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The map label for this gene is ophA1 [H]

Identifier: 148823964

GI number: 148823964

Start: 3095329

End: 3096420

Strand: Reverse

Name: ophA1 [H]

Synonym: TBFG_12789

Alternate gene names: 148823964

Gene position: 3096420-3095329 (Counterclockwise)

Preceding gene: 148823965

Following gene: 148823962

Centisome position: 69.98

GC content: 67.95

Gene sequence:

>1092_bases
ATGGCGGAGCGCACCGTGCCAGAGACCAGCTGGGCCAGTAGGCCCGCCGACCTTTACGGTCGCCGCAGCCGGGACCGCTT
CTTCACAGCGCTGTGGGGTGTACGAGCGTTATTGGGAGGATTGGGCGCGGCCTCGCGGTGGGAACCGTCGCGGGTGCAGC
CGGTGCGGCGCACGAATCCCGCGGTGGTTACCAAGCGCGAGCTGGTCGCTCCCGACGTGGTGGCGTTGACGTTGGCTGAC
CCCGGTGGCGGATTGCTCCCGGCCTGGTCGCCCGGTGGGCACATCGACGTGCAGCTGCCCTCGGGGCGGCGCCGACAGTA
CTCGTTGTGCGGTGTGCCCGGACGGCGTACGGACTACCGGATCGCCATTCGCAGAATCGCGGACGGCGGCGGCGGTTCGA
TCGAGATGCACGAGGCCTTCGACGTAGGTGACACGTGCGAGTTCGAAGGTCCCCGCAACGCCTTCCATCTCGGTCTGGCC
GAGCGTGACGTGCTTTTCGTGATCGGCGGTATCGGGGTGACGCCCATCCTGCCGATGATCCGGGCCGCCGAACAGCGCGG
AATCGACTGGCGCGCAATCTATGCCGGTCGCGGTCGGGAGTACATGCCGTTCCTGGACGAGGTAGTGGCGGTGGCACCCG
GCCGGGTCACGGTGTGGGCCGACGACGAGCACGGTCGTTTTGCGTCTGTCGATGAACTGTTGGCCGGCGCAGGGCCGACG
ACGGCCGTTTACGTGTGCGGGCCGCCCGGGATGTTAGAAGCGGTGCGCGTGGCCCGCAATCAACACGCCGACGCGCCGCT
GCATTACGAGCGATTCAGCCCGCCGCCGGTTGTCGACGGAGTTCCGTTCGAGCTGGAGCTCGCACGGTCGAGGCGAGTGC
TCCGAGTTCCGGCGAACCGGTCGGCGCTGGACGTCATGCTCGACTGGGACCCGACGACGGCATACTCCTGCCAGCAGGGC
TTCTGCGGGACCTGCAAGGTGAGAGTCCTTGCCGGGCAGGTCGACCGCCGCGGCCGCATCATCGAAGGCGATAACGAGAT
GCTGGTCTGTGTGTCGCGGGCCGTGAGCGGTCGGGTGGTTATCGACGCCTGA

Upstream 100 bases:

>100_bases
TTTCACCCGTCCCAGCTGGGCGGGCTGGGGGCGGCCGTGGACTACCTGGCCGTCTCGCCCGCCGCGCGAGCGTCGCACTG
ATCGGTGACGATCCGTAGGA

Downstream 100 bases:

>100_bases
TGCTTACCCGGGCGGGGCGGATCGATAAAGCCGGTAGCCCACATGGGGCACGTTCACACCTGACAGTGGTTTCCACGTGA
AAGTGCGGCCGTCAATGTGA

Product: oxidoreductase

Products: NA

Alternate protein names: PDR [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAERTVPETSWASRPADLYGRRSRDRFFTALWGVRALLGGLGAASRWEPSRVQPVRRTNPAVVTKRELVAPDVVALTLAD
PGGGLLPAWSPGGHIDVQLPSGRRRQYSLCGVPGRRTDYRIAIRRIADGGGGSIEMHEAFDVGDTCEFEGPRNAFHLGLA
ERDVLFVIGGIGVTPILPMIRAAEQRGIDWRAIYAGRGREYMPFLDEVVAVAPGRVTVWADDEHGRFASVDELLAGAGPT
TAVYVCGPPGMLEAVRVARNQHADAPLHYERFSPPPVVDGVPFELELARSRRVLRVPANRSALDVMLDWDPTTAYSCQQG
FCGTCKVRVLAGQVDRRGRIIEGDNEMLVCVSRAVSGRVVIDA

