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The map label for this gene is orfB [H]

Identifier: 148824136

GI number: 148824136

Start: 3307823

End: 3309313

Strand: Reverse

Name: orfB [H]

Synonym: TBFG_12961

Alternate gene names: 148824136

Gene position: 3309313-3307823 (Counterclockwise)

Preceding gene: 148824137

Following gene: 148824135

Centisome position: 74.8

GC content: 65.86

Gene sequence:

>1491_bases
GTGATAGAGGAGCAACGGACGATGAGCGTTGAAGGTGCCGACCAGCAGAGCGAAAAGCTTTTTCACTACCTAAAGAAGGT
TGCTGTCGAGCTCGATGAGACACGCGCTCGGCTGCGTGAATATGAGCAACGCGCGACAGAACCGGTGGCGGTGGTGGGAA
TCGGCTGCCGATTTCCGGGCGGCGTGGATGGCCCGGACGGACTCTGGGATGTCGTTTCGGCGGGGCGCGACGTGGTGTCG
GAGTTTCCGACCGATCGGGGCTGGGATGTTGAGGGGCTATACGATCCGGATCCCGACGCTGAGGGCAAAACCTACACGCG
ATGGGGGGCATTTCTAGACGACGCCACCGGCTTTGACGCCGGGTTCTTCGGCATCGCTCCTAGTGAAGTGTTGGCGATGG
ATCCTCAGCAGCGGTTGATGCTGGAGGTTTCGTGGGAGGCGTTGGAGCACGCGGGGATTGACCCGTTGTCGTTGCGTGGT
TCGGCGACCGGGGTGTACACCGGCATTTTCGCGGCGAGTTACGGCAATAGGGATACCGGGGGGCTGCAAGGGTACGGGTT
GACCGGCACGTCAATCAGCGTGGCCTCGGGACGTGTGTCGTACGTATTGGGGCTGCAGGGCCCGGCGGTGTCAGTGGATA
CCGCTTGTTCGTCGTCGTTGGTGGCGATCCATTGGGCGATGTCGTCATTGCGCTCCGGGGAGTGTGATTTGGCGTTGGCC
GGTGGGGTGACGGTGATGGGGCTGCCGTCGATTTTTGTGGGGTTTTCCCGGCAGCGGGGGTTGGCTGCCGATGGGCGATG
TAAGGCGTTTGCGGCCGCGGCCGACGGCACCGGGTGGGGCGAGGGCGCCGGGGTGGTTGTGCTGGAGCGGCTATCGGACG
CCCGGCGGTTGGGGCATTCGGTGCTGGCGGTGGTGCGGGGGAGCGCGGTCAACCAGGATGGTGCGTCCAATGGGCTGACC
GCGCCCAATGGGCTCGCCCAGCAGCGAGTGATCCAGGCGGCGTTGGCCAACGCCGGGTTGTCCGCCGCAGACGTAGACGT
GGTGGAGGCACACGGGACCGCCACCACGTTGGGTGATCCCATCGAAGCGCAGGCATTGCTGTCCACCTACGGGCAGGGAG
GTCCAGCGGAGCAGCCGCTGTGGGTGGGCTCAATCAAGTCGAACATGGGCCACACCCAGGCCGCCGCGGGGGTCGCCGGG
GTGATCAAGATGGTGCAGGCGATGCGTCATGGGGTGATGCCGGCGACGTTGCATGTGGATGAGCCTTCCCCGCGGGTGGA
TTGGACCAGCGGCGCGGTGTCGGTGTTGACCGAGGCCAGGGAGTGGTCGGTTGACGGGCGTCCGCGCCGGGCGGCGGTGT
CCTCCTTTGGGATCAGCGGCACCAACGCGCATCTGATCTTGGAAGAGGCCCCCGTACCAGCCCCCGCAGAGGCGCCGGTT
GAGGCGAGCGAAAGCACCGGGGGCCGCGGCCGTCGATGGTGCCGTGGGTGA

Upstream 100 bases:

>100_bases
TGGGCACCATCGCCGGCAGCGAGGCGGGCCTGGGCAAGCTGATCCAGGCTGCTTCGACTCCTGACGAAATTTTCCAGCTA
ATCGATTCCGAACTTGGGAA

Downstream 100 bases:

