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The map label for this gene is estB [H]

Identifier: 148823663

GI number: 148823663

Start: 2780329

End: 2781513

Strand: Direct

Name: estB [H]

Synonym: TBFG_12488

Alternate gene names: 148823663

Gene position: 2780329-2781513 (Clockwise)

Preceding gene: 148823659

Following gene: 148823667

Centisome position: 62.84

GC content: 65.99

Gene sequence:

>1185_bases
ATGAATCAGCCTGACATCAAGGGCTCGTGCGCGTCGGAGTTCACCAAGGTACGCGACGCGTTCGAGCGCAACTTTGTGCT
GCGCAACGAGGTCGGCGCGGCCGTCGCGGTGTGGGTCGACGGGGATCTTGTCGTCAACCTGTGGGGCGGCTCCGCCGACG
CCGGCGGTACCCGGCCCTGGCAGCACGACACGCTGGCCACCGTGCTGTCCGGTACCAAGGCACTAACGGCCACGTGTGTG
CATCAGCTCGTCGATCGCGGTGAGCTTGACCTGCATGCGCCGGTGGCACGCTACTGGCCCGAGTTCGGACAGGCGGGTAA
GCAGGCCATCACGCTGGCGATGGTGATGAGCCACCGCTCCGGGGCGATCGGGCCGCGCGGACGGCTGGGCTGGGAGCAGG
TCGCCGATTGGGATTTTGTCTGCGAGCAACTGGCCGCCGCCGAACCGTGGTGGCAGCCGGGTGCCGCGCAGGGCTACCAC
ATGACCACCTTCGGTTTCATCCTCGGCGAAGTGTTCCGCCGCGTCACAGGCCGTACGGTCGGTCAATACCTGCGTACCGA
GATCGCTGAGCCGCTGGGTGCGGACGTCCACATTGGCTTGCATCCCGGCGAACAGCTCCGCTGCGCCGATCTAGTTGATA
AGCCGCACATCCGCCAATTGCTGGCCGACGTCCAAGCCCCCGGCTACCCCACCAGCCTAAACGAACATCCCAAGGCTGCA
TTGTCGGTGTCGATGGGCTTCGCCCCCGACGACGAACTCGGCTCCAACGACCTGCAGCTGTGGCGTCAGATCGAATTCCC
CGGCACCAACGGCCAGGTGTCTGCGCTGGGGCTGGCGACGTTCTACAACGGGCTTGCCCAGGAGAAGCTGCTCAGCCGCG
AGCACATGGAGCTGGTCCGGGTCTCACAGGGCGGCTTCGACACCGATCTGGTGCTCGGCCCGAGGGTCGCCGACCATGGC
TGGGGTCTGGGCTACATGCTCAACCAGCGCGGCGTCAATGGACCCAACCCACGGATTTTCGGGCATGGTGGCCTCGGCGG
CTCGTTTGGGTTCGTCGACCTCGAGCACCGGATCGGCTACGCCTACGTGATGAACCGCTTCGACGCCACCAAGGCCAACG
CGGATCCGCGCAGCGTCGTCCTGTCCAACGAGGTCTACGCCGCGCTCGGGGTAAACCGTTCCTAG

Upstream 100 bases:

>100_bases
ATGGTAGAGCCCTAGTCTTCCAAACTAGCGACGCGGGTTCGATTCCCGTCGCCCGCTCGGGCCATGCGTTTGTTCGGCAG
AAAGGCGCCATGCGCGACCC

Downstream 100 bases:

>100_bases
ACGGCTAGCCACCAGGCGGTCAGGTCTGACAGACCGGGCACCAGAAAACATTGCGGCCCTCGAGCAGTGCCGTGCGGATC
ACTCCCCCACACACCCGACA

Product: esterase/lipase lipP

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNQPDIKGSCASEFTKVRDAFERNFVLRNEVGAAVAVWVDGDLVVNLWGGSADAGGTRPWQHDTLATVLSGTKALTATCV
HQLVDRGELDLHAPVARYWPEFGQAGKQAITLAMVMSHRSGAIGPRGRLGWEQVADWDFVCEQLAAAEPWWQPGAAQGYH
MTTFGFILGEVFRRVTGRTVGQYLRTEIAEPLGADVHIGLHPGEQLRCADLVDKPHIRQLLADVQAPGYPTSLNEHPKAA
LSVSMGFAPDDELGSNDLQLWRQIEFPGTNGQVSALGLATFYNGLAQEKLLSREHMELVRVSQGGFDTDLVLGPRVADHG
WGLGYMLNQRGVNGPNPRIFGHGGLGGSFGFVDLEHRIGYAYVMNRFDATKANADPRSVVLSNEVYAALGVNRS

Sequences:

>Translated_394_residues
MNQPDIKGSCASEFTKVRDAFERNFVLRNEVGAAVAVWVDGDLVVNLWGGSADAGGTRPWQHDTLATVLSGTKALTATCV
HQLVDRGELDLHAPVARYWPEFGQAGKQAITLAMVMSHRSGAIGPRGRLGWEQVADWDFVCEQLAAAEPWWQPGAAQGYH
MTTFGFILGEVFRRVTGRTVGQYLRTEIAEPLGADVHIGLHPGEQLRCADLVDKPHIRQLLADVQAPGYPTSLNEHPKAA
LSVSMGFAPDDELGSNDLQLWRQIEFPGTNGQVSALGLATFYNGLAQEKLLSREHMELVRVSQGGFDTDLVLGPRVADHG
WGLGYMLNQRGVNGPNPRIFGHGGLGGSFGFVDLEHRIGYAYVMNRFDATKANADPRSVVLSNEVYAALGVNRS
>Mature_394_residues
MNQPDIKGSCASEFTKVRDAFERNFVLRNEVGAAVAVWVDGDLVVNLWGGSADAGGTRPWQHDTLATVLSGTKALTATCV
HQLVDRGELDLHAPVARYWPEFGQAGKQAITLAMVMSHRSGAIGPRGRLGWEQVADWDFVCEQLAAAEPWWQPGAAQGYH
MTTFGFILGEVFRRVTGRTVGQYLRTEIAEPLGADVHIGLHPGEQLRCADLVDKPHIRQLLADVQAPGYPTSLNEHPKAA
LSVSMGFAPDDELGSNDLQLWRQIEFPGTNGQVSALGLATFYNGLAQEKLLSREHMELVRVSQGGFDTDLVLGPRVADHG
WGLGYMLNQRGVNGPNPRIFGHGGLGGSFGFVDLEHRIGYAYVMNRFDATKANADPRSVVLSNEVYAALGVNRS

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983659, Length=376, Percent_Identity=33.7765957446808, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17538001, Length=378, Percent_Identity=32.8042328042328, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71983666, Length=299, Percent_Identity=35.7859531772575, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17535191, Length=375, Percent_Identity=31.2, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17538474, Length=411, Percent_Identity=29.4403892944039, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17535177, Length=374, Percent_Identity=28.6096256684492, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71990590, Length=381, Percent_Identity=27.5590551181102, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 42840; Mature: 42840

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPDIKGSCASEFTKVRDAFERNFVLRNEVGAAVAVWVDGDLVVNLWGGSADAGGTRPW
CCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCC
QHDTLATVLSGTKALTATCVHQLVDRGELDLHAPVARYWPEFGQAGKQAITLAMVMSHRS
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCC
GAIGPRGRLGWEQVADWDFVCEQLAAAEPWWQPGAAQGYHMTTFGFILGEVFRRVTGRTV
CCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GQYLRTEIAEPLGADVHIGLHPGEQLRCADLVDKPHIRQLLADVQAPGYPTSLNEHPKAA
HHHHHHHHHHHCCCCEEEECCCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
LSVSMGFAPDDELGSNDLQLWRQIEFPGTNGQVSALGLATFYNGLAQEKLLSREHMELVR
EEEEECCCCCCCCCCCHHHHHEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VSQGGFDTDLVLGPRVADHGWGLGYMLNQRGVNGPNPRIFGHGGLGGSFGFVDLEHRIGY
HCCCCCCCEEEECCEECCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEHHHHCCH
AYVMNRFDATKANADPRSVVLSNEVYAALGVNRS
HHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCC
>Mature Secondary Structure
MNQPDIKGSCASEFTKVRDAFERNFVLRNEVGAAVAVWVDGDLVVNLWGGSADAGGTRPW
CCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCC
QHDTLATVLSGTKALTATCVHQLVDRGELDLHAPVARYWPEFGQAGKQAITLAMVMSHRS
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCC
GAIGPRGRLGWEQVADWDFVCEQLAAAEPWWQPGAAQGYHMTTFGFILGEVFRRVTGRTV
CCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GQYLRTEIAEPLGADVHIGLHPGEQLRCADLVDKPHIRQLLADVQAPGYPTSLNEHPKAA
HHHHHHHHHHHCCCCEEEECCCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
LSVSMGFAPDDELGSNDLQLWRQIEFPGTNGQVSALGLATFYNGLAQEKLLSREHMELVR
EEEEECCCCCCCCCCCHHHHHEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VSQGGFDTDLVLGPRVADHGWGLGYMLNQRGVNGPNPRIFGHGGLGGSFGFVDLEHRIGY
HCCCCCCCEEEECCEECCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEHHHHCCH
AYVMNRFDATKANADPRSVVLSNEVYAALGVNRS
HHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]