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The map label for this gene is pbp [H]

Identifier: 148822936

GI number: 148822936

Start: 1952392

End: 1954011

Strand: Reverse

Name: pbp [H]

Synonym: TBFG_11747

Alternate gene names: 148822936

Gene position: 1954011-1952392 (Counterclockwise)

Preceding gene: 148822938

Following gene: 148822935

Centisome position: 44.16

GC content: 61.6

Gene sequence:

>1620_bases
TTGACGAAGCGGATCCCCCCACGAAGCTGTTCAGATCGGCCGCCACCGGCCGGGTCGCCGCGGTCCTTGTGTCCGCCGAT
CATCTTGAGTTCCGCGACGCCGACCGGCACGCGGTGCGGGACGCGCCATGGGCGCGCGGTCGTCACCGAATACGTGCGTG
CGCTAGATCGACTGCCGCACGAAATCGCCACTGCAGTGGTTGAAACTGTCAACTGCGCAGACCCTGGTGCAGCGTTCGAC
GAACTCGATGCAAAAATCAACGCGGGCATGAAGGCCTATGCGATTCCGGGCGTCGCGGTTGCTGTCTGGGCCGGCGGGCA
AGAATACGTCAAAGGCTACGGGGTCACCAATGTCGACCATCCGATGCCTGTTGACGGCGACACTGTCTTCAGAATCGGTT
CCACCACAAAGACTTTCACAGGTACGGTGATGATGCGGCTGGTCGAGCGAGGCAAGGTGGACCTGGATTCACCTGTGCGC
CGCTACATCCCCGACTTCGCGGTAGCCGACGAATCAGCCAGCGCTACGGTTACCGTTCGCCAACTGCTCAACCATACCGC
AGGCTGGGATGGTCGCAATGGGCAGGACTTTGGGCGCGGCGATGACGCGGTGGCGCTCTATGTCAAGGCGATGACACGCC
TACCGCAGCTCACCCCTCCGGGAACCGCGTTCGCGTACAACAATTCAGGTCTTGTGGTTGCGGGCCGCATCATCGAGCTT
GTCGCCGGAACAACCTACGAATCTACGGTTCAGAGGCTGTTGCTTGACCCGCTGCAGCTTGCTCACACGCGCTACTTTTC
CGACCAAATAATCGGTCTGAATGTGGCCGCATCGCATAGCGTGGTCGACGGCAAACCGATTGCCGTTACTGACTTTTGGA
CATTCCCGCGCAGCTGCAACCCCACCGGTGGGTTGATGTCCACAGCGCGAGATCAGCTGCGTTACGCACAGTTCCACCTC
GGCGACGGCAGGGCGCCTAACGGTGAGCAGATTCTGAGCCGACAATCGCTGAAGGCAATGCGCTCTAACCCTGGGGCGGG
CGGAACACTTTGGGTGGAACTGACCGGGATGGGCGTGACCTGGATGCTGCGGCCCTCCGCGGAGAATGTGACCATCGTTG
AGCACGGCGGCACCTGGAAGGGGCAGCGCTCTGGGTTCGTCATGGTGCCCGATCGAAACTTCGCCATGACCGTGCTCACT
AACTCTGATGGCGGATTTCATATGATCAACGACCTTTTCGCATCCGACTGGGCATTGCAGAGATTCGCCGGGCTCAGCAA
TCTTCCGGCCACGCCGCAACGCCTTGGTGCCGTCGACCTGGCGCCCTACGAGGGCCGGTACATCGCCAAGCAAGTCGCCC
AAAATGGCGACCTCGAGACAACGGTCATCGACTTCCGGGCCAGGGACGGCCAGCTTGCTGGAAGCATGAGCACCGACGAT
GCCAACCCGGATGGCCAAAACAGCGCCAATCTGGGCCTCGCCTTCTATCGGCCCGACTATGGGCTCGACCTTGGACCCGA
CAACAAGCCCACCGGCAGTCGCTCCAACTTCGTGCGCGGGCCGGACGGCAACATCGCCTGGTTCTGTAGCCAGCACGGCC
GTCTGTTCCGACGCCAATAG

Upstream 100 bases:

>100_bases
ACCACGAAGAACCGGCCTCGCGATGTTCATGTTAGTCCAGCTATATTGACACTCATGTGGACGCGACCGCGGAAGGCCAA
AGCCATGCTTTCCGCGTTGG

Downstream 100 bases:

>100_bases
CACAGCACCAGCCGGCTTGGTTTGCGGCGCGAGGAGGAACCGGGCAATCAGACGACGCCCGGTGACGATGCGCGCCGTTT
GCGGCGCGAGGAGGAACCGG

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP-4 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTKRIPPRSCSDRPPPAGSPRSLCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFD
ELDAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVR
RYIPDFAVADESASATVTVRQLLNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIEL
VAGTTYESTVQRLLLDPLQLAHTRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHL
GDGRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLT
NSDGGFHMINDLFASDWALQRFAGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDD
ANPDGQNSANLGLAFYRPDYGLDLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ

Sequences:

>Translated_539_residues
MTKRIPPRSCSDRPPPAGSPRSLCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFD
ELDAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVR
RYIPDFAVADESASATVTVRQLLNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIEL
VAGTTYESTVQRLLLDPLQLAHTRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHL
GDGRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLT
NSDGGFHMINDLFASDWALQRFAGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDD
ANPDGQNSANLGLAFYRPDYGLDLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
>Mature_538_residues
TKRIPPRSCSDRPPPAGSPRSLCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPHEIATAVVETVNCADPGAAFDE
LDAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDHPMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRR
YIPDFAVADESASATVTVRQLLNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIELV
AGTTYESTVQRLLLDPLQLAHTRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCNPTGGLMSTARDQLRYAQFHLG
DGRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVTWMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTN
SDGGFHMINDLFASDWALQRFAGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDDA
NPDGQNSANLGLAFYRPDYGLDLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ

Specific function: Involved in cell wall biosynthesis and may also act as a sensor of external penicillins [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051233, Length=95, Percent_Identity=37.8947368421053, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI26051231, Length=95, Percent_Identity=37.8947368421053, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790593, Length=263, Percent_Identity=26.9961977186312, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786574, Length=357, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533026, Length=197, Percent_Identity=28.4263959390863, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17550934, Length=188, Percent_Identity=29.2553191489362, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 58159; Mature: 58028

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRIPPRSCSDRPPPAGSPRSLCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPH
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHH
EIATAVVETVNCADPGAAFDELDAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDH
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCHHHHHCCCCCCCCC
PMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVR
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEECCCCCCHHHHHH
QLLNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIEL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHH
VAGTTYESTVQRLLLDPLQLAHTRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCN
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
PTGGLMSTARDQLRYAQFHLGDGRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVT
CCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
WMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQ
EEECCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
RFAGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDD
HHCCCCCCCCCHHHCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCC
ANPDGQNSANLGLAFYRPDYGLDLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
TKRIPPRSCSDRPPPAGSPRSLCPPIILSSATPTGTRCGTRHGRAVVTEYVRALDRLPH
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHH
EIATAVVETVNCADPGAAFDELDAKINAGMKAYAIPGVAVAVWAGGQEYVKGYGVTNVDH
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCHHHHHCCCCCCCCC
PMPVDGDTVFRIGSTTKTFTGTVMMRLVERGKVDLDSPVRRYIPDFAVADESASATVTVR
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEECCCCCCHHHHHH
QLLNHTAGWDGRNGQDFGRGDDAVALYVKAMTRLPQLTPPGTAFAYNNSGLVVAGRIIEL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHH
VAGTTYESTVQRLLLDPLQLAHTRYFSDQIIGLNVAASHSVVDGKPIAVTDFWTFPRSCN
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
PTGGLMSTARDQLRYAQFHLGDGRAPNGEQILSRQSLKAMRSNPGAGGTLWVELTGMGVT
CCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
WMLRPSAENVTIVEHGGTWKGQRSGFVMVPDRNFAMTVLTNSDGGFHMINDLFASDWALQ
EEECCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
RFAGLSNLPATPQRLGAVDLAPYEGRYIAKQVAQNGDLETTVIDFRARDGQLAGSMSTDD
HHCCCCCCCCCHHHCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCC
ANPDGQNSANLGLAFYRPDYGLDLGPDNKPTGSRSNFVRGPDGNIAWFCSQHGRLFRRQ
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]