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The map label for this gene is stp

Identifier: 148823534

GI number: 148823534

Start: 2617505

End: 2619118

Strand: Reverse

Name: stp

Synonym: TBFG_12357

Alternate gene names: 148823534

Gene position: 2619118-2617505 (Counterclockwise)

Preceding gene: 148823538

Following gene: 148823530

Centisome position: 59.2

GC content: 65.24

Gene sequence:

>1614_bases
ATGAACCGCACGCAGCTCCTCACGCTCATCGCCACTGGCCTGGGCCTGTTCATGATCTTTCTCGACGCACTGATCGTGAA
CGTGGCGCTGCCCGACATCCAGCGCAGTTTCGCGGTGGGGGAGGACGGCCTGCAGTGGGTGGTGGCGTCCTACAGCCTCG
GAATGGCGGTCTTCATCATGTCGGCGGCGACGCTTGCCGACCTCGACGGTCGGCGCCGCTGGTACTTGATAGGTGTTTCG
TTGTTCACGCTGGGATCTATCGCCTGCGGGTTGGCGCCCTCAATCGCGGTGCTCACCACCGCGCGGGGCGCGCAGGGCCT
AGGTGCGGCGGCCGTCAGCGTAACCTCGCTCGCCCTGGTCAGCGCGGCCTTTCCGGAGGCGAAGGAGAAGGCGCGCGCCA
TCGGGATCTGGACCGCCATCGCGAGCATCGGTACGACGACGGGCCCGACACTGGGCGGTCTGCTGGTGGACCAGTGGGGG
TGGCGCAGCATCTTCTATGTCAACCTGCCCATGGGCGCACTCGTCCTCTTCCTGACTTTGTGCTACGTCGAGGAGTCGTG
TAACGAGCGGGCACGCCGCTTCGATCTGTCCGGGCAGCTGCTGTTCATCGTTGCCGTGGGTGCCCTGGTGTATGCGGTCA
TCGAAGGCCCCCAGATCGGCTGGACGTCTGTCCAGACCATCGTGATGCTCTGGACAGCCGCCGTCGGCTGCGCCCTGTTC
GTCTGGCTTGAGCGCAGATCATCGAATCCGATGATGGATCTGACCCTGTTCCGCGACACCTCATACGCCCTGGCCATCGC
GACCATCTGCACGGTGTTCTTCGCCGTCTATGGGATGCTGCTGCTCACCACCCAGTTCCTGCAGAACGTGCGCGGCTATA
CCCCGAGCGTGACCGGCCTGATGATCCTTCCGTTCAGTGCGGCCGTGGCCATCGTGTCGCCACTTGTTGGGCACCTGGTT
GGCCGGATCGGAGCACGCGTTCCCATCCTGGCGGGGCTGTGCATGTTGATGCTGGGTCTGCTCATGCTCATCTTCAGCGA
GCACCGGAGCTCGGCCCTGGTCCTGGTCGGGCTGGGCTTGTGCGGGAGCGGCGTCGCGTTGTGCCTGACGCCGATCACGA
CCGTCGCCATGACCGCGGTCCCCGCCGAGCGTGCGGGCATGGCGTCCGGCATCATGAGTGCTCAGCGGGCGATCGGCTCG
ACGATCGGGTTCGCGGTTTTGGGCTCCGTCCTTGCCGCCTGGCTTTCGGCCACACTCGAGCCCCATCTGGAGCGCGCGGT
GCCCGACCCGGTCCAGCGCCACGTCCTTGCCGAGATCATTATCGACAGTGCCAATCCGCGAGCGCATGTGGGCGGAATCG
TCCCCCGGCGGCACATTGAGCATCGCGACCCCGTCGCGATTGCGGAGGAGGATTTCATCGAAGGCATTCGGGTGGCGTTG
CTCGTCGCCACTGCAACACTGGCTGTCGTGTTTCTCGCCGGCTGGCGATGGTTTCCGCGTGACGTGCACACCGCGGGCAG
CGACCTAAGCGAGAGGCTGCCTACAGCGATGACCGTCGAGTGCGCGGTTAGTCATATGCCGGGAGCCACATGGTGTCGAC
TATGGCCTGCCTGA

Upstream 100 bases:

>100_bases
CCATTCGGCTCGAATCGACCGAAGCGGCTGACGCCGTCGCTGGCGCAGCCTAGTGTCTGGTCACAGATCGCCACCGAAAT
CCATGTAGATCGGGGCCCGC

Downstream 100 bases:

>100_bases
CGTCGTCGTGCGTCCTGGAAGCCACCCCGTCTTGGACGGCGGCCCGATAGACAGCTTCGGCGACCGTTGTCGAGATGGCC
CGCAGATTTTGGACATCCGG

Product: integral membrane transport protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLDGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA

Sequences:

>Translated_537_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLDGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA
>Mature_537_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLDGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA

Specific function: Contributes to spectinomycin and tetracycline resistance

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=28.3251231527094, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=28.641975308642, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788710, Length=401, Percent_Identity=29.6758104738155, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=29.5121951219512, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=28.4653465346535, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=29.559748427673, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87082231, Length=175, Percent_Identity=29.1428571428571, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=474, Percent_Identity=23.2067510548523, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=393, Percent_Identity=20.8651399491094, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=150, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=26.797385620915, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): STP_MYCTU (P71879)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   F70705
- RefSeq:   NP_216849.1
- RefSeq:   NP_336873.1
- ProteinModelPortal:   P71879
- EnsemblBacteria:   EBMYCT00000001385
- EnsemblBacteria:   EBMYCT00000072160
- GeneID:   887274
- GeneID:   924017
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2395
- KEGG:   mtu:Rv2333c
- TIGR:   MT2395
- TubercuList:   Rv2333c
- GeneTree:   EBGT00050000014781
- HOGENOM:   HBG679341
- OMA:   QAVTAHR
- ProtClustDB:   CLSK872052
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 57116; Mature: 57116

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x104e885c)-; HASH(0x18d11f74)-; HASH(0x1be40378)-; HASH(0x1be24ee4)-; HASH(0x1b9d6a88)-; HASH(0x1be1e85c)-; HASH(0x1be39710)-; HASH(0x1ad70980)-; HASH(0x1be4d094)-; HASH(0x19bab6b0)-; HASH(0x1be19678)-; HASH(0x1be73bcc)-; HASH(0x1be1e688)-; HASH(0x1a59a67c)-;

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAATLADLDGRRRWYLIGVSLFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALV
HHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHH
SAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHH
CYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGL
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
MILPFSAAVAIVSPLVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGL
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
CGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLAAWLSATLE
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCEECHHHHHHHHHHHH
LVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCEEHHHHHHCCCCCCCEEECCH
>Mature Secondary Structure
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAATLADLDGRRRWYLIGVSLFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALV
HHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHH
SAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHH
CYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGL
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
MILPFSAAVAIVSPLVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGL
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
CGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLAAWLSATLE
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCEECHHHHHHHHHHHH
LVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCEEHHHHHHCCCCCCCEEECCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036