>100_bases
TTTCGGCGAGATCGGCTGAGGCGTTGACGGCGCAGGCGGGTCGACTTATGGCCCACGTGCAGGCCAACCCAGGGCTGGAT
CCGATCGATGTGGGGTGCTC

Product: polyketide synthase pks15

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MIEEQRTMSVEGADQQSEKLFHYLKKVAVELDETRARLREYEQRATEPVAVVGIGCRFPGGVDGPDGLWDVVSAGRDVVS
EFPTDRGWDVEGLYDPDPDAEGKTYTRWGAFLDDATGFDAGFFGIAPSEVLAMDPQQRLMLEVSWEALEHAGIDPLSLRG
SATGVYTGIFAASYGNRDTGGLQGYGLTGTSISVASGRVSYVLGLQGPAVSVDTACSSSLVAIHWAMSSLRSGECDLALA
GGVTVMGLPSIFVGFSRQRGLAADGRCKAFAAAADGTGWGEGAGVVVLERLSDARRLGHSVLAVVRGSAVNQDGASNGLT
APNGLAQQRVIQAALANAGLSAADVDVVEAHGTATTLGDPIEAQALLSTYGQGGPAEQPLWVGSIKSNMGHTQAAAGVAG
VIKMVQAMRHGVMPATLHVDEPSPRVDWTSGAVSVLTEAREWSVDGRPRRAAVSSFGISGTNAHLILEEAPVPAPAEAPV
EASESTGGRGRRWCRG

Sequences:

>Translated_496_residues
MIEEQRTMSVEGADQQSEKLFHYLKKVAVELDETRARLREYEQRATEPVAVVGIGCRFPGGVDGPDGLWDVVSAGRDVVS
EFPTDRGWDVEGLYDPDPDAEGKTYTRWGAFLDDATGFDAGFFGIAPSEVLAMDPQQRLMLEVSWEALEHAGIDPLSLRG
SATGVYTGIFAASYGNRDTGGLQGYGLTGTSISVASGRVSYVLGLQGPAVSVDTACSSSLVAIHWAMSSLRSGECDLALA
GGVTVMGLPSIFVGFSRQRGLAADGRCKAFAAAADGTGWGEGAGVVVLERLSDARRLGHSVLAVVRGSAVNQDGASNGLT
APNGLAQQRVIQAALANAGLSAADVDVVEAHGTATTLGDPIEAQALLSTYGQGGPAEQPLWVGSIKSNMGHTQAAAGVAG
VIKMVQAMRHGVMPATLHVDEPSPRVDWTSGAVSVLTEAREWSVDGRPRRAAVSSFGISGTNAHLILEEAPVPAPAEAPV
EASESTGGRGRRWCRG
>Mature_496_residues
MIEEQRTMSVEGADQQSEKLFHYLKKVAVELDETRARLREYEQRATEPVAVVGIGCRFPGGVDGPDGLWDVVSAGRDVVS
EFPTDRGWDVEGLYDPDPDAEGKTYTRWGAFLDDATGFDAGFFGIAPSEVLAMDPQQRLMLEVSWEALEHAGIDPLSLRG
SATGVYTGIFAASYGNRDTGGLQGYGLTGTSISVASGRVSYVLGLQGPAVSVDTACSSSLVAIHWAMSSLRSGECDLALA
GGVTVMGLPSIFVGFSRQRGLAADGRCKAFAAAADGTGWGEGAGVVVLERLSDARRLGHSVLAVVRGSAVNQDGASNGLT
APNGLAQQRVIQAALANAGLSAADVDVVEAHGTATTLGDPIEAQALLSTYGQGGPAEQPLWVGSIKSNMGHTQAAAGVAG
VIKMVQAMRHGVMPATLHVDEPSPRVDWTSGAVSVLTEAREWSVDGRPRRAAVSSFGISGTNAHLILEEAPVPAPAEAPV
EASESTGGRGRRWCRG

