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The map label for this gene is nrdF1

Identifier: 148823195

GI number: 148823195

Start: 2232474

End: 2233442

Strand: Reverse

Name: nrdF1

Synonym: TBFG_12012

Alternate gene names: 148823195

Gene position: 2233442-2232474 (Counterclockwise)

Preceding gene: 148823196

Following gene: 148823194

Centisome position: 50.48

GC content: 59.13

Gene sequence:

>969_bases
ATGACCGGCAAGCTCGTTGAGCGGGTGCACGCAATCAATTGGAACCGGTTGCTCGATGCTAAAGATTTGCAGGTCTGGGA
ACGTTTGACCGGTAACTTTTGGTTGCCGGAAAAGATTCCGCTCTCCAACGACCTGGCATCTTGGCAAACGTTGAGTTCCA
CCGAGCAGCAGACGACGATCCGGGTGTTCACCGGCTTGACCCTGCTCGACACCGCGCAGGCGACGGTGGGAGCAGTGGCC
ATGATCGACGACGCGGTCACCCCCCACGAAGAGGCGGTCCTGACCAACATGGCGTTCATGGAGTCAGTGCACGCCAAGAG
CTACAGCTCGATCTTCTCGACCCTGTGCTCGACCAAGCAGATCGACGATGCCTTCGACTGGTCGGAACAGAACCCTTACC
TGCAGCGAAAAGCGCAGATCATCGTCGACTACTACCGCGGTGACGACGCGCTCAAGCGCAAAGCATCGTCGGTAATGCTG
GAGTCCTTCCTGTTCTACTCCGGCTTCTACCTGCCCATGTACTGGTCGTCGCGGGGTAAGCTCACCAACACCGCCGATCT
GATCCGGCTGATCATCCGAGATGAAGCCGTCCACGGCTACTACATCGGCTACAAATGTCAACGAGGTTTGGCCGACCTGA
CCGACGCCGAGCGGGCCGACCACCGCGAATACACCTGCGAGCTGCTGCACACGCTCTACGCGAACGAGATCGACTATGCG
CACGACTTGTACGACGAGTTGGGCTGGACCGACGACGTTTTGCCCTACATGCGTTACAACGCCAACAAGGCGCTAGCCAA
CCTGGGATACCAGCCTGCATTCGATCGTGACACCTGCCAGGTGAACCCGGCCGTGCGCGCAGCTCTCGACCCCGGTGCAG
GGGAGAACCACGACTTTTTCTCCGGCTCCGGAAGCTCATACGTAATGGGCACCCACCAACCCACCACCGACACCGACTGG
GACTTCTAA

Upstream 100 bases:

>100_bases
CGGCCGAAATGCCCAAGGACCGACTGCCGGACTTGACGCCCACAGGAGCGTACGCACCGGCCAACAGCGGCATGACCATG
GCAAGGCAGGACGGCCCTCG

Downstream 100 bases:

>100_bases
CCGCCCAGCGCGTCGGGGGCGTCGAGCACCACGCGACACCGGGCCCGATCGATCTGCTAGCTTGAGTCTGGTCAGGCATC
GTCGTCAGCAGCGCGATGCC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase R2-1 small subunit; Ribonucleotide reductase small subunit 1

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTIRVFTGLTLLDTAQATVGAVA
MIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQIDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVML
ESFLFYSGFYLPMYWSSRGKLTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYA
HDLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFFSGSGSSYVMGTHQPTTDTDW
DF

Sequences:

>Translated_322_residues
MTGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTIRVFTGLTLLDTAQATVGAVA
MIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQIDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVML
ESFLFYSGFYLPMYWSSRGKLTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYA
HDLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFFSGSGSSYVMGTHQPTTDTDW
DF
>Mature_321_residues
TGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTIRVFTGLTLLDTAQATVGAVAM
IDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQIDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVMLE
SFLFYSGFYLPMYWSSRGKLTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYAH
DLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFFSGSGSSYVMGTHQPTTDTDWD
F

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Two genes for this protein are present in M.tuberculosis; this is thought to not be the active form. When coexp

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=64.6687697160883, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2A_MYCTU (Q10840)

Other databases:

- EMBL:   U41099
- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   C70756
- RefSeq:   NP_336494.1
- RefSeq:   YP_177853.1
- ProteinModelPortal:   Q10840
- SMR:   Q10840
- EnsemblBacteria:   EBMYCT00000000761
- EnsemblBacteria:   EBMYCT00000069249
- GeneID:   885923
- GeneID:   923454
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2033
- KEGG:   mtu:Rv1981c
- TIGR:   MT2033
- TubercuList:   Rv1981c
- GeneTree:   EBGT00050000016987
- HOGENOM:   HBG516660
- OMA:   MMRIGVE
- ProtClustDB:   PRK13967
- BRENDA:   1.17.4.1
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 36590; Mature: 36459

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTI
CCCHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHEE
RVFTGLTLLDTAQATVGAVAMIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQ
EEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVMLESFLFYSGFYLPMYWSSRGK
CCHHCCCCCCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
LTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYA
CCHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HDLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFF
HHHHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCEEECHHHHEEECCCCCCCCCCC
SGSGSSYVMGTHQPTTDTDWDF
CCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTI
CCHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHEE
RVFTGLTLLDTAQATVGAVAMIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQ
EEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVMLESFLFYSGFYLPMYWSSRGK
CCHHCCCCCCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
LTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYA
CCHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HDLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFF
HHHHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCEEECHHHHEEECCCCCCCCCCC
SGSGSSYVMGTHQPTTDTDWDF
CCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9335290; 9634230; 12218036