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The map label for this gene is 148824279

Identifier: 148824279

GI number: 148824279

Start: 3464686

End: 3466107

Strand: Direct

Name: 148824279

Synonym: TBFG_13104

Alternate gene names: NA

Gene position: 3464686-3466107 (Clockwise)

Preceding gene: 148824278

Following gene: 148824280

Centisome position: 78.31

GC content: 69.97

Gene sequence:

>1422_bases
ATGATGCGCAGGCTCAACGGCGTTGACGCGCTGATGCTGTATCTCGACGGCGGCAGCGCCTACAACCACACCCTCAAGAT
CAGCGTGCTCGACCCGTCGACCGACCCGGACGGCTGGTCGTGGCCGAAGGCGCGGCAGATGTTCGAGGAGCGCGCCCACC
TGCTTCCGGTCTTCCGGCTGCGGTACCTGCCCACACCGCTGGGCCTGCATCACCCGATCTGGGTCGAGGATCCCGAATTC
GACCTCGACGCGCACGTGCGCCGGGTCGTCTGTCCCGCCCCGGGCGGGATGGCGGAATTCTGCGCGCTCGTCGAGCAGAT
CTACGCCCACCCGCTGGATCGCGACCGCCCGCTGTGGCAGACCTGGGTGGTCGAGGGCCTCGACGGCGGCCGCGTCGCCC
TGGTCACGCTGCTGCACCACGCCTACTCCGACGGCGTCGGCGTGCTGGACATGCTCGCCGCGTTCTACAACGACACGCCT
GACGAGGCCCCCGTGGTTGCGCCCCCGTGGGAGCCGCCGCCGCTGCCGTCCACCCGGCAACGCCTCGGTTGGGCCCTGCG
GGACCTGCCCTCCAGGCTCGGCAAGATCGCGCCGACCGTGCGGGCCGTTCGTGATCGGGTGCGCATCGAACGGGAGTTCG
CCAAAGACGGCGACCGGCGCGTCCCGCCCACGTTCGACCGCTCCGCACCGCCGGGCCCGTTTCAGCGCGGGCTGTCGCGC
AGCCGGCGGTTCTCCTGCGAATCGTTCCCGCTCGCCGAGGTTCGCGAGGTGAGCAAGACGCTGGGCGTCACCATCAACGA
CGTCTTTTTGGCGTGTGTGGCCGGTGCCGTTCGTCGCTATCTGGAGCGTTGCGGCTCCCCTCCCACCGACGCGATGGTGG
CCACGATGCCGCTCGCGGTCACCCCGGCGGCCGAGCGCGCCCACCCCGGCAACTACTCGTCGGTCGACTACGTCTGGCTA
CGCGCCGACATCGCCGACCCGCTCGAGCGGCTACACGCGACCCACCTCGCCGCCGAGGCCACCAAGCAGCACTTCGCCCA
GACCAAGGACGCCGACGTCGGCGCGGTGGTCGAGCTGCTGCCGGAACGCCTCATCTCGGGCCTGGCGCGTGCCAACGCGC
GCACCAAGGGCCGCTTCGACACCTTCAAGAACGTGGTCGTGTCCAACGTGCCGGGGCCGCGTGAGCCGCGGTATCTCGGC
CGCTGGCGCGTCGACCAGTGGTTTTCCACCGGGCAGATCTCCCACGGCGCCACGCTCAACATGACCGTCTGGAGCTATTG
CGACCAGTTCAACCTGTGCGTAATGGCCGACGCAGTCGCGGTTCGGAACACCTGGGAATTGCTCGGCGGCTTCCGCGCCT
CGCACGAGGAGCTGCTCGCGGCGGCCCGTGCCCAAGCCACGCCCAAGGAGATGGCCACATGA

Upstream 100 bases:

>100_bases
CAGGTGAACCAGGGCTACCAGGATCTGCGGGACGGCAAGAACATTCGGGGCGTGATCGTGCACTGACCAGCTTCCACCAA
CCACGAATCCAGAGAGGACG

Downstream 100 bases:

>100_bases
CCCGCATCAATCCGATCGATCTGTCCTTCCTGCTGCTGGAGCGGGCCAACCGGCCCAACCACATGGCCGCCTACACGATC
TTCGAAAAGCCGAAAGGACA

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3087/MT3172 [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MMRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEF
DLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTP
DEAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSR
SRRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWL
RADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLG
RWRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT

Sequences:

>Translated_473_residues
MMRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEF
DLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTP
DEAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSR
SRRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWL
RADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLG
RWRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
>Mature_473_residues
MMRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEF
DLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTP
DEAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSR
SRRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWL
RADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLG
RWRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 52729; Mature: 52729

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRL
CCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RYLPTPLGLHHPIWVEDPEFDLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQ
HHCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
TWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPDEAPVVAPPWEPPPLPSTRQ
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHH
RLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
SRRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAV
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEE
TPAAERAHPGNYSSVDYVWLRADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELL
CCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
PERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGRWRVDQWFSTGQISHGATLN
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCEEE
MTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
EHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MMRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRL
CCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RYLPTPLGLHHPIWVEDPEFDLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQ
HHCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
TWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPDEAPVVAPPWEPPPLPSTRQ
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHH
RLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
SRRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAV
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEE
TPAAERAHPGNYSSVDYVWLRADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELL
CCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
PERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGRWRVDQWFSTGQISHGATLN
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCEEE
MTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
EHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]