Specific function: May be involved in the biosynthesis of the oleandomycin lactone ring [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=429, Percent_Identity=35.6643356643357, Blast_Score=248, Evalue=9e-66,
Organism=Homo sapiens, GI8923559, Length=280, Percent_Identity=31.4285714285714, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI310129082, Length=100, Percent_Identity=38, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310109937, Length=100, Percent_Identity=38, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI239744023, Length=100, Percent_Identity=38, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1787337, Length=287, Percent_Identity=31.3588850174216, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788663, Length=304, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI212642053, Length=441, Percent_Identity=36.5079365079365, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17550940, Length=428, Percent_Identity=32.4766355140187, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI71983541, Length=282, Percent_Identity=32.6241134751773, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71983535, Length=270, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320904, Length=352, Percent_Identity=28.4090909090909, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI221330659, Length=444, Percent_Identity=36.7117117117117, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI19920632, Length=444, Percent_Identity=36.7117117117117, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24581345, Length=457, Percent_Identity=34.3544857768052, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24644341, Length=468, Percent_Identity=29.7008547008547, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR000794
- InterPro:   IPR002198
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020801
- InterPro:   IPR020841
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR020802
- InterPro:   IPR015083
- InterPro:   IPR006162
- InterPro:   IPR001031
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF00106 adh_short; PF08990 Docking; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.3.1.41

Molecular weight: Translated: 51674; Mature: 51674

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEQRTMSVEGADQQSEKLFHYLKKVAVELDETRARLREYEQRATEPVAVVGIGCRFPG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GVDGPDGLWDVVSAGRDVVSEFPTDRGWDVEGLYDPDPDAEGKTYTRWGAFLDDATGFDA
CCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCC
GFFGIAPSEVLAMDPQQRLMLEVSWEALEHAGIDPLSLRGSATGVYTGIFAASYGNRDTG
CCCCCCCHHHEECCCHHHEEEEEEHHHHHHCCCCCEEECCCCCHHHHHHEEECCCCCCCC
GLQGYGLTGTSISVASGRVSYVLGLQGPAVSVDTACSSSLVAIHWAMSSLRSGECDLALA
CCCCCCCCCCEEEECCCCEEEEEECCCCCEEECHHCCCCHHHHHHHHHHHCCCCCCEEEE
GGVTVMGLPSIFVGFSRQRGLAADGRCKAFAAAADGTGWGEGAGVVVLERLSDARRLGHS
CCEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHH
VLAVVRGSAVNQDGASNGLTAPNGLAQQRVIQAALANAGLSAADVDVVEAHGTATTLGDP
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
IEAQALLSTYGQGGPAEQPLWVGSIKSNMGHTQAAAGVAGVIKMVQAMRHGVMPATLHVD
HHHHHHHHHCCCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
EPSPRVDWTSGAVSVLTEAREWSVDGRPRRAAVSSFGISGTNAHLILEEAPVPAPAEAPV
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
EASESTGGRGRRWCRG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEEQRTMSVEGADQQSEKLFHYLKKVAVELDETRARLREYEQRATEPVAVVGIGCRFPG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GVDGPDGLWDVVSAGRDVVSEFPTDRGWDVEGLYDPDPDAEGKTYTRWGAFLDDATGFDA
CCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCC
GFFGIAPSEVLAMDPQQRLMLEVSWEALEHAGIDPLSLRGSATGVYTGIFAASYGNRDTG
CCCCCCCHHHEECCCHHHEEEEEEHHHHHHCCCCCEEECCCCCHHHHHHEEECCCCCCCC
GLQGYGLTGTSISVASGRVSYVLGLQGPAVSVDTACSSSLVAIHWAMSSLRSGECDLALA
CCCCCCCCCCEEEECCCCEEEEEECCCCCEEECHHCCCCHHHHHHHHHHHCCCCCCEEEE
GGVTVMGLPSIFVGFSRQRGLAADGRCKAFAAAADGTGWGEGAGVVVLERLSDARRLGHS
CCEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHH
VLAVVRGSAVNQDGASNGLTAPNGLAQQRVIQAALANAGLSAADVDVVEAHGTATTLGDP
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
IEAQALLSTYGQGGPAEQPLWVGSIKSNMGHTQAAAGVAGVIKMVQAMRHGVMPATLHVD
HHHHHHHHHCCCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
EPSPRVDWTSGAVSVLTEAREWSVDGRPRRAAVSSFGISGTNAHLILEEAPVPAPAEAPV
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
EASESTGGRGRRWCRG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8107683 [H]