Sequences:

>Translated_363_residues
MAERTVPETSWASRPADLYGRRSRDRFFTALWGVRALLGGLGAASRWEPSRVQPVRRTNPAVVTKRELVAPDVVALTLAD
PGGGLLPAWSPGGHIDVQLPSGRRRQYSLCGVPGRRTDYRIAIRRIADGGGGSIEMHEAFDVGDTCEFEGPRNAFHLGLA
ERDVLFVIGGIGVTPILPMIRAAEQRGIDWRAIYAGRGREYMPFLDEVVAVAPGRVTVWADDEHGRFASVDELLAGAGPT
TAVYVCGPPGMLEAVRVARNQHADAPLHYERFSPPPVVDGVPFELELARSRRVLRVPANRSALDVMLDWDPTTAYSCQQG
FCGTCKVRVLAGQVDRRGRIIEGDNEMLVCVSRAVSGRVVIDA
>Mature_362_residues
AERTVPETSWASRPADLYGRRSRDRFFTALWGVRALLGGLGAASRWEPSRVQPVRRTNPAVVTKRELVAPDVVALTLADP
GGGLLPAWSPGGHIDVQLPSGRRRQYSLCGVPGRRTDYRIAIRRIADGGGGSIEMHEAFDVGDTCEFEGPRNAFHLGLAE
RDVLFVIGGIGVTPILPMIRAAEQRGIDWRAIYAGRGREYMPFLDEVVAVAPGRVTVWADDEHGRFASVDELLAGAGPTT
AVYVCGPPGMLEAVRVARNQHADAPLHYERFSPPPVVDGVPFELELARSRRVLRVPANRSALDVMLDWDPTTAYSCQQGF
CGTCKVRVLAGQVDRRGRIIEGDNEMLVCVSRAVSGRVVIDA

Specific function: Component of the electron transfer chain involved in pyridine nucleotide-dependent dihydroxylation of phthalate. Utilizes FMN to mediate electron transfer from the two-electron donor, NADH, to the one-electron acceptor, (2Fe-2S) [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=311, Percent_Identity=33.4405144694534, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788903, Length=234, Percent_Identity=29.0598290598291, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39558; Mature: 39426

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2 ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERTVPETSWASRPADLYGRRSRDRFFTALWGVRALLGGLGAASRWEPSRVQPVRRTNP
CCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHCCCC
AVVTKRELVAPDVVALTLADPGGGLLPAWSPGGHIDVQLPSGRRRQYSLCGVPGRRTDYR
CEEEHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCE
IAIRRIADGGGGSIEMHEAFDVGDTCEFEGPRNAFHLGLAERDVLFVIGGIGVTPILPMI
EEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHH
RAAEQRGIDWRAIYAGRGREYMPFLDEVVAVAPGRVTVWADDEHGRFASVDELLAGAGPT
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCC
TAVYVCGPPGMLEAVRVARNQHADAPLHYERFSPPPVVDGVPFELELARSRRVLRVPANR
EEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEECCCCEEEECCCCC
SALDVMLDWDPTTAYSCQQGFCGTCKVRVLAGQVDRRGRIIEGDNEMLVCVSRAVSGRVV
CEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEE
IDA
EEC
>Mature Secondary Structure 
AERTVPETSWASRPADLYGRRSRDRFFTALWGVRALLGGLGAASRWEPSRVQPVRRTNP
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHCCCC
AVVTKRELVAPDVVALTLADPGGGLLPAWSPGGHIDVQLPSGRRRQYSLCGVPGRRTDYR
CEEEHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCE
IAIRRIADGGGGSIEMHEAFDVGDTCEFEGPRNAFHLGLAERDVLFVIGGIGVTPILPMI
EEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHH
RAAEQRGIDWRAIYAGRGREYMPFLDEVVAVAPGRVTVWADDEHGRFASVDELLAGAGPT
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCC
TAVYVCGPPGMLEAVRVARNQHADAPLHYERFSPPPVVDGVPFELELARSRRVLRVPANR
EEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEECCCCEEEECCCCC
SALDVMLDWDPTTAYSCQQGFCGTCKVRVLAGQVDRRGRIIEGDNEMLVCVSRAVSGRVV
CEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEE
IDA
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9851995; 1280857 [H]