Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 148823424

GI number: 148823424

Start: 2496730

End: 2497422

Strand: Direct

Name: lipB [H]

Synonym: TBFG_12245

Alternate gene names: 148823424

Gene position: 2496730-2497422 (Clockwise)

Preceding gene: 148823423

Following gene: 148823425

Centisome position: 56.43

GC content: 68.69

Gene sequence:

>693_bases
GTGACGGGTTCTATCCGGTCGAAGCTGTCCGCGATCGACGTCCGCCAGCTGGGGACCGTCGACTACCGGACCGCGTGGCA
GCTACAGCGAGAGCTAGCCGACGCCCGGGTCGCCGGCGGCGCCGACACGCTGCTGCTGTTGGAACACCCCGCGGTCTACA
CCGCCGGACGGCGTACCGAGACACACGAGCGACCCATTGACGGCACTCCGGTCGTCGACACCGACCGCGGCGGCAAGATC
ACCTGGCACGGTCCGGGGCAATTGGTCGGCTACCCGATCATCGGGCTGGCCGAACCCCTCGACGTGGTCAATTACGTTCG
GCGCCTTGAAGAATCGCTGATCCAAGTCTGCGCCGATCTGGGCCTGCACGCCGGCCGCGTCGACGGCCGGTCCGGGGTCT
GGCTGCCCGGCAGGCCGGCGCGCAAGGTCGCGGCCATCGGTGTCCGGGTGTCGCGGGCGACGACACTGCACGGGTTTGCG
CTCAACTGCGATTGTGATTTGGCTGCCTTCACCGCCATCGTGCCATGCGGAATCAGTGACGCCGCAGTGACATCGCTGTC
CGCCGAACTCGGCCGTACGGTCACCGTCGACGAGGTCCGCGCGACGGTCGCCGCCGCTGTCTGCGCCGCTCTGGACGGCG
TCCTACCGGTCGGTGACCGCGTGCCCTCACACGCCGTACCATCGCCGTTATGA

Upstream 100 bases:

>100_bases
AACACCATTGGCGAGGCGCTCGGCTACGCCACCACCCGGCCCGGCTAGGCTTGACCCCGTCTGCCCAGCCGTGCGCTGGC
GGCCGAGTAGCCTAGCTATC

Downstream 100 bases:

>100_bases
GTGTCGCTGCCGAGGGCCGGCGCCTGTTACGCCTGGAGGTGCGCAACGCGCAGACCCCAATCGAGCGCAAACCGCCGTGG
ATCAAGACACGAGCCCGCAT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVDTDRGGKI
TWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFA
LNCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL

Sequences:

>Translated_230_residues
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVDTDRGGKI
TWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFA
LNCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
>Mature_229_residues
TGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVDTDRGGKIT
WHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFAL
NCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=207, Percent_Identity=41.0628019323672, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI87081767, Length=178, Percent_Identity=34.8314606741573, Blast_Score=106, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355483, Length=224, Percent_Identity=35.2678571428571, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24211; Mature: 24080

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTE
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
THERPIDGTPVVDTDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADL
CCCCCCCCCCEEECCCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFALNCDCDLAAFTAIVPCGISD
CCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECEEEEEEEEEECCCCHHHHHHHHCCCCCH
AAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
HHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
THERPIDGTPVVDTDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADL
CCCCCCCCCCEEECCCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFALNCDCDLAAFTAIVPCGISD
CCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECEEEEEEEEEECCCCHHHHHHHHCCCCCH
AAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
HHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]