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The map label for this gene is ald

Identifier: 148823968

GI number: 148823968

Start: 3098775

End: 3099890

Strand: Direct

Name: ald

Synonym: TBFG_12793

Alternate gene names: 148823968

Gene position: 3098775-3099890 (Clockwise)

Preceding gene: 148823963

Following gene: 148823975

Centisome position: 70.04

GC content: 64.16

Gene sequence:

>1116_bases
ATGCGCGTCGGTATTCCGACCGAGACCAAAAACAACGAATTCCGGGTGGCCATCACCCCGGCCGGCGTCGCGGAACTAAC
CCGTCGTGGCCATGAGGTGCTCATCCAGGCAGGTGCCGGAGAGGGCTCGGCTATCACCGACGCGGATTTCAAGGCGGCAG
GCGCGCAACTGGTCGGCACCGCCGACCAGGTGTGGGCCGACGCTGATTTATTGCTCAAGGTCAAAGAACCGATAGCGGCG
GAATACGGCCGCCTGCGACACGGGCAGATCTTGTTCACGTTCTTGCATTTGGCCGCGTCACGTGCTTGCACCGATGCGTT
GTTGGATTCCGGCACCACGTCAATTGCCTACGAGACCGTCCAGACCGCCGACGGCGCACTACCCCTGCTTGCCCCGATGA
GCGAAGTCGCCGGTCGACTCGCCGCCCAGGTTGGCGCTTACCACCTGATGCGAACCCAAGGGGGCCGCGGTGTGCTGATG
GGCGGGGTGCCCGGCGTCGAACCGGCCGACGTCGTGGTGATCGGCGCCGGCACCGCCGGCTACAACGCAGCCCGCATCGC
CAACGGCATGGGCGCGACCGTTACGGTTCTAGACATCAACATCGACAAACTTCGGCAACTCGACGCCGAGTTCTGCGGCC
GGATCCACACTCGCTACTCATCGGCCTACGAGCTCGAGGGTGCCGTCAAACGTGCCGACCTGGTGATTGGGGCCGTCCTG
GTGCCAGGCGCCAAGGCACCCAAATTAGTCTCGAATTCACTTGTCGCGCATATGAAACCAGGTGCGGTACTGGTGGATAT
AGCCATCGACCAGGGCGGCTGTTTCGAAGGCTCACGACCGACCACCTACGACCACCCGACGTTCGCCGTGCACGACACGC
TGTTTTACTGCGTGGCGAACATGCCCGCCTCGGTGCCGAAGACGTCGACCTACGCGCTGACCAACGCGACGATGCCGTAT
GTGCTCGAGCTTGCCGACCATGGCTGGCGGGCGGCGTGCCGGTCGAATCCGGCACTAGCCAAAGGTCTTTCGACGCACGA
AGGGGCGTTACTGTCCGAACGGGTGGCCACCGACCTGGGGGTGCCGTTCACCGAGCCCGCCAGCGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
TTATCACGCATATGGCCTCGAAAAAGAATTATCATCAGCAATCTTGCAGATTAATCGAACTTTCTTCACACTGAAGCGTA
CAGTATCGAGAGGGGTAATC

Downstream 100 bases:

>100_bases
CTCTCGGCCGCTCGTTACGCCGAGCACACGTCGGGAGTAAGGGAAGCGATGATGTCGGCCGCGGGTCCCGGCCGGGTCTT
CCGGTGCGCCGATCCCGCCC

Product: secreted L-alanine dehydrogenase ald (40 kDa antigen)

Products: NA

Alternate protein names: 40 kDa antigen; TB43

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRVGIPTETKNNEFRVAITPAGVAELTRRGHEVLIQAGAGEGSAITDADFKAAGAQLVGTADQVWADADLLLKVKEPIAA
EYGRLRHGQILFTFLHLAASRACTDALLDSGTTSIAYETVQTADGALPLLAPMSEVAGRLAAQVGAYHLMRTQGGRGVLM
GGVPGVEPADVVVIGAGTAGYNAARIANGMGATVTVLDINIDKLRQLDAEFCGRIHTRYSSAYELEGAVKRADLVIGAVL
VPGAKAPKLVSNSLVAHMKPGAVLVDIAIDQGGCFEGSRPTTYDHPTFAVHDTLFYCVANMPASVPKTSTYALTNATMPY
VLELADHGWRAACRSNPALAKGLSTHEGALLSERVATDLGVPFTEPASVLA

Sequences:

>Translated_371_residues
MRVGIPTETKNNEFRVAITPAGVAELTRRGHEVLIQAGAGEGSAITDADFKAAGAQLVGTADQVWADADLLLKVKEPIAA
EYGRLRHGQILFTFLHLAASRACTDALLDSGTTSIAYETVQTADGALPLLAPMSEVAGRLAAQVGAYHLMRTQGGRGVLM
GGVPGVEPADVVVIGAGTAGYNAARIANGMGATVTVLDINIDKLRQLDAEFCGRIHTRYSSAYELEGAVKRADLVIGAVL
VPGAKAPKLVSNSLVAHMKPGAVLVDIAIDQGGCFEGSRPTTYDHPTFAVHDTLFYCVANMPASVPKTSTYALTNATMPY
VLELADHGWRAACRSNPALAKGLSTHEGALLSERVATDLGVPFTEPASVLA
>Mature_371_residues
MRVGIPTETKNNEFRVAITPAGVAELTRRGHEVLIQAGAGEGSAITDADFKAAGAQLVGTADQVWADADLLLKVKEPIAA
EYGRLRHGQILFTFLHLAASRACTDALLDSGTTSIAYETVQTADGALPLLAPMSEVAGRLAAQVGAYHLMRTQGGRGVLM
GGVPGVEPADVVVIGAGTAGYNAARIANGMGATVTVLDINIDKLRQLDAEFCGRIHTRYSSAYELEGAVKRADLVIGAVL
VPGAKAPKLVSNSLVAHMKPGAVLVDIAIDQGGCFEGSRPTTYDHPTFAVHDTLFYCVANMPASVPKTSTYALTNATMPY
VLELADHGWRAACRSNPALAKGLSTHEGALLSERVATDLGVPFTEPASVLA

Specific function: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Secreted

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family

Homologues:

Organism=Homo sapiens, GI122939155, Length=308, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI122939153, Length=308, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-32,
Organism=Escherichia coli, GI1787887, Length=302, Percent_Identity=32.4503311258278, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17550456, Length=346, Percent_Identity=29.4797687861272, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHA_MYCTU (P30234)

Other databases:

- EMBL:   X63069
- EMBL:   U92472
- EMBL:   BX842580
- EMBL:   AE000516
- PIR:   C70883
- RefSeq:   NP_217296.1
- RefSeq:   NP_337355.1
- PDB:   2VHV
- PDB:   2VHW
- PDB:   2VHX
- PDB:   2VHY
- PDB:   2VHZ
- PDB:   2VOE
- PDB:   2VOJ
- PDBsum:   2VHV
- PDBsum:   2VHW
- PDBsum:   2VHX
- PDBsum:   2VHY
- PDBsum:   2VHZ
- PDBsum:   2VOE
- PDBsum:   2VOJ
- ProteinModelPortal:   P30234
- SMR:   P30234
- EnsemblBacteria:   EBMYCT00000000847
- EnsemblBacteria:   EBMYCT00000070854
- GeneID:   888493
- GeneID:   925435
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2850
- KEGG:   mtu:Rv2780
- TIGR:   MT2850
- TubercuList:   Rv2780
- GeneTree:   EBGT00050000016024
- HOGENOM:   HBG289735
- OMA:   NATIAHA
- ProtClustDB:   CLSK792072
- BRENDA:   1.4.1.1
- GO:   GO:0005829
- GO:   GO:0005488
- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141
- PIRSF:   PIRSF000183
- TIGRFAMs:   TIGR00518

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N

EC number: =1.4.1.1

Molecular weight: Translated: 38714; Mature: 38714

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00836 ALADH_PNT_1; PS00837 ALADH_PNT_2

Important sites: ACT_SITE 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGIPTETKNNEFRVAITPAGVAELTRRGHEVLIQAGAGEGSAITDADFKAAGAQLVGT
CCCCCCCCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCCCCCEECCCCHHHCCCEEECC
ADQVWADADLLLKVKEPIAAEYGRLRHGQILFTFLHLAASRACTDALLDSGTTSIAYETV
HHHHHCCCHHEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEH
QTADGALPLLAPMSEVAGRLAAQVGAYHLMRTQGGRGVLMGGVPGVEPADVVVIGAGTAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEEECCCCC
YNAARIANGMGATVTVLDINIDKLRQLDAEFCGRIHTRYSSAYELEGAVKRADLVIGAVL
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEE
VPGAKAPKLVSNSLVAHMKPGAVLVDIAIDQGGCFEGSRPTTYDHPTFAVHDTLFYCVAN
CCCCCCCHHHHCCHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPASVPKTSTYALTNATMPYVLELADHGWRAACRSNPALAKGLSTHEGALLSERVATDLG
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC
VPFTEPASVLA
CCCCCCHHHCC
>Mature Secondary Structure
MRVGIPTETKNNEFRVAITPAGVAELTRRGHEVLIQAGAGEGSAITDADFKAAGAQLVGT
CCCCCCCCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCCCCCEECCCCHHHCCCEEECC
ADQVWADADLLLKVKEPIAAEYGRLRHGQILFTFLHLAASRACTDALLDSGTTSIAYETV
HHHHHCCCHHEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEH
QTADGALPLLAPMSEVAGRLAAQVGAYHLMRTQGGRGVLMGGVPGVEPADVVVIGAGTAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEEECCCCC
YNAARIANGMGATVTVLDINIDKLRQLDAEFCGRIHTRYSSAYELEGAVKRADLVIGAVL
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEE
VPGAKAPKLVSNSLVAHMKPGAVLVDIAIDQGGCFEGSRPTTYDHPTFAVHDTLFYCVAN
CCCCCCCHHHHCCHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPASVPKTSTYALTNATMPYVLELADHGWRAACRSNPALAKGLSTHEGALLSERVATDLG
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC
VPFTEPASVLA
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587598; 9634230; 12218036; 7822223