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The map label for this gene is dnaG

Identifier: 148823544

GI number: 148823544

Start: 2631330

End: 2633249

Strand: Reverse

Name: dnaG

Synonym: TBFG_12367

Alternate gene names: 148823544

Gene position: 2633249-2631330 (Counterclockwise)

Preceding gene: 148823545

Following gene: 148823541

Centisome position: 59.52

GC content: 66.88

Gene sequence:

>1920_bases
ATGTCCGGCCGGATCTCCGATCGCGATATCGCGGCAATCCGCGAGGGGGCCCGTATCGAGGATGTCGTCGGCGACTATGT
CCAGCTCAGGCGTGCCGGTGCCGATTCGCTGAAGGGGCTGTGCCCGTTTCACAACGAGAAGTCCCCGTCCTTTCACGTGC
GGCCCAACCACGGCCACTTCCACTGCTTCGGCTGCGGCGAGGGCGGCGACGTGTATGCGTTCATCCAGAAGATCGAGCAC
GTCAGCTTCGTCGAGGCGGTTGAGTTGCTTGCCGACCGGATCGGCCACACGATCAGCTACACCGGTGCGGCCACCAGCGT
GCAGCGCGACCGCGGCAGTCGCAGCAGGCTGCTGGCCGCCAACGCGGCCGCGGCAGCGTTCTATGCGCAGGCGCTGCAGT
CCGACGAGGCGGCACCGGCTCGCCAGTACCTGACCGAACGCAGCTTCGACGCGGCGGCCGCTCGTAAATTCGGCTGTGGG
TTCGCCCCGTCGGGCTGGGACTCATTGACAAAACATCTGCAGCGCAAGGGTTTTGAGTTCGAGGAACTGGAGGCCGCGGG
ACTGTCCCGGCAGGGCCGGCACGGCCCGATGGACCGGTTTCACCGTCGGCTGCTGTGGCCCATCCGCACCTCGGCCGGCG
AGGTGGTCGGGTTCGGTGCCCGGCGACTGTTCGACGACGACGCGATGGAGGCCAAGTACGTCAACACGCCCGAGACATTG
CTGTACAAGAAGTCATCGGTGATGTTCGGCATCGACTTGGCCAAACGCGACATCGCCAAGGGACATCAGGCCGTCGTCGT
CGAGGGCTACACCGATGTCATGGCCATGCATCTGGCCGGGGTCACCACCGCGGTGGCGTCGTGCGGGACCGCATTCGGCG
GCGAGCACCTGGCGATGCTGCGCAGACTGATGATGGACGACAGCTTCTTCCGCGGCGAGCTGATCTACGTTTTCGACGGC
GACGAGGCCGGCCGCGCCGCCGCGCTCAAGGCCTTCGACGGTGAGCAGAAGCTGGCTGGTCAGTCTTTCGTCGCGGTGGC
TCCCGACGGCATGGACCCCTGCGACCTACGACTGAAGTGCGGCGACGCTGCCCTGCGCGACCTCGTGGCACGACGAACCC
CATTGTTCGAGTTCGCGATTCGCGCCGCGATTGCCGAGATGGACCTGGATAGCGCCGAGGGCAGGGTGGCCGCGCTGCGC
CGTTGCGTACCGATGGTGGGCCAGATCAAAGACCCCACGCTGCGTGACGAATACGCCCGTCAGCTCGCCGGCTGGGTCGG
GTGGGCCGACGTCGCACAGGTCATCGGTCGGGTGCGGGGTGAGGCCAAACGAACCAAGCACCCCAGGCTGGGCAGACTGG
GATCGACGACCATCGCCCGGGCAGCGCAGCGGCCTACCGCCGGCCCCCCGACCGAACTCGCAGTCCGACCTGATCCGCGT
GACCCGACCCTGTGGCCACAGCGTGAGGCACTCAAATCGGCGTTGCAGTACCCGGCGTTGGCCGGCCCGGTGTTCGACGC
GCTGACCGTCGAAGGCTTCACGCACCCCGAGTACGCGGCTGTGCGCGCGGCCATCGACACGGCGGGCGGTACCTCGGCTG
GCCTTAGCGGTGCGCAGTGGCTGGATATGGTGCGCCAGCAGACGACATCAACCGTGACCTCGGCTTTGATCAGCGAGCTG
GGGGTGGAGGCGATTCAGGTCGACGACGACAAGCTGCCGCGCTATATCGCCGGCGTGCTGGCCCGTCTGCAGGAGGTTTG
GTTGGGGCGGCAGATCGCCGAGGTGAAGTCCAAACTGCAGCGCATGTCGCCGATCGAGCAAGGCGACGAATACCACGCGC
TGTTCGGCGACCTGGTCGCCATGGAGGCCTATCGGCGCAGCCTGTTAGAGCAGGCCAGCGGCGACGATCTCACCGCGTGA

Upstream 100 bases:

>100_bases
CGGGTCGTCGTTGATCAGATCGCCTCCTATACCGAGGGTCGGTTGGAACGCATCGATGCCGATCAGCTTGGCGTCAGCAG
GAACGCTTTAGACTGAGCCG

Downstream 100 bases:

>100_bases
GGGTCAGCGCGCGGTTTTCGGCTGCCGCTCGACCCCGCGCTGGACAACGGCGGTCTGGTTGTCGATCTCGGCCAGGGTCA
CAAACCCAGCATCGGGTGAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCGEGGDVYAFIQKIEH
VSFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAANAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCG
FAPSGWDSLTKHLQRKGFEFEELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETL
LYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAMLRRLMMDDSFFRGELIYVFDG
DEAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKCGDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALR
RCVPMVGQIKDPTLRDEYARQLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPR
DPTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQWLDMVRQQTTSTVTSALISEL
GVEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA

Sequences:

>Translated_639_residues
MSGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCGEGGDVYAFIQKIEH
VSFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAANAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCG
FAPSGWDSLTKHLQRKGFEFEELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETL
LYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAMLRRLMMDDSFFRGELIYVFDG
DEAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKCGDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALR
RCVPMVGQIKDPTLRDEYARQLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPR
DPTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQWLDMVRQQTTSTVTSALISEL
GVEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
>Mature_638_residues
SGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCGEGGDVYAFIQKIEHV
SFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAANAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCGF
APSGWDSLTKHLQRKGFEFEELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETLL
YKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAMLRRLMMDDSFFRGELIYVFDGD
EAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKCGDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALRR
CVPMVGQIKDPTLRDEYARQLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPRD
PTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQWLDMVRQQTTSTVTSALISELG
VEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=515, Percent_Identity=32.4271844660194, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_MYCBO (P63963)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856021.1
- ProteinModelPortal:   P63963
- SMR:   P63963
- EnsemblBacteria:   EBMYCT00000014300
- GeneID:   1094360
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2372c
- GeneTree:   EBGT00050000015601
- HOGENOM:   HBG573408
- OMA:   HDERSPS
- ProtClustDB:   PRK05667
- BioCyc:   MBOV233413:MB2372C-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 69594; Mature: 69462

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHF
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCGEGGDVYAFIQKIEHVSFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCCCHHEEHH
NAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCGFAPSGWDSLTKHLQRKGFEF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCH
EELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCCCCCCEECCCCHHH
LYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAML
HEECCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRLMMDDSFFRGELIYVFDGDEAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKC
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEECCCCCCCCCEEEEC
GDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALRRCVPMVGQIKDPTLRDEYAR
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
QLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPR
HHHHHCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCC
DPTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQW
CCCCCCHHHHHHHHHHCCCCCCHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHH
LDMVRQQTTSTVTSALISELGVEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQ
HHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHF
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCGEGGDVYAFIQKIEHVSFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCCCHHEEHH
NAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCGFAPSGWDSLTKHLQRKGFEF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCH
EELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCCCCCCEECCCCHHH
LYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAML
HEECCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRLMMDDSFFRGELIYVFDGDEAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKC
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEECCCCCCCCCEEEEC
GDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALRRCVPMVGQIKDPTLRDEYAR
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
QLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPR
HHHHHCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCC
DPTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQW
CCCCCCHHHHHHHHHHCCCCCCHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHH
LDMVRQQTTSTVTSALISELGVEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQ
HHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972