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The map label for this gene is ispG [H]

Identifier: 148824059

GI number: 148824059

Start: 3190898

End: 3192061

Strand: Reverse

Name: ispG [H]

Synonym: TBFG_12884

Alternate gene names: 148824059

Gene position: 3192061-3190898 (Counterclockwise)

Preceding gene: 148824060

Following gene: 148824058

Centisome position: 72.15

GC content: 65.21

Gene sequence:

>1164_bases
GTGACTGTAGGCTTGGGCATGCCGCAGCCCCCGGCACCCACGCTCGCTCCCCGGCGCGCCACCCGTCAGCTGATGGTCGG
CAACGTCGGCGTGGGCAGTGACCATCCGGTCTCGGTGCAATCGATGTGCACCACCAAAACCCACGACGTCAACTCGACAT
TGCAACAAATCGCCGAGCTGACCGCGGCCGGATGCGACATCGTGCGGGTGGCCTGCCCGCGCCAGGAGGACGCCGACGCG
CTGGCCGAGATCGCCCGGCACAGCCAGATCCCGGTAGTCGCGGACATACATTTCCAGCCGCGCTACATATTCGCCGCCAT
CGACGCTGGATGTGCCGCGGTGCGGGTCAACCCGGGCAACATCAAGGAGTTTGACGGCCGGGTGGGTGAGGTCGCCAAGG
CGGCGGGTGCGGCCGGGATCCCGATCCGAATCGGTGTCAACGCCGGTTCGCTGGACAAACGGTTCATGGAGAAGTATGGC
AAAGCCACGCCCGAGGCGCTGGTTGAGTCGGCGCTGTGGGAGGCTTCGCTTTTCGAGGAGCATGGCTTCGGTGACATCAA
GATCAGCGTCAAGCACAACGACCCGGTGGTGATGGTCGCCGCCTACGAGCTGCTTGCTGCACGGTGCGACTACCCACTGC
ACCTCGGTGTCACCGAGGCCGGCCCTGCTTTCCAGGGCACCATCAAGTCCGCGGTTGCCTTCGGCGCGTTGCTGTCGCGG
GGCATAGGCGACACCATCCGGGTGTCGTTGTCGGCCCCGCCGGTCGAGGAAGTCAAGGTGGGCAATCAGGTTCTCGAGTC
GTTGAACCTGCGGCCGCGTTCGCTCGAGATCGTGTCTTGCCCGTCGTGCGGTCGCGCGCAAGTCGACGTCTACACCCTGG
CCAACGAGGTAACCGCCGGCCTGGATGGTCTCGATGTGCCGTTGCGGGTGGCCGTGATGGGGTGTGTCGTCAATGGTCCG
GGTGAAGCACGTGAGGCCGACCTGGGCGTGGCGTCCGGCAACGGCAAAGGTCAGATCTTTGTACGGGGCGAAGTGATCAA
GACCGTGCCCGAAGCACAGATCGTCGAGACGCTGATCGAGGAGGCGATGCGGCTGGCCGCCGAAATGGGCGAGCAAGATC
CGGGCGCGACACCGAGCGGTTCGCCTATTGTGACCGTAAGCTGA

Upstream 100 bases:

>100_bases
TATGTGGTCTTGGTTCTTGTCGTCGGGTACATGCTCTTGACCGTCACCGCCGACCTGGTCAACCCGATTAGGCTTTTCCA
GTAGGTAGAAAGGATTTTCA

Downstream 100 bases:

>100_bases
TAGGGGTCAGCTTTTCATTCAGTGGCCGTCAGTTCCCACCAAAGGGAGTTCGCAGATGTCGGCTCCGCCCATCTCTCGGC
TTGTCGGCGAGCGGCAAGTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADA
LAEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYG
KATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
GIGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPGATPSGSPIVTVS

Sequences:

>Translated_387_residues
MTVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADA
LAEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYG
KATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
GIGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPGATPSGSPIVTVS
>Mature_386_residues
TVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADAL
AEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYGK
ATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRG
IGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCVVNGPG
EAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPGATPSGSPIVTVS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=326, Percent_Identity=46.319018404908, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40483; Mature: 40352

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAEL
CEECCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
TAAGCDIVRVACPRQEDADALAEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGN
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCC
IKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEE
CHHHCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
CCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAG
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHCC
LDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIE
CCCCCCHHHEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHH
EAMRLAAEMGEQDPGATPSGSPIVTVS
HHHHHHHHCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
TVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAEL
EECCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
TAAGCDIVRVACPRQEDADALAEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGN
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCC
IKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEE
CHHHCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSR
CCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAG
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHCC
LDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIE
CCCCCCHHHEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHH
EAMRLAAEMGEQDPGATPSGSPIVTVS
HHHHHHHHCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA