| Definition | Mycobacterium tuberculosis H37Ra, complete genome. |
|---|---|
| Accession | NC_009525 |
| Length | 4,419,977 |
Click here to switch to the map view.
The map label for this gene is hdhA [C]
Identifier: 148663393
GI number: 148663393
Start: 3975704
End: 3976486
Strand: Reverse
Name: hdhA [C]
Synonym: MRA_3569
Alternate gene names: 148663393
Gene position: 3976486-3975704 (Counterclockwise)
Preceding gene: 148663394
Following gene: 148663392
Centisome position: 89.97
GC content: 62.58
Gene sequence:
>783_bases ATGACCGGGATGCTCAAGCGCAAGGTGATCGTCGTCAGTGGCGTCGGACCGGGGCTGGGCACGACGCTGGCGCACCGATG TGCGCGAGACGGCGCCGATCTGGTGCTGGCTGCGCGCAGCGCGGAGCGTCTCGACGACGTCGCCAAGCAGATCATCGACA CCGGGCGACGGGCGGTGGCGGTGCGCACCGACATCACCGACGACGACGACGTAAGCAACCTTGTGCAGGCCACGCTGGCG GCGTACGGCAAGGCCGACGTGCTGATCAACAACGCGTTCCGGGTGCCATCGATGAAACCATTGGCCGGCACCACATTTGA GCACATCCGTGACGCGATAGAGCTCAGCGCGCTGGGGACGCTACGACTCATCCAGGCGTTTACGCCCGCGCTGGCGCAGT CTCACGGCGCGATCGTCAACGTCAACTCCATGGTGATCCGGCACTCGCAGCCGAAATACGGCACCTACAAGATGGCCAAG TCCGTACTGCTAGCTATGTCGCATTCGCTGGCCACGGAACTGGGCGAGCAGGGAATTCGCGTCAATTCCGTTGCACCCGG CTATATCTGGGGTGACACGCTGAAAAGCTATTTCGACCATCAGGCCGGCAAATACGGCACTACCGTGGATCAGATCTATC AGGCGACCGCGGCAAACTCCGATCTCAAACGGCTGCCAACCGAAGACGAGGTGGCCTCAGCGATTCTTTTCCTGGCCAGC GACCTGGCCAGCGGCATCACCGGGCAGACCCTGGACGTCAATTGCGGGGAGTACCACACGTAA
Upstream 100 bases:
>100_bases CGGCGGCACTGCCGCATTACCAGCACAACAAGATTTCCGAGGATGATTGGCGGGCGCGAATTGCGTTGCGTCAACGGCAA ATCGCGACCAGGATGCTGGG
Downstream 100 bases:
>100_bases TGACTCGGCGTCCCGATCGGAAAGATGTGGCCACCGTCGACGAACTGCACGCATCGGCTACCAAACTGGTGGGTCTCGAC GATTTTGGCACCGACGACGA
Product: short chain dehydrogenase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: ORFC [H]
Number of amino acids: Translated: 260; Mature: 259
Protein sequence:
>260_residues MTGMLKRKVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVAVRTDITDDDDVSNLVQATLA AYGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGTLRLIQAFTPALAQSHGAIVNVNSMVIRHSQPKYGTYKMAK SVLLAMSHSLATELGEQGIRVNSVAPGYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSDLKRLPTEDEVASAILFLAS DLASGITGQTLDVNCGEYHT
Sequences:
>Translated_260_residues MTGMLKRKVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVAVRTDITDDDDVSNLVQATLA AYGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGTLRLIQAFTPALAQSHGAIVNVNSMVIRHSQPKYGTYKMAK SVLLAMSHSLATELGEQGIRVNSVAPGYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSDLKRLPTEDEVASAILFLAS DLASGITGQTLDVNCGEYHT >Mature_259_residues TGMLKRKVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVAVRTDITDDDDVSNLVQATLAA YGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGTLRLIQAFTPALAQSHGAIVNVNSMVIRHSQPKYGTYKMAKS VLLAMSHSLATELGEQGIRVNSVAPGYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSDLKRLPTEDEVASAILFLASD LASGITGQTLDVNCGEYHT
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI19923817, Length=275, Percent_Identity=28.7272727272727, Blast_Score=109, Evalue=3e-24, Organism=Homo sapiens, GI10190704, Length=263, Percent_Identity=30.7984790874525, Blast_Score=102, Evalue=4e-22, Organism=Homo sapiens, GI5031737, Length=255, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI59889578, Length=199, Percent_Identity=32.1608040201005, Blast_Score=92, Evalue=5e-19, Organism=Homo sapiens, GI32483357, Length=262, Percent_Identity=26.3358778625954, Blast_Score=91, Evalue=1e-18, Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=30.4568527918782, Blast_Score=87, Evalue=2e-17, Organism=Homo sapiens, GI7706318, Length=218, Percent_Identity=28.8990825688073, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=7e-14, Organism=Homo sapiens, GI126723750, Length=265, Percent_Identity=25.2830188679245, Blast_Score=72, Evalue=4e-13, Organism=Homo sapiens, GI4503301, Length=232, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI7705925, Length=260, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI126723191, Length=181, Percent_Identity=25.414364640884, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI157739938, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI20149619, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=2e-11, Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=30.46875, Blast_Score=105, Evalue=3e-24, Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=31.6205533596838, Blast_Score=100, Evalue=7e-23, Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=29.7297297297297, Blast_Score=95, Evalue=4e-21, Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=28.740157480315, Blast_Score=82, Evalue=2e-17, Organism=Escherichia coli, GI2367175, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787526, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=23.6220472440945, Blast_Score=71, Evalue=9e-14, Organism=Escherichia coli, GI1788459, Length=253, Percent_Identity=26.8774703557312, Blast_Score=68, Evalue=5e-13, Organism=Escherichia