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The map label for this gene is recG

Identifier: 148824163

GI number: 148824163

Start: 3339206

End: 3341419

Strand: Reverse

Name: recG

Synonym: TBFG_12988

Alternate gene names: 148824163

Gene position: 3341419-3339206 (Counterclockwise)

Preceding gene: 148824164

Following gene: 148824162

Centisome position: 75.52

GC content: 64.59

Gene sequence:

>2214_bases
GTGGCGTCGTTAAGCGATCGGCTCGATCGCGTCCTCGGCGCCACGGCAGCCGATGCCCTCGACGAGCAGTTCGGCATGCG
GACCGTCGACGACCTGCTCCGCCACTACCCGCGCAGCTACGTCGAGGGCGCGGCGCGGGTGGGTATCGGTGACGCGCGAC
CGGAAGCTGGCGAGCACATCACCATCGTCGACGTGATCACCGATACGTATTCGTTTCCGATGAAAAAGAAGCCGAATCGG
AAATGTCTGCGCATCACCGTCGGCGGCGGCCGCAACAAGGTGACGGCCACGTTCTTCAACGCGGACTACATCATGCGGGA
CCTCACCAAGCACACGAAGGTGATGCTCTCCGGGGAAGTCGGCTACTACAAGGGCGCGATGCAGCTCACGCATCCGGCGT
TTCTCATCCTCGACTCGCCGGACGGGAAGAACCACGGCACCAGGTCGCTCAAAAGTATCGCTGATGCCTCCAAGGCCATC
AGCGGTGAATTGGTCGTGGAGGAGTTCGAGCGTCGTTTCTTCCCGATCTATCCGGCCAGCACAAAAGTGCAGAGCTGGGA
CATCTTCAAATGCGTGCGGCAGGTGCTCGATGTTCTCGACCGGGTCGATGATCCGTTGCCCGCGGAACTACGCGCCAAGC
ACGGCCTGATCCCCGAGGACGAGGCACTGCGCGCCATTCACCTTGCCGAAAGCCAGTCGTTACGCGAGCGCGCCCGGGAA
CGCCTGACCTTCGACGAGGCCGTGGGTCTGCAGTGGGCGCTGGTGGCCCGACGGCACGGTGAACTGTCGGAATCCGGGCC
CTCGGCGGCCTGGAAATCTAACGGTCTTGCCGCTGAGCTACTGCGGCGGTTGCCTTTTGAGCTGACGGCGGGACAGCGTG
AGGTGCTCGACGTGTTGTCCGACGGGCTCGCGGCGAACCGCCCGCTGAATCGCCTGCTGCAAGGCGAAGTGGGCTCGGGC
AAAACGATCGTTGCGGTGTTGGCGATGCTGCAGATGGTGGACGCGGGTTACCAGTGTGCCCTGCTGGCGCCAACGGAAGT
CCTTGCCGCACAACACTTGCGATCGATCCGCGATGTCCTAGGGCCGCTGGCCATGGGCGGCCAACTGGGAGGCGCCGAAA
ACGCCACCCGGGTGGCACTGCTCACCGGTTCGATGACGGCAGGGCAGAAGAAGCAAGTTCGCGCCGAAATCGCCAGCGGT
CAGGTCGGCATCGTCATCGGCACGCACGCGCTGCTGCAGGAGGCCGTCGACTTTCACAACCTGGGCATGGTGGTGGTCGA
CGAGCAACACCGGTTTGGTGTCGAGCAGCGAGATCAGTTGCGCGCCAAGGCCCCCGCCGGCATTACCCCGCACCTGCTGG
TGATGACCGCGACGCCGATACCGCGCACGGTCGCGCTGACCGTCTATGGCGACCTGGAAACCTCTACGCTGCGCGAACTT
CCGCTCGGGCGCCAGCCGATTGCCACCAACGTCATCTTTGTCAAGGACAAGCCCGCCTGGCTCGACCGCGCCTGGCGGCG
CATCATCGAAGAGGCTGCCGCCGGCCGCCAGGCCTATGTGGTGGCGCCCCGCATCGATGAGTCCGACGACACCGACGTTC
AAGGCGGCGTCCGACCATCGGCCACTGCCGAAGGCCTGTTCTCCCGGTTGCGTTCCGCCGAGCTCGCAGAGTTGCGGTTG
GCGCTCATGCATGGACGGTTGTCGGCCGACGACAAGGACGCTGCGATGGCGGCTTTCCGGGCCGGTGAGGTCGATGTGCT
GGTGTGCACCACGGTCATTGAGGTTGGCGTCGATGTCCCCAACGCTACGGTCATGCTGGTGATGGACGCCGACCGGTTCG
GCATCAGCCAATTGCACCAGCTGCGCGGCCGCATCGGGCGCGGCGAACATCCCAGCGTGTGTCTGCTGGCCAGCTGGGTG
CCGCCGGATACGCCGGCGGGTCAGCGGCTGCGTGCGGTCGCCGGGACAATGGACGGGTTCGCCCTCGCCGATCTGGATCT
GAAGGAGCGCAAGGAGGGAGATGTGCTGGGCCGCAACCAATCCGGCAAGGCCATCACGCTGCGGTTGCTGTCATTGGCCG
AACACGAGGAATACATAGTGGCCGCGCGGGACTTCTGCATCGAGGCTTACAAGAACCCCACAGACCCCGCATTGGCGCTG
ATGGCCGCGCGATTCACCAGCACCGACCGCATCGAATACTTAGATAAGTCATGA

