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The map label for this gene is arsB [H]

Identifier: 148823836

GI number: 148823836

Start: 2980227

End: 2981723

Strand: Direct

Name: arsB [H]

Synonym: TBFG_12661

Alternate gene names: 148823836

Gene position: 2980227-2981723 (Clockwise)

Preceding gene: 148823835

Following gene: 148823837

Centisome position: 67.36

GC content: 65.93

Gene sequence:

>1497_bases
ATGACGGAGACGGTCACCCGCACCGCCGCCCCGGCGGTGGTGGGCAAACTCTCGACGCTGGACCGCTTCTTGCCGGTGTG
GATCGGGTCGGCAATGGCCGCCGGGCTACTACTGGGCCGGTGGATTCCCGGCCTGCACACCGCCCTAGAAGGGGTTCAGC
TCGACGGGATTTCGCTGCCGATCGCGCTAGGCCTGCTGATCATGATGTATCCGGTGCTGGCCAAGGTGCGCTACGACCGC
CTCGACACCGTCACCGGTGACCGCAAGCTGCTACTCAGCTCGCTGCTGCTGAACTGGGTACTGGGCCCGGCGTTGATGTT
CGCGCTGGCTTGGCTGCTACTGGCGGATCTGCCCGAGTACCGCACCGGGCTGATCATCGTGGGCCTGGCTCGCTGCATCG
CCATGGTGATCATCTGGAACGACCTGGCCTGCGGGGATCGCGAAGCCGCCGCCGTGCTCGTCGCGTTGAACTCGATCTTT
CAGGTGGCCATGTTCGCCGCGCTCGGCTGGTTCTACCTGTCGGTGCTACCGGGTTGGCTGGGCCTCGAGCAGACCACCAT
CGCCACATCCCCGTGGCAGATCGCCAAGTCGGTGCTGATCTTCCTCGGCATCCCGCTGCTGGCCGGCTACCTGTCGCGGC
GGATCGGCGAAAAGACCAAGGGCCGCAACTGGTATGAATCCCGCTTCCTGCCCAAGGTGGGACCGTGGGCGCTCTACGGT
TTGCTGTTCACCATCGTGATTCTCTTTGCGCTGCAAGGAGATCAGATCACCGGCCGACCGCTGGACGTCGCACGCATTGC
GCTGCCGCTGCTGGCCTACTTCGCCATCATGTGGGTAGGCGGCTACCTACTGGGGGCGGCGCTGCGGCTAGGGTATCGGC
GCACCACCACGCTGGCGTTCACCGCCGCGAGCAACAACTTCGAGCTGGCCATCGCGGTGGCCATCGCCACCTACGGCGCC
ACCTCCGGGCAAGCCCTGGCCGGAGTCGTCGGGCCCCTGATCGAGGTACCCGTCCTGGTGGGGTTGGTCTATGTGTCCCT
GGCGCTGCGCAACCGCCTCGCCGGTCCCAACGCGACCCACGATGCCGACAAACCCAGCGTCCTATTCGTCTGTGTGCACA
ACGCCGGACGTTCCCAGATGGCCGCCGGGCTATTGACCCACTTGGCCGGTGACCGCATCGAAGTCCGTTCGGCCGGAACC
GAGCCCGCCGGTCAGGTCAATCCGACGGCTGTGGCCGCGATGGCCGAAATGGGCATCGATATCACCGCCAATGCCCCCAC
ATTGCTCACCGGCGGGCAGGTCCAGTCCAGCGACGTCGTCATCACGATGGGCTGCGGCGATGCCTGCCCTTACTTCCCGG
GTGTCTCCTACCGCAACTGGAAACTACCCGATCCCGCCGGCCAGCCCCTCGACGTTGTGCGCATGATCCGCGACGACATC
GCAGACCGCGTCCAAGCCCTGATCGCCGAGCTGCTGGCCACCGCCAAGACCAGATAG

Upstream 100 bases:

>100_bases
CCTCGTGGGTGTACTACGCCGTGGTCCCCGAGGCGCTGACCGTGTTGTCGAACCTGCTCAGCGTGCATGCCGATGCCGCA
CCCGCCCTGGGGGCACCGGC

Downstream 100 bases:

>100_bases
CGTGTGCCACGCTCGGTGCTGCGCCGATACGTGAGGTCCCGGCTGGGATCGGATTTTCCGCGTGTACGGCGGCTAGGCAC
CAGCGGATCGCATTTGTACT

Product: arsenic-transport integral membrane protein arsC

Products: arsenite; glutaredoxin disulfide

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTETVTRTAAPAVVGKLSTLDRFLPVWIGSAMAAGLLLGRWIPGLHTALEGVQLDGISLPIALGLLIMMYPVLAKVRYDR
LDTVTGDRKLLLSSLLLNWVLGPALMFALAWLLLADLPEYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIF
QVAMFAALGWFYLSVLPGWLGLEQTTIATSPWQIAKSVLIFLGIPLLAGYLSRRIGEKTKGRNWYESRFLPKVGPWALYG
LLFTIVILFALQGDQITGRPLDVARIALPLLAYFAIMWVGGYLLGAALRLGYRRTTTLAFTAASNNFELAIAVAIATYGA
TSGQALAGVVGPLIEVPVLVGLVYVSLALRNRLAGPNATHDADKPSVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGT
EPAGQVNPTAVAAMAEMGIDITANAPTLLTGGQVQSSDVVITMGCGDACPYFPGVSYRNWKLPDPAGQPLDVVRMIRDDI
ADRVQALIAELLATAKTR

Sequences:

>Translated_498_residues
MTETVTRTAAPAVVGKLSTLDRFLPVWIGSAMAAGLLLGRWIPGLHTALEGVQLDGISLPIALGLLIMMYPVLAKVRYDR
LDTVTGDRKLLLSSLLLNWVLGPALMFALAWLLLADLPEYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIF
QVAMFAALGWFYLSVLPGWLGLEQTTIATSPWQIAKSVLIFLGIPLLAGYLSRRIGEKTKGRNWYESRFLPKVGPWALYG
LLFTIVILFALQGDQITGRPLDVARIALPLLAYFAIMWVGGYLLGAALRLGYRRTTTLAFTAASNNFELAIAVAIATYGA
TSGQALAGVVGPLIEVPVLVGLVYVSLALRNRLAGPNATHDADKPSVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGT
EPAGQVNPTAVAAMAEMGIDITANAPTLLTGGQVQSSDVVITMGCGDACPYFPGVSYRNWKLPDPAGQPLDVVRMIRDDI
ADRVQALIAELLATAKTR
>Mature_497_residues
TETVTRTAAPAVVGKLSTLDRFLPVWIGSAMAAGLLLGRWIPGLHTALEGVQLDGISLPIALGLLIMMYPVLAKVRYDRL
DTVTGDRKLLLSSLLLNWVLGPALMFALAWLLLADLPEYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIFQ
VAMFAALGWFYLSVLPGWLGLEQTTIATSPWQIAKSVLIFLGIPLLAGYLSRRIGEKTKGRNWYESRFLPKVGPWALYGL
LFTIVILFALQGDQITGRPLDVARIALPLLAYFAIMWVGGYLLGAALRLGYRRTTTLAFTAASNNFELAIAVAIATYGAT
SGQALAGVVGPLIEVPVLVGLVYVSLALRNRLAGPNATHDADKPSVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGTE
PAGQVNPTAVAAMAEMGIDITANAPTLLTGGQVQSSDVVITMGCGDACPYFPGVSYRNWKLPDPAGQPLDVVRMIRDDIA
DRVQALIAELLATAKTR

Specific function: Seems to confer resistance to arsenite by allowing cells to extrude this compound. Could be part of an arsenite extrusion pump [H]

COG id: COG0798

COG function: function code P; Arsenite efflux pump ACR3 and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ACR3 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325459, Length=380, Percent_Identity=35, Blast_Score=192, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004706
- InterPro:   IPR002657 [H]

Pfam domain/function: PF01758 SBF [H]

EC number: 1.20.4.1

Molecular weight: Translated: 53226; Mature: 53095

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETVTRTAAPAVVGKLSTLDRFLPVWIGSAMAAGLLLGRWIPGLHTALEGVQLDGISLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCHHH
IALGLLIMMYPVLAKVRYDRLDTVTGDRKLLLSSLLLNWVLGPALMFALAWLLLADLPEY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIFQVAMFAALGWFYLSVLPGWL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEQTTIATSPWQIAKSVLIFLGIPLLAGYLSRRIGEKTKGRNWYESRFLPKVGPWALYG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHH
LLFTIVILFALQGDQITGRPLDVARIALPLLAYFAIMWVGGYLLGAALRLGYRRTTTLAF
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TAASNNFELAIAVAIATYGATSGQALAGVVGPLIEVPVLVGLVYVSLALRNRLAGPNATH
EEECCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DADKPSVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGTEPAGQVNPTAVAAMAEMGID
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCE
ITANAPTLLTGGQVQSSDVVITMGCGDACPYFPGVSYRNWKLPDPAGQPLDVVRMIRDDI
EECCCCEEEECCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ADRVQALIAELLATAKTR
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TETVTRTAAPAVVGKLSTLDRFLPVWIGSAMAAGLLLGRWIPGLHTALEGVQLDGISLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCHHH
IALGLLIMMYPVLAKVRYDRLDTVTGDRKLLLSSLLLNWVLGPALMFALAWLLLADLPEY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIFQVAMFAALGWFYLSVLPGWL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEQTTIATSPWQIAKSVLIFLGIPLLAGYLSRRIGEKTKGRNWYESRFLPKVGPWALYG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHH
LLFTIVILFALQGDQITGRPLDVARIALPLLAYFAIMWVGGYLLGAALRLGYRRTTTLAF
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TAASNNFELAIAVAIATYGATSGQALAGVVGPLIEVPVLVGLVYVSLALRNRLAGPNATH
EEECCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DADKPSVLFVCVHNAGRSQMAAGLLTHLAGDRIEVRSAGTEPAGQVNPTAVAAMAEMGID
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCE
ITANAPTLLTGGQVQSSDVVITMGCGDACPYFPGVSYRNWKLPDPAGQPLDVVRMIRDDI
EECCCCEEEECCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ADRVQALIAELLATAKTR
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arsenate; glutaredoxin

Specific reaction: arsenate + glutaredoxin = arsenite + glutaredoxin disulfide

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7704261; 8969508; 9384377; 7489895; 9537360 [H]