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The map label for this gene is sbpA [H]

Identifier: 148823603

GI number: 148823603

Start: 2711297

End: 2712367

Strand: Reverse

Name: sbpA [H]

Synonym: TBFG_12428

Alternate gene names: 148823603

Gene position: 2712367-2711297 (Counterclockwise)

Preceding gene: 148823604

Following gene: 148823602

Centisome position: 61.3

GC content: 62.93

Gene sequence:

>1071_bases
ATGCTCTCCTTGACGCTTTCTGAAGCGAGCTGCATCGCTAGCGCATCCCGCTGGCGGCACATTATCCCTGCCGGGGTGGT
GTGCGCATTGATCGCCGGTATCGGCGTGGGGTGTCATGGCGGTCCCAGCGACGTGGTCGGCCGTGCGGGACCGGACCGTG
CGCATACGAGCATCACCCTGGTCGCCTACGCCGTCCCGGAACCCGGCTGGAGTGCGGTGATTCCCGCGTTCAACGCTTCC
GAACAGGGCCGGGGAGTCCAGGTGATTACCTCATATGGCGCGTCGGCCGACCAGTCGCGCGGTGTTGCCGACGGTAAACC
GGCCGACCTGGTGAACTTCTCGGTCGAACCGGACATCGCTCGCCTGGTCAAGGCCGGCAAGGTTGACAAGGACTGGGACG
CCGATGCCACCAAGGGCATCCCGTTCGGGTCGGTGGTGACGTTTGTGGTCCGCGCGGGTAACCCGAAGAACATCAGAGAT
TGGGATGACCTGTTGCGCCCGGGTATTGAGGTCATCACGCCCAGTCCGCTGAGTTCGGGTTCTGCCAAGTGGAATCTGCT
AGCCCCCTACGCCGCGAAAAGTGACGGTGGCCGGAATAACCAAGCGGGGATCGACTTTGTCAATACATTGGTGAATGAAC
ACGTCAAATTGCGCCCCGGGTCGGGGCGGGAAGCCACCGATGTTTTTGTCCAGGGCAGCGGTGACGTGTTGATCAGCTAC
GAGAACGAAGCCATCGCCACCGAGCGGGCGGGCAAACCGGTGCAGCACGTCACCCCGCCGCAGACGTTCAAGATCGAAAA
TCCGTTGGCCGTAGTGGCGACCAGCACACACCTTGGAGCGGCGACCGCATTCAGAAACTTCCAGTACACCGTGCAGGCGC
AGAAGTTATGGGCGCAGGCCGGTTTCCGGCCGGTCGATCCGGCGGTCGCCGCCGATTTTGCCGACCTGTTTCCGGTGCCG
GCGAAACTGTGGACGATCGCCGACCTCGGTGGCTGGGGCAGCGTGGATCCTCAGCTGTTCGACAAGGCGACCGGCAGCAT
CACCAAGATTTATCTGCGGGCCACCGGATGA

Upstream 100 bases:

>100_bases
GCGCGGTCTGATGTGGAGTTCGTGAGTCCTGCAATGTTCAGCGATCGCCTTTCCGCCGCTACGGGAACGTTGTCCAAAGT
CCCGCACCGGAAGGTCGCCA

Downstream 100 bases:

>100_bases
CGGAATCGTTGGTCGGCGAACGCCGCGCGCCGCAGTTCCGCGCGCGCCTTTCCGGCCCCGCGGGCCCCCCTTCCGTTCGG
GTCGGTATGGCAGTGGTGTG

Product: lipoprotein (sulfate-binding) subI

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITLVAYAVPEPGWSAVIPAFNAS
EQGRGVQVITSYGASADQSRGVADGKPADLVNFSVEPDIARLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRD
WDDLLRPGIEVITPSPLSSGSAKWNLLAPYAAKSDGGRNNQAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISY
ENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQAGFRPVDPAVAADFADLFPVP
AKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG

Sequences:

>Translated_356_residues
MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITLVAYAVPEPGWSAVIPAFNAS
EQGRGVQVITSYGASADQSRGVADGKPADLVNFSVEPDIARLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRD
WDDLLRPGIEVITPSPLSSGSAKWNLLAPYAAKSDGGRNNQAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISY
ENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQAGFRPVDPAVAADFADLFPVP
AKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG
>Mature_356_residues
MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITLVAYAVPEPGWSAVIPAFNAS
EQGRGVQVITSYGASADQSRGVADGKPADLVNFSVEPDIARLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRD
WDDLLRPGIEVITPSPLSSGSAKWNLLAPYAAKSDGGRNNQAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISY
ENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQAGFRPVDPAVAADFADLFPVP
AKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=278, Percent_Identity=35.2517985611511, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788765, Length=296, Percent_Identity=31.0810810810811, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR006311
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37419; Mature: 37419

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITL
CEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEE
VAYAVPEPGWSAVIPAFNASEQGRGVQVITSYGASADQSRGVADGKPADLVNFSVEPDIA
EEEECCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHH
RLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRDWDDLLRPGIEVITPSPLSSG
HHHHHCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCC
SAKWNLLAPYAAKSDGGRNNQAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISY
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCEEEEE
ENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQA
CCCEEEHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHCCEEEEHHHHHHHHC
GFRPVDPAVAADFADLFPVPAKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG
CCCCCCHHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure
MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITL
CEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEE
VAYAVPEPGWSAVIPAFNASEQGRGVQVITSYGASADQSRGVADGKPADLVNFSVEPDIA
EEEECCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHH
RLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRDWDDLLRPGIEVITPSPLSSG
HHHHHCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCC
SAKWNLLAPYAAKSDGGRNNQAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISY
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCEEEEE
ENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQA
CCCEEEHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHCCEEEEHHHHHHHHC
GFRPVDPAVAADFADLFPVPAKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG
CCCCCCHHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]