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The map label for this gene is hemK [H]

Identifier: 148822518

GI number: 148822518

Start: 1460332

End: 1461309

Strand: Direct

Name: hemK [H]

Synonym: TBFG_11327

Alternate gene names: 148822518

Gene position: 1460332-1461309 (Clockwise)

Preceding gene: 148822517

Following gene: 148822519

Centisome position: 33.01

GC content: 65.64

Gene sequence:

>978_bases
ATGACCTCCGCGCCGGCGACGATGCGGTGGGGGAACCTCCCGCTTGCGGGGGAGAGCGGCACAATGACCCTGCGTCAGGC
GATCGACTTGGCTGCTGCGCTATTGGCCGAAGCGGGGGTCGACTCGGCGCGTTGCGACGCTGAGCAGTTGGCCGCTCACC
TAGCGGGCACAGACCGCGGTAGGCTACCCCTGTTCGAGCCGCCCGGCGACGAGTTCTTCGGGCGCTATCGCGACATCGTC
ACCGCTCGTGCGCGGCGGGTGCCGTTGCAGCATCTCATCGGGACTGTGTCGTTTGGGCCCGTGGTGCTGCATGTCGGCCC
GGGTGTGTTTGTACCGCGTCCGGAGACCGAAGCCATTTTGGCCTGGGCCACCGCGCAGTCGCTGCCGGCGCGGCCGCTGA
TTGTCGACGCATGCACGGGATCTGGCGCGTTGGCGGTCGCATTGGCCCAGCACCGGGCCAACCTTGGACTAAAGGCCCGC
ATCATCGGCATTGACGACTCCGACTGCGCCCTTGACTATGCCCGCCGCAATGCGGCGGGTACCCCGGTAGAGTTGGTGCG
TGCCGACGTCACCACGCCCTGCCTGCTCCCCGAACTCGACGGACAAGTCGACCTGATGGTTTCCAACCCGCCCTACATCC
CTGATGCTGCTGTTTTGGAACCTGAAGTAGCGCAACATGACCCGCATCACGCGTTGTTCGGCGGTCCCGACGGGATGACG
GTGATATCCGCGGTCGTCGGGCTTGCTGGGCGCTGGCTGCGTCCCGGTGGCCTGTTCGCCGTCGAACACGACGACACCAC
GTCGTCGTCAACTGTCGATTTGGTCAGCAGCACAAAACTTTTCGTGGACGTACAAGCCCGGAAAGATCTGGCCGGACGGC
CGAGGTTTGTGACGGCGATGAGGTGGGGGCACCTCCCGCTTGCAGGGGAGAACGGCGCCATTGACCCGCGCCAGCGACGA
TGCAGAGCGAAGCGATGA

Upstream 100 bases:

>100_bases
GTTACAAGTCACACAATCTCGATCAGGTGCTGGATGGCGATCTTGACGCGTTGTTCGACGCTCTGTCCGCCGCGGACAAG
CAATCCCGGTTGCGACAATC

Downstream 100 bases:

>100_bases
GGAGAAGCGGCGCCATTGACTGAGACGTTCGACTGCGCCGACCCCGAGCAGCGTTCGCGTGGAATCGTCTCTGCGGTAGG
GGCAATCAAGGCGGGCCAAC

Product: hypothetical protein

Products: NA

Alternate protein names: M.MtuHHemKP [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTSAPATMRWGNLPLAGESGTMTLRQAIDLAAALLAEAGVDSARCDAEQLAAHLAGTDRGRLPLFEPPGDEFFGRYRDIV
TARARRVPLQHLIGTVSFGPVVLHVGPGVFVPRPETEAILAWATAQSLPARPLIVDACTGSGALAVALAQHRANLGLKAR
IIGIDDSDCALDYARRNAAGTPVELVRADVTTPCLLPELDGQVDLMVSNPPYIPDAAVLEPEVAQHDPHHALFGGPDGMT
VISAVVGLAGRWLRPGGLFAVEHDDTTSSSTVDLVSSTKLFVDVQARKDLAGRPRFVTAMRWGHLPLAGENGAIDPRQRR
CRAKR

