMY
HHHHHCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLL
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SRNRRFTVAHASLDDYRTVRARYDCDSTTWC
CCCCEEEEEECCCHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]