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The map label for this gene is holB [H]

Identifier: 148663507

GI number: 148663507

Start: 4089963

End: 4091168

Strand: Reverse

Name: holB [H]

Synonym: MRA_3679

Alternate gene names: 148663507

Gene position: 4091168-4089963 (Counterclockwise)

Preceding gene: 148663509

Following gene: 148663505

Centisome position: 92.56

GC content: 69.73

Gene sequence:

>1206_bases
ATGTCCGGGGTGTTTACGCGGCTGGTAGGCCAGCAAGCGGTGGAAGCCGAGCTGCTTGCCACGGCCAAGGCGGCCCGTCG
TGATTCGGCTCACAGCGCGGGTGGCGGCGGGACTATGACACACGCGTGGCTGCTCACCGGTCCGCCCGGTTCTGGGCGCT
CGGTTGCCGCGCTGTGCTTCGCGGCGGCTTTGCAATGTACGTCGGGCGGGGAGCCCGGGTGCGGGCGCTGCCGGGCGTGT
ACGACCACGCTGGCGGGCACCCACGCCGACGTACGCCGGGTGATTCCCGAAGGTCTGTCGATCGGTGTGGACGAAATGCG
GGCCATCGTGCAAATCGCCGCGCGCCGGCCGACTACCGGGCACTGGCAGATCGTGGTGATTGAGGACGCCGATCGGTTGA
CCGAAGGCGCCGCCAACGCGTTGCTCAAGGTTGTCGAGGAGCCGCCGCCGTCGACGGTGTTCCTGCTGTGCGCGCCGTCG
GTGGATCCCGAGGACATTGCGGTCACGCTGCGATCCCGGTGTAGGCATGTCGCGTTGGTGACTCCGTCGACCCACGCGAT
CGCGCAGGTGCTGAGCGACGGCGACGGGTTGGACCCCGACACGGCCAATTGGGCGGCGTCGGTCAGTGGTGGCCATGTCG
GACGAGCGCGCCGGCTGGCCACCGATCCGCAGGCCCGGCAGCGACGCGAGCGGGCTTTGGGGCTGGCACGTGACGCCGCG
ACACCCTCGCGTGCCTACGCAGCCGCCGAGGAGTTGGTGGCCGGCGCCGAGGCCGAGGCCTTAGCGTTGACCGCGCAGCG
TATCGAGGCCGAGACCGAAGAACTGCGGACGGCGCTGGGAGCCGGAGGTACCGGTAAAGGCACCGGCGCGGCGTTGCGTG
GTGCCACCGGCGCGATGAAGGATCTTGAACGACGGCAGAAATCCCGGCAGACGCGGGCCTCGCGCGATGCGCTGGACCGG
GCACTGATCGATCTGGCGACCTACTTCCGGGATGCACTGTTGGTCGCAGCCCACGCGGGTGGGGTGCGGGCCAACCACCC
GGATATGGCCGACCGGGTTGCTGCGCTGGCCGCCCACGCCCCGCCGGAGCGGCTGCTGCGCTGCATCGAAGCGGTGTTGG
CGTGCAGGGAAGCGCTAGCGGTCAACGTCAAACCGAAGTTCGCCGTCGATGCCATGGTCGCCACCATCGGCCAGGAACTG
CGGTAG

Upstream 100 bases:

>100_bases
CGCCTCGGCATCCATCCAAATGGCGACGAAAGATCACGGAATTGTCGCGAAACGAACGCTGATCGGTATCTGTCGGCGCG
GATAGCTACTGTTGCCGATG

Downstream 100 bases:

>100_bases
CGACCGCCGCAAACTTGGGCGGGCCGGCCCGCCTGCCGTAGACTCGTGCCGCCCGGCAAGCGGGCACCCAGGGGAAAAGC
CGCCTTAGCTCAGTCGGTAG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCFAAALQCTSGGEPGCGRCRAC
TTTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTGHWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
VDPEDIAVTLRSRCRHVALVTPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAA
TPSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMKDLERRQKSRQTRASRDALDR
ALIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHAPPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQEL
R

Sequences:

>Translated_401_residues
MSGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCFAAALQCTSGGEPGCGRCRAC
TTTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTGHWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
VDPEDIAVTLRSRCRHVALVTPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAA
TPSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMKDLERRQKSRQTRASRDALDR
ALIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHAPPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQEL
R
>Mature_400_residues
SGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCFAAALQCTSGGEPGCGRCRACT
TTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTGHWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSV
DPEDIAVTLRSRCRHVALVTPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAAT
PSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMKDLERRQKSRQTRASRDALDRA
LIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHAPPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQELR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=162, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787341, Length=166, Percent_Identity=36.144578313253, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41785; Mature: 41654

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTSGGEPGCGRCRACTTTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTG
HHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
HWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSVDPEDIAVTLRSRCRHVALV
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEE
TPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAA
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCC
TPSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMK
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHH
DLERRQKSRQTRASRDALDRALIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQELR
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTSGGEPGCGRCRACTTTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTG
HHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
HWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSVDPEDIAVTLRSRCRHVALV
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEE
TPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAA
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCC
TPSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMK
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHH
DLERRQKSRQTRASRDALDRALIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQELR
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]