Sequences:

>Translated_325_residues
MTSAPATMRWGNLPLAGESGTMTLRQAIDLAAALLAEAGVDSARCDAEQLAAHLAGTDRGRLPLFEPPGDEFFGRYRDIV
TARARRVPLQHLIGTVSFGPVVLHVGPGVFVPRPETEAILAWATAQSLPARPLIVDACTGSGALAVALAQHRANLGLKAR
IIGIDDSDCALDYARRNAAGTPVELVRADVTTPCLLPELDGQVDLMVSNPPYIPDAAVLEPEVAQHDPHHALFGGPDGMT
VISAVVGLAGRWLRPGGLFAVEHDDTTSSSTVDLVSSTKLFVDVQARKDLAGRPRFVTAMRWGHLPLAGENGAIDPRQRR
CRAKR
>Mature_324_residues
TSAPATMRWGNLPLAGESGTMTLRQAIDLAAALLAEAGVDSARCDAEQLAAHLAGTDRGRLPLFEPPGDEFFGRYRDIVT
ARARRVPLQHLIGTVSFGPVVLHVGPGVFVPRPETEAILAWATAQSLPARPLIVDACTGSGALAVALAQHRANLGLKARI
IGIDDSDCALDYARRNAAGTPVELVRADVTTPCLLPELDGQVDLMVSNPPYIPDAAVLEPEVAQHDPHHALFGGPDGMTV
ISAVVGLAGRWLRPGGLFAVEHDDTTSSSTVDLVSSTKLFVDVQARKDLAGRPRFVTAMRWGHLPLAGENGAIDPRQRRC
RAKR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=314, Percent_Identity=30.2547770700637, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1787463, Length=231, Percent_Identity=32.4675324675325, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582226, Length=280, Percent_Identity=29.6428571428571, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 34537; Mature: 34406

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAPATMRWGNLPLAGESGTMTLRQAIDLAAALLAEAGVDSARCDAEQLAAHLAGTDRG
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCC
RLPLFEPPGDEFFGRYRDIVTARARRVPLQHLIGTVSFGPVVLHVGPGVFVPRPETEAIL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCEECCCCCCCEEE
AWATAQSLPARPLIVDACTGSGALAVALAQHRANLGLKARIIGIDDSDCALDYARRNAAG
EEEECCCCCCCCEEEEECCCCCCEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCC
TPVELVRADVTTPCLLPELDGQVDLMVSNPPYIPDAAVLEPEVAQHDPHHALFGGPDGMT
CCHHHEEECCCCCEECCCCCCCEEEEECCCCCCCCCHHCCCHHHCCCCCCEECCCCCHHH
VISAVVGLAGRWLRPGGLFAVEHDDTTSSSTVDLVSSTKLFVDVQARKDLAGRPRFVTAM
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEEEEECCCCCCCCCEEEEE
RWGHLPLAGENGAIDPRQRRCRAKR
ECCCCEECCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TSAPATMRWGNLPLAGESGTMTLRQAIDLAAALLAEAGVDSARCDAEQLAAHLAGTDRG
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCC
RLPLFEPPGDEFFGRYRDIVTARARRVPLQHLIGTVSFGPVVLHVGPGVFVPRPETEAIL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCEECCCCCCCEEE
AWATAQSLPARPLIVDACTGSGALAVALAQHRANLGLKARIIGIDDSDCALDYARRNAAG
EEEECCCCCCCCEEEEECCCCCCEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCC
TPVELVRADVTTPCLLPELDGQVDLMVSNPPYIPDAAVLEPEVAQHDPHHALFGGPDGMT
CCHHHEEECCCCCEECCCCCCCEEEEECCCCCCCCCHHCCCHHHCCCCCCEECCCCCHHH
VISAVVGLAGRWLRPGGLFAVEHDDTTSSSTVDLVSSTKLFVDVQARKDLAGRPRFVTAM
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEEEEECCCCCCCCCEEEEE
RWGHLPLAGENGAIDPRQRRCRAKR
ECCCCEECCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]