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The map label for this gene is folK

Identifier: 148663470

GI number: 148663470

Start: 4056846

End: 4057412

Strand: Reverse

Name: folK

Synonym: MRA_3645

Alternate gene names: 148663470

Gene position: 4057412-4056846 (Counterclockwise)

Preceding gene: 148663471

Following gene: 148663469

Centisome position: 91.8

GC content: 67.37

Gene sequence:

>567_bases
ATGACGCGGGTAGTGCTCTCGGTTGGCTCCAACCTGGGTGACCGCCTGGCACGATTGCGGTCGGTCGCCGACGGTCTCGG
CGATGCGTTGATTGCGGCTTCCCCGATATATGAGGCCGACCCCTGGGGTGGGGTGGAGCAGGGGCAGTTCCTCAATGCGG
TGCTGATCGCCGACGATCCTACCTGCGAACCGCGGGAGTGGCTGCGGCGGGCGCAGGAGTTCGAGCGCGCTGCGGGCAGG
GTGCGTGGCCAGCGCTGGGGTCCACGAAATCTCGACGTCGACCTGATCGCCTGCTACCAGACCTCGGCCACCGAGGCTCT
GGTCGAAGTGACCGCGCGGGAGAACCACCTCACGCTGCCGCACCCACTGGCGCATCTGCGGGCCTTTGTGTTGATCCCGT
GGATTGCCGTCGACCCAACGGCGCAGCTGACGGTTGCCGGGTGCCCGCGGCCCGTCACGCGACTGCTGGCCGAGCTGGAG
CCCGCCGACCGCGACAGTGTGCGGTTGTTTAGGCCGTCGTTCGATCTGAATAGCAGACACCCCGTCAGTCGGGCACCGGA
AAGCTGA

Upstream 100 bases:

>100_bases
CGCAGGCGCCCATTCCGCAGACGTTCGACGATGTGGCGGTGGTGATCCGACGCTCACGGCGCGGCGGCCGCGGTTGGGTA
GTCCCGGCGGGCGGCGCGGT

Downstream 100 bases:

>100_bases
TGGGACCGACCCGAAAACGTGATCTGACCGCCGCGGTGGTCGGCGCCGCGGCGGTGGGATATCTGTTGGTGGCGGTGCTT
TACCGGTGGTTTCCGCCGAT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTRVVLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDPTCEPREWLRRAQEFERAAGR
VRGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLPHPLAHLRAFVLIPWIAVDPTAQLTVAGCPRPVTRLLAELE
PADRDSVRLFRPSFDLNSRHPVSRAPES

Sequences:

>Translated_188_residues
MTRVVLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDPTCEPREWLRRAQEFERAAGR
VRGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLPHPLAHLRAFVLIPWIAVDPTAQLTVAGCPRPVTRLLAELE
PADRDSVRLFRPSFDLNSRHPVSRAPES
>Mature_187_residues
TRVVLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDPTCEPREWLRRAQEFERAAGRV
RGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLPHPLAHLRAFVLIPWIAVDPTAQLTVAGCPRPVTRLLAELEP
ADRDSVRLFRPSFDLNSRHPVSRAPES

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family

Homologues:

Organism=Escherichia coli, GI1786335, Length=144, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPPK_MYCBO (P64144)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857275.1
- ProteinModelPortal:   P64144
- SMR:   P64144
- EnsemblBacteria:   EBMYCT00000018084
- GeneID:   1093138
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3636c
- GeneTree:   EBGT00050000016430
- HOGENOM:   HBG662565
- OMA:   ILACKAS
- ProtClustDB:   CLSK792592
- BioCyc:   MBOV233413:MB3636C-MONOMER
- BRENDA:   2.7.6.3
- InterPro:   IPR000550
- Gene3D:   G3DSA:3.30.70.560
- TIGRFAMs:   TIGR01498

Pfam domain/function: PF01288 HPPK; SSF55083 Hppk

EC number: =2.7.6.3

Molecular weight: Translated: 20732; Mature: 20601

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVVLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDP
CCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
TCEPREWLRRAQEFERAAGRVRGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
HPLAHLRAFVLIPWIAVDPTAQLTVAGCPRPVTRLLAELEPADRDSVRLFRPSFDLNSRH
CHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
PVSRAPES
CCCCCCCC
>Mature Secondary Structure 
TRVVLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDP
CEEEEECCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
TCEPREWLRRAQEFERAAGRVRGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
HPLAHLRAFVLIPWIAVDPTAQLTVAGCPRPVTRLLAELEPADRDSVRLFRPSFDLNSRH
CHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
PVSRAPES
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972