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The map label for this gene is dosT

Identifier: 148823243

GI number: 148823243

Start: 2287399

End: 2289120

Strand: Reverse

Name: dosT

Synonym: TBFG_12064

Alternate gene names: 148823243

Gene position: 2289120-2287399 (Counterclockwise)

Preceding gene: 148823244

Following gene: 148823242

Centisome position: 51.74

GC content: 65.39

Gene sequence:

>1722_bases
GTGACACACCCTGACAGGGCGAACGTTAACCCTGGCAGCCCGCCATTGCGCGAGACACTGTCGCAGCTTCGGTTGCGAGA
GCTGCTGCTGGAAGTCCAAGACCGGATTGAACAGATCGTCGAAGGCCGCGACCGGCTGGATGGTCTGATCGACGCCATCT
TGGCGATCACATCGGGACTCAAGCTCGACGCCACCCTGCGCGCCATTGTGCATACCGCCGCCGAGTTGGTGGATGCCCGC
TACGGGGCGCTCGGGGTGCGCGGTTACGACCATAGATTGGTCGAATTCGTCTACGAGGGGATCGACGAAGAGACCCGGCA
CCTCATCGGCTCATTGCCGGAGGGGCGAGGTGTTCTCGGCGCGCTGATCGAGGAGCCAAAGCCGATCCGGCTGGACGATA
TCTCGCGGCATCCCGCATCGGTTGGATTTCCGCTGCACCATCCGCCGATGCGGACCTTCCTCGGGGTTCCAGTGCGCATC
CGCGACGAGGTGTTCGGCAATCTTTACTTGACCGAGAAGGCAGATGGCCAGCCGTTCAGCGATGACGACGAGGTGCTGGT
GCAGGCGCTGGCCGCCGCGGCCGGGATTGCCGTTGACAATGCCCGTCTCTTCGAGGAATCACGGACCCGGGAAGCGTGGA
TCGAGGCAACCCGCGACATCGGAACGCAGATGCTGGCCGGTGCGGACCCGGCCATGGTGTTTCGGCTCATCGCCGAGGAA
GCGTTGACGTTGATGGCTGGGGCAGCCACCTTGGTGGCGGTGCCGCTCGACGACGAAGCGCCGGCTTGCGAGGTCGACGA
CCTGGTCATCGTAGAGGTGGCCGGAGAGATCTCCCCGGCGGTCAAGCAAATGACGGTTGCCGTCAGCGGAACGTCGATCG
GGGGAGTCTTTCACGACCGTACGCCCCGCCGGTTCGACCGGCTTGACCTCGCGGTCGACGGCCCGGTGGAGCCCGGGCCC
GCCCTGGTGCTGCCGCTGCGTGCCGCCGACACTGTTGCCGGTGTGCTGGTCGCACTTCGCAGTGCCGATGAACAGCCGTT
CAGCGACAAACAGCTCGATATGATGGCCGCCTTCGCTGACCAGGCTGCGCTCGCCTGGCGGCTGGCGACCGCGCAGCGAC
AGATGCGAGAAGTGGAGATCCTGACCGATCGCGACCGGATCGCACGTGATCTGCATGACCACGTCATCCAGCGGCTCTTC
GCAGTCGGGCTCACCCTGCAGGGTGCCGCTCCGCGAGCACGTGTCCCCGCCGTCCGGGAATCCATCTACAGCAGCATCGA
CGATCTCCAGGAAATCATTCAGGAGATTCGATCTGCGATCTTCGACCTACACGCCGGGCCCTCCCGGGCGACGGGTCTGC
GCCACCGACTGGACAAGGTCATCGACCAACTCGCGATCCCCGCGCTGCACACCACGGTCCAGTACACGGGCCCGCTGTCC
GTTGTCGACACCGTCCTGGCCAACCACGCCGAAGCGGTTCTGCGGGAGGCGGTTAGCAACGCTGTCCGGCACGCGAACGC
GACCAGCCTGGCCATCAACGTCAGCGTCGAGGATGATGTGCGGGTCGAGGTCGTCGACGACGGTGTCGGCATCTCCGGCG
ACATCACCGAAAGCGGCCTGCGCAATCTCCGCCAGCGTGCCGACGACGCGGGCGGCGAATTCACAGTCGAGAACATGCCG
ACCGGAGGCACCTTGCTGCGGTGGTCTGCACCGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGGCTGCACCCTGCTGGTCGTCGGTCAGCAGTATCTGTGACTGTGCCGCTGGGTAGCCAGACCAGTTGGTTGAGCGCAGC
TCCGGTGTCAACATAGCTAT

Downstream 100 bases:

>100_bases
TGGATTGATGGACTGTGTTCCGACCGCAAGGAGCGTGAAATGTCTGCTGCGACAGCGAAATACGGAATACTCGTTGGCGT
CGACGGATCAGCGCAATCCA

Product: histidine kinase response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTHPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGLKLDATLRAIVHTAAELVDAR
YGALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLGALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRI
RDEVFGNLYLTEKADGQPFSDDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEE
ALTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDRTPRRFDRLDLAVDGPVEPGP
ALVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFADQAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLF
AVGLTLQGAAPRARVPAVRESIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLS
VVDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGLRNLRQRADDAGGEFTVENMP
TGGTLLRWSAPLR

Sequences:

>Translated_573_residues
MTHPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGLKLDATLRAIVHTAAELVDAR
YGALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLGALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRI
RDEVFGNLYLTEKADGQPFSDDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEE
ALTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDRTPRRFDRLDLAVDGPVEPGP
ALVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFADQAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLF
AVGLTLQGAAPRARVPAVRESIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLS
VVDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGLRNLRQRADDAGGEFTVENMP
TGGTLLRWSAPLR
>Mature_572_residues
THPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGLKLDATLRAIVHTAAELVDARY
GALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLGALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRIR
DEVFGNLYLTEKADGQPFSDDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEEA
LTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDRTPRRFDRLDLAVDGPVEPGPA
LVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFADQAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLFA
VGLTLQGAAPRARVPAVRESIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLSV
VDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGLRNLRQRADDAGGEFTVENMPT
GGTLLRWSAPLR

Specific function: Interacts with the two-component regulatory system devR/devS (dosR/dosS) involved in onset of the dormancy response. May act as a direct hypoxia sensor (rather than a redox sensor); the normal state is the oxygenated form, while the deoxy form is probably

COG id: COG2203

COG function: function code T; FOG: GAF domain

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1788812, Length=270, Percent_Identity=27.037037037037, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787474, Length=259, Percent_Identity=24.7104247104247, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DOST_MYCTU (O53473)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842578
- PIR:   B70942
- RefSeq:   NP_216543.1
- RefSeq:   NP_336551.1
- PDB:   2VZW
- PDBsum:   2VZW
- ProteinModelPortal:   O53473
- SMR:   O53473
- EnsemblBacteria:   EBMYCT00000002814
- EnsemblBacteria:   EBMYCT00000069904
- GeneID:   888471
- GeneID:   924667
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2086
- KEGG:   mtu:Rv2027c
- TIGR:   MT2086
- TubercuList:   Rv2027c
- GeneTree:   EBGT00050000014831
- HOGENOM:   HBG322367
- OMA:   NEKVDQS
- ProtClustDB:   CLSK791572
- GO:   GO:0005737
- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR011712
- Gene3D:   G3DSA:3.30.565.10
- SMART:   SM00065
- SMART:   SM00387

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF07730 HisKA_3; SSF55874 ATP_bd_ATPase

EC number: 2.7.3.- [C]

Molecular weight: Translated: 62169; Mature: 62038

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLDATLRAIVHTAAELVDARYGALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHH
ALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRIRDEVFGNLYLTEKADGQPFS
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEHHHHCCCEEEEECCCCCCCC
DDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEE
CCHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDR
HHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCC
TPRRFDRLDLAVDGPVEPGPALVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFAD
CCCHHHHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLFAVGLTLQGAAPRARVPAVRE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHH
SIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHEEECCCHH
VVDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHH
RNLRQRADDAGGEFTVENMPTGGTLLRWSAPLR
HHHHHHHCCCCCEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure 
THPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLDATLRAIVHTAAELVDARYGALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHH
ALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRIRDEVFGNLYLTEKADGQPFS
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEHHHHCCCEEEEECCCCCCCC
DDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEE
CCHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDR
HHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCC
TPRRFDRLDLAVDGPVEPGPALVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFAD
CCCHHHHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLFAVGLTLQGAAPRARVPAVRE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHH
SIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHEEECCCHH
VVDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHH
RNLRQRADDAGGEFTVENMPTGGTLLRWSAPLR
HHHHHHHCCCCCEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036