Click here to switch to the map view.

The map label for this gene is cysNC [H]

Identifier: 148822503

GI number: 148822503

Start: 1442674

End: 1444518

Strand: Direct

Name: cysNC [H]

Synonym: TBFG_11312

Alternate gene names: 148822503

Gene position: 1442674-1444518 (Clockwise)

Preceding gene: 148822502

Following gene: 148822504

Centisome position: 32.61

GC content: 63.25

Gene sequence:

>1845_bases
ATGACGACGCTATTGCGGCTGGCGACAGCGGGTTCCGTCGACGATGGCAAGTCCACGCTGATTGGGCGGCTACTCTACGA
CTCCAAGGCTGTGATGGAAGACCAGTGGGCGTCGGTGGAGCAAACGTCCAAGGACCGGGGCCACGACTACACCGACCTGG
CTCTGGTCACCGACGGCCTGCGGGCCGAGCGGGAACAGGGCATCACCATCGACGTTGCCTACCGCTACTTCGCCACTCCC
AAGCGGAAATTCATCATTGCCGACACCCCGGGACACATCCAATACACCCGCAACATGGTGACCGGTGCGTCCACCGCCCA
ACTGGTGATCGTACTGGTGGATGCCCGGCACGGCTTGCTGGAGCAATCCCGCCGGCACGCCTTCCTGGCGTCGCTGCTGG
GCATCCGCCACCTGGTGCTCGCGGTCAACAAGATGGACTTGCTTGGCTGGGACCAAGAGAAATTCGACGCGATTCGAGAC
GAATTCCACGCCTTCGCGGCCCGCCTCGACGTGCAGGACGTCACCTCCATCCCAATCTCCGCGCTGCACGGCGACAACGT
GGTGACCAAATCCGACCAGACGCCCTGGTACGAGGGACCGTCGCTGCTGTCGCATCTCGAAGACGTCTACATCGCCGGTG
ACCGCAACATGGTCGACGTGCGATTCCCGGTCCAGTACGTCATCCGGCCGCACACCCTCGAGCATCAAGACCACCGCAGC
TACGCGGGCACCGTGGCCAGTGGGGTAATGCGTTCAGGCGACGAAGTTGTCGTGCTGCCGATCGGTAAGACCACCCGGAT
CACCGCGATCGACGGCCCGAACGGCCCGGTGGCAGAAGCGTTTCCGCCGATGGCGGTTTCGGTGCGGCTCGCCGACGACA
TCGATATCTCGCGTGGTGACATGATCGCTCGCACCCACAACCAGCCCAGGATCACACAAGAATTCGACGCGACCGTGTGC
TGGATGGCCGACAACGCGGTGCTAGAGCCCGGCCGCGACTACGTTGTCAAGCACACCACCCGAACCGTCCGCGCGAGGAT
AGCCGGGCTGGATTACCGGCTCGATGTCAACACCCTGCATCGCGACAAGACCGCAACGGCGTTGAAACTCAACGAACTGG
GCCGTGTTTCGCTGCGCACCCAGGTGCCGTTGCTGCTTGACGAGTACACCCGCAACGCTAGCACCGGCTCGTTCATCCTC
ATTGACCCCGACACCAACGGAACGGTGGCGGCGGGCATGGTGTCACGCGACGTCTCGGCCCGCACGCCTAGCCCGAACAC
GGTGCGGCACAGATCGCTCGTCACTGCGCAAGATCGGCCGCCCAGGGGCAAGACGGTGTGGTTTACCGGACTGTCCGGCT
CCGGCAAGTCGTCGGTGGCCATGCTGGTTGAGCGGAAGCTACTCGAAAAGGGCATCTCCGCTTACGTTCTGGACGGCGAC
AACCTACGGCATGGCCTCAACGCCGACCTGGGCTTTTCCATGGCCGACCGCGCGGAGAACCTGCGCCGGCTGTCGCATGT
GGCCACACTGCTCGCCGATTGTGGCCACCTGGTGCTGGTGCCGGCGATCAGCCCCCTTGCTGAGCACCGTGCCCTGGCTC
GTAAAGTGCACGCTGATGCGGGAATCGACTTTTTCGAGGTGTTCTGTGACACCCCGCTGCAGGACTGTGAGAGGCGTGAT
CCCAAAGGGTTGTACGCCAAAGCGCGTGCGGGTGAGATCACGCACTTCACCGGGATCGACAGCCCATATCAGCGGCCCAA
GAACCCAGACCTACGGCTTACGCCGGATCGCAGCATAGACGAGCAGGCGCAGGAGGTTATCGACCTGTTGGAGTCATCGT
CTTAG

Upstream 100 bases:

>100_bases
AAACTGCGGTGGCCCGCTTGACGGAGCGCGGGGCGACCAGGGCTGACGACCGGATCTCGGAGGCTGGAATGGAAGACCGC
AAGCGGCAGGGATACTTCTG

Downstream 100 bases:

>100_bases
GCCGGCCTGGTTGCTCTGCTGTCCCTGGCAAGCGGGTGGCACAATCCTGAAGCATGCGGATGTCAGCTAAGGCGGAGTAC
GCGGTGCGGGCGATGGTCCA

Product: bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase protein

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MTTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGLRAEREQGITIDVAYRYFATP
KRKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLLEQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRD
EFHAFAARLDVQDVTSIPISALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRS
YAGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGDMIARTHNQPRITQEFDATVC
WMADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLHRDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFIL
IDPDTNGTVAAGMVSRDVSARTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGD
NLRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADAGIDFFEVFCDTPLQDCERRD
PKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSIDEQAQEVIDLLESSS

Sequences:

>Translated_614_residues
MTTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGLRAEREQGITIDVAYRYFATP
KRKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLLEQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRD
EFHAFAARLDVQDVTSIPISALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRS
YAGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGDMIARTHNQPRITQEFDATVC
WMADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLHRDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFIL
IDPDTNGTVAAGMVSRDVSARTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGD
NLRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADAGIDFFEVFCDTPLQDCERRD
PKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSIDEQAQEVIDLLESSS
>Mature_613_residues
TTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGLRAEREQGITIDVAYRYFATPK
RKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLLEQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRDE
FHAFAARLDVQDVTSIPISALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRSY
AGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGDMIARTHNQPRITQEFDATVCW
MADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLHRDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFILI
DPDTNGTVAAGMVSRDVSARTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGDN
LRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADAGIDFFEVFCDTPLQDCERRDP
KGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSIDEQAQEVIDLLESSS

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=172, Percent_Identity=52.9069767441861, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI62912492, Length=174, Percent_Identity=50.5747126436782, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI34447231, Length=174, Percent_Identity=50.5747126436782, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI4503475, Length=439, Percent_Identity=27.1070615034169, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI223555963, Length=428, Percent_Identity=29.9065420560748, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI5729864, Length=428, Percent_Identity=29.9065420560748, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI194018522, Length=428, Percent_Identity=26.6355140186916, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI194018520, Length=428, Percent_Identity=26.6355140186916, Blast_Score=157, Evalue=4e-38,
Organism=Homo sapiens, GI194097354, Length=428, Percent_Identity=26.6355140186916, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI46094014, Length=428, Percent_Identity=25.7009345794392, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4503471, Length=439, Percent_Identity=26.6514806378132, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI34147630, Length=320, Percent_Identity=25.625, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1789108, Length=427, Percent_Identity=49.8829039812646, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1789107, Length=193, Percent_Identity=50.7772020725389, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1790412, Length=143, Percent_Identity=30.0699300699301, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=143, Percent_Identity=30.0699300699301, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17542422, Length=173, Percent_Identity=50.8670520231214, Blast_Score=179, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17552884, Length=433, Percent_Identity=28.4064665127021, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=433, Percent_Identity=28.4064665127021, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=431, Percent_Identity=26.4501160092807, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=217, Percent_Identity=35.0230414746544, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=217, Percent_Identity=35.0230414746544, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=426, Percent_Identity=26.5258215962441, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=142, Percent_Identity=37.3239436619718, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25141371, Length=262, Percent_Identity=26.7175572519084, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=147, Percent_Identity=32.6530612244898, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322852, Length=184, Percent_Identity=50, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=27.8538812785388, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=27.8538812785388, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=26.6666666666667, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=431, Percent_Identity=28.538283062645, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324761, Length=145, Percent_Identity=34.4827586206897, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667032, Length=173, Percent_Identity=52.6011560693642, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24667028, Length=173, Percent_Identity=52.6011560693642, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24667036, Length=173, Percent_Identity=52.6011560693642, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI116007838, Length=173, Percent_Identity=52.6011560693642, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24667044, Length=173, Percent_Identity=52.6011560693642, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24667040, Length=172, Percent_Identity=52.9069767441861, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45553807, Length=433, Percent_Identity=27.0207852193995, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553816, Length=433, Percent_Identity=27.0207852193995, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24651721, Length=433, Percent_Identity=27.0207852193995, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17864154, Length=433, Percent_Identity=27.0207852193995, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24652838, Length=437, Percent_Identity=26.5446224256293, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137572, Length=437, Percent_Identity=26.5446224256293, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137380, Length=422, Percent_Identity=25.8293838862559, Blast_Score=146, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.8990825688073, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281363316, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17864358, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921738, Length=207, Percent_Identity=28.9855072463768, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 67814; Mature: 67682

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGL
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCEEHEEEECCH
RAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLL
HHHHHCCCEEEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCCCCEEEEEEEEECCCCHH
EQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRDEFHAFAARLDVQDVTSIPIS
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
ALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRS
EECCCCEEECCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCEEEEECCCCCCCCCCHH
YAGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGD
HHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCC
MIARTHNQPRITQEFDATVCWMADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLH
EEEECCCCCCCCCCCCCEEEEEECCCEECCCCCEEEEHHHHHHHHHHCCCEEEEECHHHC
RDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFILIDPDTNGTVAAGMVSRDVSA
CCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEEHCCCCCC
RTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGD
CCCCCCCEECCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCC
NLRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCC
GIDFFEVFCDTPLQDCERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSID
CCHHHHHHHCCCHHHHHCCCCCCCEEECCCCCEEEECCCCCHHCCCCCCCEEECCCCCHH
EQAQEVIDLLESSS
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGL
CCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCEEHEEEECCH
RAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLL
HHHHHCCCEEEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCCCCEEEEEEEEECCCCHH
EQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRDEFHAFAARLDVQDVTSIPIS
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
ALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRS
EECCCCEEECCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCEEEEECCCCCCCCCCHH
YAGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGD
HHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCC
MIARTHNQPRITQEFDATVCWMADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLH
EEEECCCCCCCCCCCCCEEEEEECCCEECCCCCEEEEHHHHHHHHHHCCCEEEEECHHHC
RDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFILIDPDTNGTVAAGMVSRDVSA
CCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEEHCCCCCC
RTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGD
CCCCCCCEECCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCC
NLRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCC
GIDFFEVFCDTPLQDCERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSID
CCHHHHHHHCCCHHHHHCCCCCCCEEECCCCCEEEECCCCCHHCCCCCCCEEECCCCCHH
EQAQEVIDLLESSS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]