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The map label for this gene is sigB [H]

Identifier: 148823899

GI number: 148823899

Start: 3034350

End: 3035387

Strand: Direct

Name: sigB [H]

Synonym: TBFG_12724

Alternate gene names: 148823899

Gene position: 3034350-3035387 (Clockwise)

Preceding gene: 148823897

Following gene: 148823900

Centisome position: 68.58

GC content: 63.1

Gene sequence:

>1038_bases
GTGGGAACTCAAAGTCCGGCTTTGTCGTTAAACCCCATGACAGTGCAAGCCGATCGGGAGGTCGCTATGGCCGATGCACC
CACAAGGGCCACCACAAGCCGGGTTGACAGCGATCTGGATGCTCAAAGCCCCGCGGCGGACCTCGTGCGCGTCTATCTGA
ACGGCATCGGCAAGACGGCGTTGCTCAACGCCGCCGGTGAAGTCGAACTGGCCAAGCGCATAGAAGCCGGGTTGTATGCC
GAGCATCTGCTGGAAACCCGGAAGCGCCTCGGCGAGAACCGAAAACGCGACCTGGCGGCCGTGGTGCGTGATGGCGAGGC
GGCGCGCCGCCACCTGCTGGAAGCAAACCTGCGGCTGGTGGTATCGCTGGCCAAGCGCTACACGGGTCGGGGCATGCCGT
TGCTGGACCTCATCCAGGAGGGCAACCTGGGTCTGATCCGAGCGATGGAGAAGTTCGACTACACAAAGGGATTCAAGTTC
TCAACGTATGCCACGTGGTGGATCCGCCAGGCCATCACCCGCGGAATGGCCGACCAGAGCCGCACCATCCGCCTGCCCGT
ACACCTGGTTGAGCAGGTCAACAAGCTGGCGCGGATCAAGCGGGAGATGCACCAGCATCTGGGTCGCGAAGCCACCGATG
AGGAGCTCGCCGCCGAATCCGGCATTCCAATCGACAAGATCAACGACCTGCTGGAACACAGTCGCGACCCGGTGAGTCTG
GATATGCCGGTCGGCTCCGAGGAGGAGGCCCCTTTGGGCGATTTCATCGAGGACGCCGAAGCCATGTCCGCGGAGAACGC
GGTCATCGCCGAACTGTTACACACCGACATCCGCAGCGTGCTGGCCACTCTCGACGAGCGTGAGCACCAGGTGATCCGGC
TGCGCTTCGGCCTGGATGACGGCCAACCACGCACCCTGGATCAAATCGGCAAACTATTCGGGCTGTCCCGTGAGCGGGTT
CGTCAGATCGAGCGCGACGTGATGAGTAAGCTGCGGCACGGTGAGCGGGCGGATCGGCTGCGGTCGTACGCCAGCTGA

Upstream 100 bases:

>100_bases
GGCCGGCGTTCCGGGTTGTCGGCCACTGGCCACACTTCTCAGGACTTTCTCAGGTCTTCGGCAGATTCCTGCACGTCACA
GGGCGTCAGATCACTGCTGG

Downstream 100 bases:

>100_bases
AGCTGGACATCCTGAGCCAGGTAGCAGACGGTATGCCCGCCGCGCCAGCGGCGGGCATACCGCTGCGGTGGGGCGGCGGG
CAACCATTTTCGCAGCTGGC

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MGTQSPALSLNPMTVQADREVAMADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYA
EHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKF
STYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGIPIDKINDLLEHSRDPVSL
DMPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERV
RQIERDVMSKLRHGERADRLRSYAS

Sequences:

>Translated_345_residues
MGTQSPALSLNPMTVQADREVAMADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYA
EHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKF
STYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGIPIDKINDLLEHSRDPVSL
DMPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERV
RQIERDVMSKLRHGERADRLRSYAS
>Mature_344_residues
GTQSPALSLNPMTVQADREVAMADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYAE
HLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFS
TYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGIPIDKINDLLEHSRDPVSLD
MPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVR
QIERDVMSKLRHGERADRLRSYAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=48.7084870848708, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789098, Length=317, Percent_Identity=37.8548895899054, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=29.8319327731092, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38643; Mature: 38511

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQSPALSLNPMTVQADREVAMADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTA
CCCCCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHH
LLNAAGEVELAKRIEAGLYAEHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
VSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQS
HHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
RTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGIPIDKINDLLEHSRDPVSL
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEE
DMPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDD
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
GQPRTLDQIGKLFGLSRERVRQIERDVMSKLRHGERADRLRSYAS
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
GTQSPALSLNPMTVQADREVAMADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTA
CCCCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHH
LLNAAGEVELAKRIEAGLYAEHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
VSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQS
HHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
RTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGIPIDKINDLLEHSRDPVSL
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEE
DMPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDD
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
GQPRTLDQIGKLFGLSRERVRQIERDVMSKLRHGERADRLRSYAS
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]