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The map label for this gene is moaE1

Identifier: 148824312

GI number: 148824312

Start: 3498255

End: 3498698

Strand: Direct

Name: moaE1

Synonym: TBFG_13139

Alternate gene names: 148824312

Gene position: 3498255-3498698 (Clockwise)

Preceding gene: 148824311

Following gene: 148824313

Centisome position: 79.07

GC content: 57.88

Gene sequence:

>444_bases
ATGGCCAATGTGGTAGCTGAAGGTGCCTACCCTTACTGTCGGCTCACTGATCAGCCGCTGAGTGTGGACGAAGTGCTAGC
CGCCGTCTCGGGCCCCGAACAAGGCGGCATTGTCATATTTGTGGGAAACGTGCGTGACCACAATGCCGGGCATGATGTCA
CGCGGTTGTTCTACGAGGCGTATCCGCCGATGGTGATTCGGACATTGATGTCGATCATCGGACGGTGTGAAGACAAGGCC
GAGGGTGTCCGCGTTGCTGTCGCGCACCGGACCGGTGAATTGCAAATCGGTGATGCCGCGGTCGTTATTGGCGCGTCAGC
TCCCCACCGTGCGGAGGCATTTGACGCCGCGCGTATGTGTATCGAGTTGCTTAAGCAGGAAGTGCCGATTTGGAAGAAGG
AATTCAGCTCGACCGGTGCTGAATGGGTCGGCGATAGACCATGA

Upstream 100 bases:

>100_bases
TGTCGGCGGCCGGCAACGGCGACGCGGTGGTGCAGCCCTCGGGCGCGGGCATCCACGAGGTAGACGTCAAGATCACCTGA
TAGCTAGGAAGGATGTCTGA

Downstream 100 bases:

>100_bases
GTCCGTCTCCATCGGCCCTGCTCGCCGACCACCCGGACCGCATTCGTTGGAACGCGAAATACGAGTGCGCTGACCCCACG
GAGGCGGTATTTGCGCCCAT

Product: molybdenum cofactor biosynthesis protein E moaE1

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E 1; Molybdopterin-converting factor large subunit 1; Molybdopterin-converting factor subunit 2

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MANVVAEGAYPYCRLTDQPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEAYPPMVIRTLMSIIGRCEDKA
EGVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMCIELLKQEVPIWKKEFSSTGAEWVGDRP

Sequences:

>Translated_147_residues
MANVVAEGAYPYCRLTDQPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEAYPPMVIRTLMSIIGRCEDKA
EGVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMCIELLKQEVPIWKKEFSSTGAEWVGDRP
>Mature_146_residues
ANVVAEGAYPYCRLTDQPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEAYPPMVIRTLMSIIGRCEDKAE
GVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMCIELLKQEVPIWKKEFSSTGAEWVGDRP

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family

Homologues:

Organism=Homo sapiens, GI4758732, Length=142, Percent_Identity=36.6197183098592, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1787003, Length=130, Percent_Identity=38.4615384615385, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17509279, Length=129, Percent_Identity=37.2093023255814, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356259, Length=138, Percent_Identity=31.8840579710145, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAE1_MYCTU (O05795)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   F70921
- RefSeq:   NP_337727.1
- RefSeq:   YP_177931.1
- PDB:   2WP4
- PDBsum:   2WP4
- ProteinModelPortal:   O05795
- SMR:   O05795
- EnsemblBacteria:   EBMYCT00000000652
- EnsemblBacteria:   EBMYCT00000071991
- GeneID:   888811
- GeneID:   923487
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3201
- KEGG:   mtu:Rv3119
- TIGR:   MT3201
- TubercuList:   Rv3119
- GeneTree:   EBGT00050000017227
- HOGENOM:   HBG460564
- OMA:   ENTPYPE
- ProtClustDB:   CLSK799956
- InterPro:   IPR003448
- Gene3D:   G3DSA:3.90.1170.40
- PANTHER:   PTHR23404:SF2

Pfam domain/function: PF02391 MoaE; SSF54690 Mb_biosynth_MoaE

EC number: NA

Molecular weight: Translated: 15920; Mature: 15789

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVAEGAYPYCRLTDQPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEA
CCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
YPPMVIRTLMSIIGRCEDKAEGVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMC
CCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCEEEEEECCCCCHHHHHHHHHHH
IELLKQEVPIWKKEFSSTGAEWVGDRP
HHHHHHHCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ANVVAEGAYPYCRLTDQPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEA
CCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
YPPMVIRTLMSIIGRCEDKAEGVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMC
CCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCEEEEEECCCCCHHHHHHHHHHH
IELLKQEVPIWKKEFSSTGAEWVGDRP
HHHHHHHCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036