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The map label for this gene is thyA [H]

Identifier: 148823952

GI number: 148823952

Start: 3085635

End: 3086426

Strand: Reverse

Name: thyA [H]

Synonym: TBFG_12777

Alternate gene names: 148823952

Gene position: 3086426-3085635 (Counterclockwise)

Preceding gene: 148823954

Following gene: 148823951

Centisome position: 69.76

GC content: 60.1

Gene sequence:

>792_bases
GTGACGCCATACGAGGACCTGCTGCGCTTCGTGCTCGAAACGGGTACGCCCAAATCCGACCGCACCGGCACCGGAACCCG
CAGCCTGTTCGGCCAGCAGATGCGCTATGATTTGTCGGCCGGTTTCCCGCTGCTCACTACCAAGAAAGTCCATTTCAAAT
CGGTAGCCTACGAGCTGCTGTGGTTTTTGCGCGGCGATTCCAATATCGGTTGGCTGCACGAGCACGGAGTCACCATCTGG
GACGAATGGGCAAGTGATACAGGCGAACTCGGGCCGATCTACGGTGTACAATGGCGATCGTGGCCGGCTCCATCCGGTGA
GCACATCGACCAGATCAGCGCGGCGCTGGATTTGCTGCGCACCGATCCCGATTCCCGGCGCATCATCGTGTCGGCCTGGA
ACGTCGGCGAAATCGAGCGGATGGCGCTGCCGCCCTGTCATGCGTTCTTCCAGTTCTACGTCGCCGATGGCCGGCTGAGC
TGTCAGCTCTACCAACGCAGCGCCGACCTGTTTCTGGGTGTGCCGTTCAACATCGCCAGCTATGCGTTGCTCACCCACAT
GATGGCCGCCCAGGCCGGCTTGTCGGTCGGCGAGTTCATCTGGGCCGGTGGCGACTGCCACATCTACGACAATCACGTCG
AGCAAGTACGGCTGCAGCTCAGCCGCGAGCCGCGGCCATATCCGAAACTACTTCTAGCCGACCGGGATTCAATCTTCGAG
TACACCTATGAAGACATCGTTGTGAAGAACTACGATCCGCATCCGGCGATCAAAGCTCCAGTCGCGGTATGA

Upstream 100 bases:

>100_bases
TCGCCGCGCTTGCGATCGCCCGCTGGGTTTGTTTGGCGGCGAGTCGCTGCGGCGGCTTCGCCGCGCTTGCGATCGCCGCT
AGGCTGACCGCGTGTCAATC

Downstream 100 bases:

>100_bases
GGCGCGCCGACGACAATGAAGTCGGCTCGGACAGCCTTCAAGCCGGAATCAAGGAGTGACGCGTGTGACGATGGTGGGGC
TGATCTGGGCTCAAGCGACA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTPYEDLLRFVLETGTPKSDRTGTGTRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELLWFLRGDSNIGWLHEHGVTIW
DEWASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLS
CQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWAGGDCHIYDNHVEQVRLQLSREPRPYPKLLLADRDSIFE
YTYEDIVVKNYDPHPAIKAPVAV

Sequences:

>Translated_263_residues
MTPYEDLLRFVLETGTPKSDRTGTGTRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELLWFLRGDSNIGWLHEHGVTIW
DEWASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLS
CQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWAGGDCHIYDNHVEQVRLQLSREPRPYPKLLLADRDSIFE
YTYEDIVVKNYDPHPAIKAPVAV
>Mature_262_residues
TPYEDLLRFVLETGTPKSDRTGTGTRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELLWFLRGDSNIGWLHEHGVTIWD
EWASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLSC
QLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWAGGDCHIYDNHVEQVRLQLSREPRPYPKLLLADRDSIFEY
TYEDIVVKNYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=49.8220640569395, Blast_Score=274, Evalue=7e-74,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.9090909090909, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71993377, Length=275, Percent_Identity=45.8181818181818, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=42.6086956521739, Blast_Score=188, Evalue=6e-49,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=49.1166077738516, Blast_Score=273, Evalue=6e-74,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 29823; Mature: 29692

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYEDLLRFVLETGTPKSDRTGTGTRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELL
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
WFLRGDSNIGWLHEHGVTIWDEWASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLR
HHHCCCCCCCEEECCCCEEEEHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
TDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLSCQLYQRSADLFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMMAAQAGLSVGEFIWAGGDCHIYDNHVEQVRLQLSREPRPYPKLLLADRDSIFE
HHHHHHHHHHHHCCCHHHEEEECCEEEEECCHHHHHHHHHCCCCCCCCCEEEECCHHHHH
YTYEDIVVKNYDPHPAIKAPVAV
HHHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TPYEDLLRFVLETGTPKSDRTGTGTRSLFGQQMRYDLSAGFPLLTTKKVHFKSVAYELL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
WFLRGDSNIGWLHEHGVTIWDEWASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLR
HHHCCCCCCCEEECCCCEEEEHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
TDPDSRRIIVSAWNVGEIERMALPPCHAFFQFYVADGRLSCQLYQRSADLFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMMAAQAGLSVGEFIWAGGDCHIYDNHVEQVRLQLSREPRPYPKLLLADRDSIFE
HHHHHHHHHHHHCCCHHHEEEECCEEEEECCHHHHHHHHHCCCCCCCCCEEEECCHHHHH
YTYEDIVVKNYDPHPAIKAPVAV
HHHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA