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The map label for this gene is uvrD

Identifier: 148824393

GI number: 148824393

Start: 3580874

End: 3582976

Strand: Reverse

Name: uvrD

Synonym: TBFG_13220

Alternate gene names: 148824393

Gene position: 3582976-3580874 (Counterclockwise)

Preceding gene: 148824395

Following gene: 148824392

Centisome position: 80.98

GC content: 67.43

Gene sequence:

>2103_bases
ATGTCGATAGCCAGCGACCCATTGATTGCCGGGCTGGACGACCAGCAGCGCGAAGCCGTACTGGCTCCGCGTGGACCGGT
GTGCGTGCTGGCCGGTGCCGGAACCGGCAAGACCCGCACCATCACTCACCGGATCGCTTCGCTGGTCGCGAGCGGTCACG
TCGCCGCCGGGCAGGTGCTGGCTGTGACATTCACCCAGCGCGCGGCGGGCGAAATGCGCAGCCGGTTGCGCGCACTCGAC
GCCGCGGCACGGACCGGTTCGGGCGTCGGCGCTGTGCAGGCGCTGACCTTTCACGCGGCCGCGTATCGCCAGCTGCGGTA
CTTCTGGTCGCGCGTAATCGCTGACACCGGCTGGCAGCTGCTGGACAGCAAGTTCGCCGTGGTGGCCCGCGCCGCCAGCC
GCACCAGGCTGCACGCCAGCACCGATGATGTGCGTGACCTGGCCGGCGAGATCGAGTGGGCCAAGGCGTCCCTGATCGGC
CCCGAGGAGTATGTGACCGCAGTGGCGGCCGCACGCCGAGACCCCCCGTTGGACGCCGCGCAAATCGCGGCTGTCTACTC
CGAATACGAGGCGCTCAAGGCCCGCGGCGACGGCGTCACCTTACTTGACTTCGACGACCTGTTGCTGCACACCGCGGCCG
CGATTGAAAACGACGCCGCGGTGGCCGAGGAGTTCCAGGACCGGTACCGGTGTTTCGTCGTGGACGAGTACCAGGACGTC
ACCCCCCTGCAGCAGCGGGTGCTCTCGGCCTGGTTGGGTGATCGGGACGACCTGACCGTCGTCGGCGACGCCAACCAGAC
CATCTACTCGTTTACCGGGGCTTCGCCTCGTTTCCTACTCGACTTCTCGCGGCGGTTCCCGGACGCCGCGGTGGTGCGGC
TGGAGCGTGATTACCGGTCAACTCCGCAGGTGGTGTCATTGGCTAACCGGGTGATCGCCGCCGCCCGGGGCCGTGTTGCC
GGCAGCAAGCTACGGTTGTCCGGCCAGCGTGAGCCGGGTCCGGTCCCGTCGTTCCATGAGCATTCCGACGAGCCCGCCGA
GGCTGCCACGGTGGCGGCGTCAATCGCCCGGTTAATCGCATCGGGCACTCCGCCATCCGAGGTAGCCATTCTCTACCGAG
TCAATGCGCAGTCAGAGGTTTATGAAGAGGCGTTGACCCAGGCGGGTATCGCCTATCAGGTTCGCGGTGGCGAGGGGTTT
TTCAACCGTCAGGAAATCAAGCAGGCGTTGCTTGCCTTACAGCGTGTGTCCGAACGTGATACCGATGCCGCGTTGTCCGA
CGTGGTTCGCGCGGTGCTCGCACCACTGGGGCTGACGGCACAACCGCCGGTCGGCACCCGCGCCAGGGAACGCTGGGAAG
CGCTCACCGCGCTGGCCGAGCTGGTCGACGACGAGCTGGCGCAGCGTCCCGCACTGCAGCTGCCAGGCCTGCTTGCCGAG
CTACGCCGGCGGGCGGAGGCGCGGCACCCACCGGTGGTGCAGGGCGTCACGCTGGCCTCACTGCACGCCGCCAAGGGACT
GGAATGGGACGCGGTGTTCCTGGTCGGATTGGCTGACGGCACGCTGCCCATCTCACATGCGCTGGCACACGGCCCCAATA
GCGAGCCCGTAGAGGAAGAGCGTCGGCTGCTCTACGTCGGAATCACACGGGCCCGAGTCCATTTGGCGCTCAGCTGGGCG
CTATCACGGTCCCCGGGCGGGCGCCAGAGCCGCAAGCCCTCGCGGTTCCTCAACGGCATTGCGCCGCAGACGCGCGCCGA
TCCGGTGCCGGGCACATCCCGACGCAACCGGGGCGCCGCAGCGCGCTGCCGGATCTGTAACAACGAGCTGAACACTTCGG
CGGCCGTCATGCTGCGGCGGTGTGAGACGTGTGCAGCCGACGTCGACGAGGAGTTGTTGTTGCAGCTCAAGTCCTGGCGT
CTGAGCACCGCCAAGGAACAGAACGTCCCCGCCTATGTCGTTTTCACCGACAACACCCTGATCGCGATCGCCGAGCTGCT
ACCTACCGACGACGCGGCGCTGATCGCGATCCCCGGCATCGGTGCCCGCAAGCTGGAACAATACGGATCGGACGTGCTGC
AGCTGGTTCGCGGTCGGACCTGA

Upstream 100 bases:

>100_bases
CGACCCGGGCGGTGTGGCGGCTCGGCAGTTGTTCATGTCGCACGCCCCACGGCGTGGCGACACGCCCGGTCTGTCATCTA
CCGCTGCCAAGATGGACGTC

Downstream 100 bases:

>100_bases
ACCGGGCCTGTACCGGCAAACGCGCAGGTCAGAAAATCGGTTGTGGTCAGCTGCTGCCACCGGTTAACCTCCAGGTCGCA
TTCTGCTGCCAGCCTGGAGA

Product: ATP-dependent DNA helicase II uvrD2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MSIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVLAVTFTQRAAGEMRSRLRALD
AAARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQLLDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIG
PEEYVTAVAAARRDPPLDAAQIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDV
TPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRSTPQVVSLANRVIAAARGRVA
GSKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIASGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGF
FNRQEIKQALLALQRVSERDTDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAE
LRRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEERRLLYVGITRARVHLALSWA
LSRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAAARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWR
LSTAKEQNVPAYVVFTDNTLIAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT

Sequences:

>Translated_700_residues
MSIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVLAVTFTQRAAGEMRSRLRALD
AAARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQLLDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIG
PEEYVTAVAAARRDPPLDAAQIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDV
TPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRSTPQVVSLANRVIAAARGRVA
GSKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIASGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGF
FNRQEIKQALLALQRVSERDTDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAE
LRRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEERRLLYVGITRARVHLALSWA
LSRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAAARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWR
LSTAKEQNVPAYVVFTDNTLIAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT
>Mature_699_residues
SIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVLAVTFTQRAAGEMRSRLRALDA
AARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQLLDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIGP
EEYVTAVAAARRDPPLDAAQIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDVT
PLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRSTPQVVSLANRVIAAARGRVAG
SKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIASGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGFF
NRQEIKQALLALQRVSERDTDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAEL
RRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEERRLLYVGITRARVHLALSWAL
SRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAAARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWRL
STAKEQNVPAYVVFTDNTLIAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367296, Length=476, Percent_Identity=34.6638655462185, Blast_Score=219, Evalue=6e-58,
Organism=Escherichia coli, GI48994965, Length=659, Percent_Identity=30.1972685887709, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI48994977, Length=76, Percent_Identity=39.4736842105263, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=517, Percent_Identity=25.9187620889749, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324477, Length=388, Percent_Identity=21.9072164948454, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): UVRD_MYCBO (P64321)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856867.1
- ProteinModelPortal:   P64321
- EnsemblBacteria:   EBMYCT00000016706
- GeneID:   1094074
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3222c
- GeneTree:   EBGT00050000015808
- HOGENOM:   HBG467281
- OMA:   ITRRIAH
- ProtClustDB:   CLSK872151
- BioCyc:   MBOV233413:MB3222C-MONOMER
- GO:   GO:0005622
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997
- PANTHER:   PTHR11070
- SMART:   SM00341

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase; SSF47819 HRDC_like

EC number: =3.6.4.12

Molecular weight: Translated: 75604; Mature: 75473

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50967 HRDC; PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVL
CCCCCCCCEECCCCHHCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
AVTFTQRAAGEMRSRLRALDAAARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQL
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIGPEEYVTAVAAARRDPPLDAA
HHHHHHHHHHHHHHCEECCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
QIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDV
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCC
TPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRS
CHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCEEHHHHHHCCCHHEEEEHHHCCC
TPQVVSLANRVIAAARGRVAGSKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIA
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGFFNRQEIKQALLALQRVSERD
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
TDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LRRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHC
RRLLYVGITRARVHLALSWALSRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAA
CCEEEEEEEHHHEEEEEEEHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCH
ARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWRLSTAKEQNVPAYVVFTDNTL
HHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHH
IAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIASDPLIAGLDDQQREAVLAPRGPVCVLAGAGTGKTRTITHRIASLVASGHVAAGQVL
CCCCCCCEECCCCHHCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
AVTFTQRAAGEMRSRLRALDAAARTGSGVGAVQALTFHAAAYRQLRYFWSRVIADTGWQL
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LDSKFAVVARAASRTRLHASTDDVRDLAGEIEWAKASLIGPEEYVTAVAAARRDPPLDAA
HHHHHHHHHHHHHHCEECCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
QIAAVYSEYEALKARGDGVTLLDFDDLLLHTAAAIENDAAVAEEFQDRYRCFVVDEYQDV
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCC
TPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPRFLLDFSRRFPDAAVVRLERDYRS
CHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCEEHHHHHHCCCHHEEEEHHHCCC
TPQVVSLANRVIAAARGRVAGSKLRLSGQREPGPVPSFHEHSDEPAEAATVAASIARLIA
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SGTPPSEVAILYRVNAQSEVYEEALTQAGIAYQVRGGEGFFNRQEIKQALLALQRVSERD
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
TDAALSDVVRAVLAPLGLTAQPPVGTRARERWEALTALAELVDDELAQRPALQLPGLLAE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LRRRAEARHPPVVQGVTLASLHAAKGLEWDAVFLVGLADGTLPISHALAHGPNSEPVEEE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHC
RRLLYVGITRARVHLALSWALSRSPGGRQSRKPSRFLNGIAPQTRADPVPGTSRRNRGAA
CCEEEEEEEHHHEEEEEEEHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCH
ARCRICNNELNTSAAVMLRRCETCAADVDEELLLQLKSWRLSTAKEQNVPAYVVFTDNTL
HHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHH
IAIAELLPTDDAALIAIPGIGARKLEQYGSDVLQLVRGRT
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972