coli, GI1787820, Length=249, Percent_Identity=23.2931726907631, Blast_Score=67, Evalue=9e-13, Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=9e-12, Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=29.4339622641509, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=29.296875, Blast_Score=98, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=28.1368821292776, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17551412, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI71994604, Length=251, Percent_Identity=28.2868525896414, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI17562990, Length=257, Percent_Identity=29.5719844357977, Blast_Score=94, Evalue=9e-20, Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=29.6992481203008, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI193204405, Length=260, Percent_Identity=27.3076923076923, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17560150, Length=253, Percent_Identity=27.6679841897233, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=28.6764705882353, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=28.6259541984733, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=29.8039215686275, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=27.34375, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=28.7313432835821, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17565030, Length=262, Percent_Identity=29.3893129770992, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17536025, Length=271, Percent_Identity=28.4132841328413, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=29.5454545454545, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17562908, Length=279, Percent_Identity=27.9569892473118, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI17538486, Length=189, Percent_Identity=31.2169312169312, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=26.7924528301887, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=27.1698113207547, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17536651, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=27.3809523809524, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI25147288, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI71997402, Length=189, Percent_Identity=30.1587301587302, Blast_Score=73, Evalue=2e-13, Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=26.8939393939394, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17508651, Length=251, Percent_Identity=30.6772908366534, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17538182, Length=193, Percent_Identity=26.4248704663212, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI115534660, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17570069, Length=189, Percent_Identity=29.1005291005291, Blast_Score=68, Evalue=6e-12, Organism=Caenorhabditis elegans, GI17508895, Length=189, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI17563726, Length=193, Percent_Identity=27.4611398963731, Blast_Score=66, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=262, Percent_Identity=26.3358778625954, Blast_Score=76, Evalue=4e-15, Organism=Saccharomyces cerevisiae, GI6323882, Length=192, Percent_Identity=28.6458333333333, Blast_Score=70, Evalue=3e-13, Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=32.5490196078431, Blast_Score=115, Evalue=3e-26, Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=32.5490196078431, Blast_Score=115, Evalue=4e-26, Organism=Drosophila melanogaster, GI23397609, Length=262, Percent_Identity=33.206106870229, Blast_Score=108, Evalue=5e-24, Organism=Drosophila melanogaster, GI24644339, Length=252, Percent_Identity=31.3492063492063, Blast_Score=98, Evalue=5e-21, Organism=Drosophila melanogaster, GI24644337, Length=228, Percent_Identity=32.0175438596491, Blast_Score=95, Evalue=6e-20, Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=26.3779527559055, Blast_Score=93, Evalue=2e-19, Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=26.0700389105058, Blast_Score=85, Evalue=5e-17, Organism=Drosophila melanogaster, GI21358495, Length=190, Percent_Identity=27.8947368421053, Blast_Score=82, Evalue=3e-16, Organism=Drosophila melanogaster, GI17737361, Length=250, Percent_Identity=26.4, Blast_Score=81, Evalue=8e-16, Organism=Drosophila melanogaster, GI21357673, Length=197, Percent_Identity=28.4263959390863, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI24580925, Length=223, Percent_Identity=27.8026905829596, Blast_Score=65, Evalue=5e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 27758; Mature: 27627
Theoretical pI: Translated: 6.89; Mature: 6.89
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTGMLKRKVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVA CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEE VRTDITDDDDVSNLVQATLAAYGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGT EEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH LRLIQAFTPALAQSHGAIVNVNSMVIRHSQPKYGTYKMAKSVLLAMSHSLATELGEQGIR HHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE VNSVAPGYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSDLKRLPTEDEVASAILFLAS ECCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH DLASGITGQTLDVNCGEYHT HHHCCCCCCEEECCCCCCCC >Mature Secondary Structure TGMLKRKVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVA CCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEE VRTDITDDDDVSNLVQATLAAYGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGT EEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH LRLIQAFTPALAQSHGAIVNVNSMVIRHSQPKYGTYKMAKSVLLAMSHSLATELGEQGIR HHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE VNSVAPGYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSDLKRLPTEDEVASAILFLAS ECCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH DLASGITGQTLDVNCGEYHT HHHCCCCCCEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8868443 [H]