Upstream 100 bases:

>100_bases
TCCTGGAACGGCATGTGCACGACCACCATCCAGGCACCGAGCTGGTCTCCTACCGCACCGGACACCGCGGCGACGCGCTG
CTGATCGGGGTCGAGTAGCG

Downstream 100 bases:

>100_bases
ACCGCAGAACGCTACTGTGGCTGTCGGCGATCGCGGCGCTGGCGCTGGTGGTCGCGTATCAGACGCTGGGATCATCGGCG
GGCAGGCACGCTGACGAGTT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 736

Protein sequence:

>737_residues
MASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHITIVDVITDTYSFPMKKKPNR
KCLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEVGYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAI
SGELVVEEFERRFFPIYPASTKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARE
RLTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLSDGLAANRPLNRLLQGEVGSG
KTIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVLGPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASG
QVGIVIGTHALLQEAVDFHNLGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLREL
PLGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPSATAEGLFSRLRSAELAELRL
ALMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVPNATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWV
PPDTPAGQRLRAVAGTMDGFALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALAL
MAARFTSTDRIEYLDKS

Sequences:

>Translated_737_residues
MASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHITIVDVITDTYSFPMKKKPNR
KCLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEVGYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAI
SGELVVEEFERRFFPIYPASTKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARE
RLTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLSDGLAANRPLNRLLQGEVGSG
KTIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVLGPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASG
QVGIVIGTHALLQEAVDFHNLGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLREL
PLGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPSATAEGLFSRLRSAELAELRL
ALMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVPNATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWV
PPDTPAGQRLRAVAGTMDGFALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALAL
MAARFTSTDRIEYLDKS
>Mature_736_residues
ASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHITIVDVITDTYSFPMKKKPNRK
CLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEVGYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAIS
GELVVEEFERRFFPIYPASTKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARER
LTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLSDGLAANRPLNRLLQGEVGSGK
TIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVLGPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASGQ
VGIVIGTHALLQEAVDFHNLGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLRELP
LGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPSATAEGLFSRLRSAELAELRLA
LMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVPNATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWVP
PDTPAGQRLRAVAGTMDGFALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALALM
AARFTSTDRIEYLDKS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367254, Length=686, Percent_Identity=36.0058309037901, Blast_Score=356, Evalue=3e-99,
Organism=Escherichia coli, GI1787357, Length=411, Percent_Identity=35.5231143552311, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECG_MYCBO (P64323)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856643.1
- ProteinModelPortal:   P64323
- SMR:   P64323
- EnsemblBacteria:   EBMYCT00000016194
- GeneID:   1093419
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2998c
- GeneTree:   EBGT00050000015872
- HOGENOM:   HBG747311
- OMA:   NKGLQPH
- ProtClustDB:   PRK10917
- BioCyc:   MBOV233413:MB2998C-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00643

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; SSF50249 Nucleic_acid_OB

EC number: =3.6.4.12

Molecular weight: Translated: 80362; Mature: 80231

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHI
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCCCEE
TIVDVITDTYSFPMKKKPNRKCLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEV
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHEEEEECCC
GYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAISGELVVEEFERRFFPIYPAS
CCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCC
TKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARE
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLS
HCCHHHHHCCHHEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHH
DGLAANRPLNRLLQGEVGSGKTIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVL
CCHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHH
GPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASGQVGIVIGTHALLQEAVDFHN
HHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
LGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLREL
CCEEEEECHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEEECCCHHHHHHC
PLGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPS
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
ATAEGLFSRLRSAELAELRLALMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCCCC
NATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWVPPDTPAGQRLRAVAGTMDGF
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCC
ALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALAL
EEECCCHHHCCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
MAARFTSTDRIEYLDKS
HHHHCCCCCHHHHCCCC
>Mature Secondary Structure 
ASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHI
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCCCEE
TIVDVITDTYSFPMKKKPNRKCLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEV
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHEEEEECCC
GYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAISGELVVEEFERRFFPIYPAS
CCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCC
TKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARE
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLS
HCCHHHHHCCHHEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHH
DGLAANRPLNRLLQGEVGSGKTIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVL
CCHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHH
GPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASGQVGIVIGTHALLQEAVDFHN
HHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
LGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLREL
CCEEEEECHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEEECCCHHHHHHC
PLGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPS
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
ATAEGLFSRLRSAELAELRLALMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCCCC
NATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWVPPDTPAGQRLRAVAGTMDGF
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCC
ALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALAL
EEECCCHHHCCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
MAARFTSTDRIEYLDKS
HHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972