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The map label for this gene is bkdB [H]

Identifier: 148823692

GI number: 148823692

Start: 2822076

End: 2823257

Strand: Reverse

Name: bkdB [H]

Synonym: TBFG_12517

Alternate gene names: 148823692

Gene position: 2823257-2822076 (Counterclockwise)

Preceding gene: 148823693

Following gene: 148823688

Centisome position: 63.81

GC content: 65.48

Gene sequence:

>1182_bases
ATGAGCGGTGAGGACAGCATCAGGTCTTTCCCGGTGCCCGACCTCGGCGAGGGACTGCAGGAAGTGACGGTGACGTGTTG
GAGCGTCGCCGTCGGCGACGATGTGGAGATCAACCAGACGCTGTGTTCGGTGGAGACCGCCAAGGCCGAGGTCGAAATCC
CCAGCCCGTATGCCGGCCGGATCGTCGAGTTAGGCGGCGCCGAAGGCGATGTGCTCAAAGTGGGCGCGGAGCTAGTTCGG
ATCGACACCGGGCCCACGGCAGTTGCGCAGCCTAACGGTGAAGGAGCGGTCCCCACGTTGGTCGGCTACGGTGCCGACGC
CGCGATCGAAACCAGTAGACGGACAAGCCGGCCGCTGGCGGCACCGGTAGTGCGCAAGCTGGCCAAAGAGTTGGCGGTCG
ACCTGGCCGCATTGCAGCGTGGGTCGGGCGCCGGCGGTGTGATCACCCGGGCCGATGTGCTGGCCGCTGCTCGAGGCGGC
GTCGGAGCCGGGCCGGACGTGCGGCCGGTCCACGGCGTGCACGCGCGGATGGCCGAAAAAATGACGTTGTCCCACAAGGA
GATTCCGACCGCAAAGGCCAGCGTTGAGGTAATTTGCGCCGAACTGCTGCGGCTGCGCGACCGGTTCGTTTCGGCGGCGC
CCGAGATTACACCGTTCGCGCTGACGCTGCGGCTGCTGGTTATTGCATTGAAACACAACGTAATTCTCAACTCGACGTGG
GTCGACTCGGGCGAAGGCCCGCAAGTACACGTGCATCGCGGTGTGCATCTGGGGTTCGGCGCGGCCACTGAGCGTGGATT
GCTGGTGCCGGTGGTGACCGACGCCCAGGACAAGAACACCCGCGAACTTGCCTCCCGCGTAGCGGAATTAATCACCGGCG
CACGTGAAGGCACTCTCACACCCGCGGAGCTGCGCGGTTCGACGTTCACGGTGTCGAACTTCGGGGCGCTGGGAGTCGAC
GACGGCGTGCCGGTGATCAACCATCCCGAAGCGGCGATCCTGGGTCTGGGGGCGATCAAGCCGCGCCCGGTGGTCGTCGG
CGGCGAGGTTGTCGCACGGCCGACGATGACGTTGACTTGTGTGTTCGACCACCGCGTTGTCGATGGTGCTCAGGTGGCCC
AGTTCATGTGTGAGCTGCGGGATCTGATCGAGTCGCCGGAGACCGCGCTGTTGGATCTGTAG

Upstream 100 bases:

>100_bases
GCGGTTTTGACACACCCTACCCGCCTGCGCGGTTGGAAAAATTGTGGTTACCCGGCCCCGACCGGCTGTTGGACTGCGTC
GAACGTGTGCTGCGTCAGCC

Downstream 100 bases:

>100_bases
CCGGGCGGGCTGGCAGCTTTGGTTAGAGGATGGTCAGCAGCTCCACTGGCGTCTTGGGGCGTTGTTGGGCGAGGGTGAAG
ACACCGGCGTCGAAGGTGAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSGEDSIRSFPVPDLGEGLQEVTVTCWSVAVGDDVEINQTLCSVETAKAEVEIPSPYAGRIVELGGAEGDVLKVGAELVR
IDTGPTAVAQPNGEGAVPTLVGYGADAAIETSRRTSRPLAAPVVRKLAKELAVDLAALQRGSGAGGVITRADVLAAARGG
VGAGPDVRPVHGVHARMAEKMTLSHKEIPTAKASVEVICAELLRLRDRFVSAAPEITPFALTLRLLVIALKHNVILNSTW
VDSGEGPQVHVHRGVHLGFGAATERGLLVPVVTDAQDKNTRELASRVAELITGAREGTLTPAELRGSTFTVSNFGALGVD
DGVPVINHPEAAILGLGAIKPRPVVVGGEVVARPTMTLTCVFDHRVVDGAQVAQFMCELRDLIESPETALLDL

Sequences:

>Translated_393_residues
MSGEDSIRSFPVPDLGEGLQEVTVTCWSVAVGDDVEINQTLCSVETAKAEVEIPSPYAGRIVELGGAEGDVLKVGAELVR
IDTGPTAVAQPNGEGAVPTLVGYGADAAIETSRRTSRPLAAPVVRKLAKELAVDLAALQRGSGAGGVITRADVLAAARGG
VGAGPDVRPVHGVHARMAEKMTLSHKEIPTAKASVEVICAELLRLRDRFVSAAPEITPFALTLRLLVIALKHNVILNSTW
VDSGEGPQVHVHRGVHLGFGAATERGLLVPVVTDAQDKNTRELASRVAELITGAREGTLTPAELRGSTFTVSNFGALGVD
DGVPVINHPEAAILGLGAIKPRPVVVGGEVVARPTMTLTCVFDHRVVDGAQVAQFMCELRDLIESPETALLDL
>Mature_392_residues
SGEDSIRSFPVPDLGEGLQEVTVTCWSVAVGDDVEINQTLCSVETAKAEVEIPSPYAGRIVELGGAEGDVLKVGAELVRI
DTGPTAVAQPNGEGAVPTLVGYGADAAIETSRRTSRPLAAPVVRKLAKELAVDLAALQRGSGAGGVITRADVLAAARGGV
GAGPDVRPVHGVHARMAEKMTLSHKEIPTAKASVEVICAELLRLRDRFVSAAPEITPFALTLRLLVIALKHNVILNSTWV
DSGEGPQVHVHRGVHLGFGAATERGLLVPVVTDAQDKNTRELASRVAELITGAREGTLTPAELRGSTFTVSNFGALGVDD
GVPVINHPEAAILGLGAIKPRPVVVGGEVVARPTMTLTCVFDHRVVDGAQVAQFMCELRDLIESPETALLDL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=30.5882352941176, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI31711992, Length=302, Percent_Identity=30.4635761589404, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI19923748, Length=242, Percent_Identity=28.9256198347107, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI203098816, Length=225, Percent_Identity=29.3333333333333, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI203098753, Length=223, Percent_Identity=29.1479820627803, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=33.974358974359, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=28.1173594132029, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786305, Length=416, Percent_Identity=29.5673076923077, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=26.6821345707657, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=27.2511848341232, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=25.679012345679, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538894, Length=239, Percent_Identity=25.9414225941423, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=396, Percent_Identity=28.2828282828283, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=22.8888888888889, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=28.8018433179723, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24645909, Length=142, Percent_Identity=35.9154929577465, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 41031; Mature: 40900

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEDSIRSFPVPDLGEGLQEVTVTCWSVAVGDDVEINQTLCSVETAKAEVEIPSPYAGR
CCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHEECCCEEEECCCCCCCC
IVELGGAEGDVLKVGAELVRIDTGPTAVAQPNGEGAVPTLVGYGADAAIETSRRTSRPLA
EEEECCCCCCCEEECCEEEEECCCCCEEECCCCCCCCCEEEECCCCHHEECCHHCCCCHH
APVVRKLAKELAVDLAALQRGSGAGGVITRADVLAAARGGVGAGPDVRPVHGVHARMAEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
MTLSHKEIPTAKASVEVICAELLRLRDRFVSAAPEITPFALTLRLLVIALKHNVILNSTW
HHHCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEE
VDSGEGPQVHVHRGVHLGFGAATERGLLVPVVTDAQDKNTRELASRVAELITGAREGTLT
CCCCCCCEEEEECCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
PAELRGSTFTVSNFGALGVDDGVPVINHPEAAILGLGAIKPRPVVVGGEVVARPTMTLTC
HHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCEEECCCEEECCCEEEEE
VFDHRVVDGAQVAQFMCELRDLIESPETALLDL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SGEDSIRSFPVPDLGEGLQEVTVTCWSVAVGDDVEINQTLCSVETAKAEVEIPSPYAGR
CCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHEECCCEEEECCCCCCCC
IVELGGAEGDVLKVGAELVRIDTGPTAVAQPNGEGAVPTLVGYGADAAIETSRRTSRPLA
EEEECCCCCCCEEECCEEEEECCCCCEEECCCCCCCCCEEEECCCCHHEECCHHCCCCHH
APVVRKLAKELAVDLAALQRGSGAGGVITRADVLAAARGGVGAGPDVRPVHGVHARMAEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
MTLSHKEIPTAKASVEVICAELLRLRDRFVSAAPEITPFALTLRLLVIALKHNVILNSTW
HHHCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEE
VDSGEGPQVHVHRGVHLGFGAATERGLLVPVVTDAQDKNTRELASRVAELITGAREGTLT
CCCCCCCEEEEECCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
PAELRGSTFTVSNFGALGVDDGVPVINHPEAAILGLGAIKPRPVVVGGEVVARPTMTLTC
HHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCEEECCCEEECCCEEEEE
VFDHRVVDGAQVAQFMCELRDLIESPETALLDL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]