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The map label for this gene is 148823680

Identifier: 148823680

GI number: 148823680

Start: 2804335

End: 2805816

Strand: Reverse

Name: 148823680

Synonym: TBFG_12505

Alternate gene names: NA

Gene position: 2805816-2804335 (Counterclockwise)

Preceding gene: 148823681

Following gene: 148823679

Centisome position: 63.42

GC content: 66.26

Gene sequence:

>1482_bases
GTGGCCATGGCTGAGTCCGGCGAGTCCCCCAGGTTGTCCGACGAGCTTGGGCCGGTCGACTATTTGATGCATCGGGGCGA
GGCAAATCCGCGGACTCGTTCGGGGATCATGGCGCTGGAACTGCTGGACGGCACGCCGGACTGGGATCGCTTCCGGACCC
GATTCGAAAACGCCTCGCGACGGGTGTTGCGGCTGCGGCAGAAGGTGGTGGTGCCGACCTTGCCGACCGCGGCCCCGCGC
TGGGTGGTGGATCCGGACTTCAATCTGGACTTCCATGTGCGTCGGGTGCGTGTGTCCGGACCCGCCACGTTGCGCGAAGT
GCTCGATCTCGCCGAGGTGATTCTGCAGTCGCCGCTGGACATATCGCGCCCGCTGTGGACGGCCACCCTGGTGGAGGGTA
TGGCTGACGGCAGAGCGGCGATGCTGCTGCACGTCAGCCATGCGGTCACCGACGGTGTCGGCGGCGTGGAGATGTTCGCG
CAGATCTATGACCTCGAGCGCGATCCACCACCTAGGTCGACGCCGCCGCAACCCATTCCGGAGGATCTGTCCCCCAATGA
CCTGATGCGCCGGGGCATCAACCACCTGCCAATTGCTGTCGTCGGCGGTGTCCTGGACGCGCTGTCCGGGGCGGTATCGA
TGGCCGGGCGGGCTGTTCTGGAACCGGTGTCCACCGTGTCGGGGATCTTGGGCTACGCCAGGTCGGGCATCCGGGTGCTG
AACCGAGCTGCTGAACCCTCACCCCTACTGCGCCGGCGCAGCCTGACCACCCGTACCGAGGCCATCGACATCCGGCTCGC
CGACCTGCACAAGGCCGCAAAGGCCGGCGGCGGGTCGATCAACGACGCCTACCTTGCGGGTCTGTGTGGCGCGCTGCGCC
GCTACCACGAGGCCCTGGGCGTGCCGATCAGCACGCTGCCGATGGCGGTGCCGGTCAACCTGCGGGCCGAGGGCGATGCG
GCCGGCGGCAACCAGTTCACCGGTGTCAACCTGGCGGCGCCGGTGGGCACTATCGATCCGGTGGCTCGGATGAAGAAGAT
CCGGGCGCAGATGACCCAGCGTCGCGACGAGCCCGCGATGAACATCATCGGTTCCATTGCACCGGTGCTGAGTGTCTTGC
CGACGGCCGTGCTGGAGGGGATCACCGGTTCGGTGATCGGTTCCGATGTGCAAGCCAGCAATGTTCCGGTCTACCCAGGG
GATACCTACCTTGCCGGCGCGAAGATACTGCGGCAGTACGGTATTGGACCGTTGCCCGGTGTGGCGATGATGGTAGTGCT
AATTTCCCGGGGCGGGTGGTGCACCGTCACGGTGCGCTACGACAGGGCGTCGGTGCGAAACGACGAGTTGTTCGCTCAAT
GCCTGCAGGCGGGTTTCGACGAAATCCTTGCGCTGGCCGGTGGTCCGGCACCGCGTGTGCTGCCGGCGTCGTTCGATACT
CAAGGAGCCGGTTCAGTGCCTCGATCGGTGTCGGGCTCATGA

Upstream 100 bases:

>100_bases
GTGTTGGCACTGTGGTGCGTGGCGGTGGCGGGTTTGAAGCAGGAGGTCTGATCGGCGGTGACAGAGTTTGTGCGTCGAGT
TGCCCAACCCGCGCGGGGTC

Downstream 100 bases:

>100_bases
GCGCCGCTGACGAGCAGGGGGAAGAGCGGGCGACGCGGAAGTCGGCGCCGGATTTGCGCCTACCCGGTTCGGTAGCCGAG
ATTCTGGCCAGTCCTGCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv2484c/MT2557 [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAMAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPR
WVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFA
QIYDLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVL
NRAAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDA
AGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPG
DTYLAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDT
QGAGSVPRSVSGS

Sequences:

>Translated_493_residues
MAMAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPR
WVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFA
QIYDLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVL
NRAAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDA
AGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPG
DTYLAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDT
QGAGSVPRSVSGS
>Mature_492_residues
AMAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPRW
VVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQ
IYDLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLN
RAAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDAA
GGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGD
TYLAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQ
GAGSVPRSVSGS

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 52512; Mature: 52381

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASR
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHH
RVLRLRQKVVVPTLPTAAPRWVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLD
HHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
ISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQIYDLERDPPPRSTPPQPIP
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVL
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRAAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALG
HHCCCCCHHHHHHHCCHHHHHEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
VPISTLPMAVPVNLRAEGDAAGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAM
CCHHHCCEEECEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHH
NIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDTYLAGAKILRQYGIGPLPG
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
VAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDT
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
QGAGSVPRSVSGS
CCCCCCCCCCCCC
>Mature Secondary Structure 
AMAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASR
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHH
RVLRLRQKVVVPTLPTAAPRWVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLD
HHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
ISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQIYDLERDPPPRSTPPQPIP
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVL
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRAAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALG
HHCCCCCHHHHHHHCCHHHHHEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
VPISTLPMAVPVNLRAEGDAAGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAM
CCHHHCCEEECEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHH
NIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDTYLAGAKILRQYGIGPLPG
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
VAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDT
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
QGAGSVPRSVSGS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]