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The map label for this gene is fadD19 [H]

Identifier: 148663378

GI number: 148663378

Start: 3959490

End: 3961136

Strand: Reverse

Name: fadD19 [H]

Synonym: MRA_3554

Alternate gene names: 148663378

Gene position: 3961136-3959490 (Counterclockwise)

Preceding gene: 148663381

Following gene: 148663376

Centisome position: 89.62

GC content: 64.36

Gene sequence:

>1647_bases
GTGGCCGTGGCCCTGAATATTGCCGATCTCGCCGAGCACGCCATCGACGCCGTGCCTGACCGTGTCGCCGTTATCTGCGG
CGATGAGCAGTTGACCTACGCCCAGCTGGAGGATAAGGCCAACCGCCTCGCGCACCACCTGATCGATCAAGGCGTGCAAA
AGGACGACAAGGTCGGCCTGTACTGCCGCAACCGCATCGAGATCGTGATCGCGATGCTGGGCATCGTGAAGGCGGGCGCC
ATCTTGGTGAACGTCAACTTCCGTTACGTGGAGGGCGAACTTCGCTACCTGTTCGACAACTCCGACATGGTCGCGTTGGT
GCACGAACGCCGCTACGCCGACCGGGTCGCCAACGTTCTCCCCGACACGCCCCATGTAAGGACGATCTTGGTCGTCGAGG
ACGGCTCGGACCAGGACTATCGGCGCTACGGCGGCGTCGAGTTCTATTCCGCGATCGCGGCGGGCTCGCCGGAGCGTGAC
TTCGGCGAACGCAGCGCCGACGCCATCTATCTGCTCTACACCGGCGGCACCACCGGTTTCCCCAAGGGTGTGATGTGGCG
TCACGAGGACATCTATCGTGTGCTGTTCGGTGGAACCGACTTTGCGACAGGAGAGTTCGTCAAAGACGAATACGACCTGG
CCAAGGCGGCCGCGGCGAATCCACCGATGATCCGCTACCCGATCCCGCCGATGATCCACGGCGCCACCCAGTCGGCCACC
TGGATGGCGCTCTTCTCGGGCCAAACCACGGTACTGGCACCGGAATTCAACGCCGACGAGGTGTGGCGCACGATCCACAA
ACACAAGGTGAACCTGCTGTTCTTCACCGGTGATGCGATGGCCCGCCCGCTGGTCGACGCGCTGGTCAAGGGCAACGACT
ACGACCTGTCGTCGTTGTTCCTGTTGGCCAGCACCGCGGCGCTGTTCTCGCCGAGCATCAAGGAGAAACTCCTTGAGCTG
CTGCCGAATCGGGTGATCACGGACTCAATTGGCTCGTCGGAGACGGGTTTTGGCGGTACCAGCGTCGTTGCTGCCGGGCA
GGCGCATGGCGGCGGGCCCCGGGTGCGGATCGACCATCGCACCGTCGTGCTCGATGACGACGGCAACGAAGTCAAGCCCG
GCTCGGGGATGCGGGGCGTCATCGCCAAGAAGGGCAACATTCCCGTCGGCTACTACAAGGACGAGAAGAAGACGGCCGAG
ACGTTCCGGACGATTAACGGTGTGCGCTACGCCATTCCGGGGGACTACGCCCAAGTCGAGGAGGACGGCACGGTCACCAT
GCTGGGTCGTGGCTCGGTGTCGATCAACAGCGGCGGCGAGAAGGTCTACCCCGAAGAGGTCGAGGCCGCTTTGAAGGGGC
ATCCCGACGTGTTCGACGCTCTGGTGGTCGGGGTGCCCGATCCGCGTTACGGCCAGCAGGTGGCCGCCGTGGTGCAGGCC
CGGCCGGGTTGTCGGCCGTCGTTGGCCGAGTTGGACTCGTTCGTGCGCTCCGAGATCGCGGGCTACAAAGTGCCGCGCAG
TCTGTGGTTTGTCGACGAGGTGAAGCGTTCGCCCGCCGGTAAGCCGGACTACCGCTGGGCCAAGGAGCAGACCGAGGCGC
GGCCAGCCGATGACGTGCATGCGGGACACGTGACGAGCGGTGGGTGA

Upstream 100 bases:

>100_bases
CACCAGCGCGTCGGGTCCGGATTCCACGGTTGCCACGGCCGCCACCTCTTTCTGCCCGTCGGGTGTGAGCTTGTCAAGAA
ATGTAACACGTTCTAGTTTG

Downstream 100 bases:

>100_bases
TGGGTGCGGCTGCGACCGGCTCCGGACACCCCGCACGGAAGGTCGACATGGGTCGGCTGTCGTGCCTCTCGGGGGCCGAG
CCGACATCGATGTGGATGGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAVALNIADLAEHAIDAVPDRVAVICGDEQLTYAQLEDKANRLAHHLIDQGVQKDDKVGLYCRNRIEIVIAMLGIVKAGA
ILVNVNFRYVEGELRYLFDNSDMVALVHERRYADRVANVLPDTPHVRTILVVEDGSDQDYRRYGGVEFYSAIAAGSPERD
FGERSADAIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAANPPMIRYPIPPMIHGATQSAT
WMALFSGQTTVLAPEFNADEVWRTIHKHKVNLLFFTGDAMARPLVDALVKGNDYDLSSLFLLASTAALFSPSIKEKLLEL
LPNRVITDSIGSSETGFGGTSVVAAGQAHGGGPRVRIDHRTVVLDDDGNEVKPGSGMRGVIAKKGNIPVGYYKDEKKTAE
TFRTINGVRYAIPGDYAQVEEDGTVTMLGRGSVSINSGGEKVYPEEVEAALKGHPDVFDALVVGVPDPRYGQQVAAVVQA
RPGCRPSLAELDSFVRSEIAGYKVPRSLWFVDEVKRSPAGKPDYRWAKEQTEARPADDVHAGHVTSGG

Sequences:

>Translated_548_residues
MAVALNIADLAEHAIDAVPDRVAVICGDEQLTYAQLEDKANRLAHHLIDQGVQKDDKVGLYCRNRIEIVIAMLGIVKAGA
ILVNVNFRYVEGELRYLFDNSDMVALVHERRYADRVANVLPDTPHVRTILVVEDGSDQDYRRYGGVEFYSAIAAGSPERD
FGERSADAIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAANPPMIRYPIPPMIHGATQSAT
WMALFSGQTTVLAPEFNADEVWRTIHKHKVNLLFFTGDAMARPLVDALVKGNDYDLSSLFLLASTAALFSPSIKEKLLEL
LPNRVITDSIGSSETGFGGTSVVAAGQAHGGGPRVRIDHRTVVLDDDGNEVKPGSGMRGVIAKKGNIPVGYYKDEKKTAE
TFRTINGVRYAIPGDYAQVEEDGTVTMLGRGSVSINSGGEKVYPEEVEAALKGHPDVFDALVVGVPDPRYGQQVAAVVQA
RPGCRPSLAELDSFVRSEIAGYKVPRSLWFVDEVKRSPAGKPDYRWAKEQTEARPADDVHAGHVTSGG
>Mature_547_residues
AVALNIADLAEHAIDAVPDRVAVICGDEQLTYAQLEDKANRLAHHLIDQGVQKDDKVGLYCRNRIEIVIAMLGIVKAGAI
LVNVNFRYVEGELRYLFDNSDMVALVHERRYADRVANVLPDTPHVRTILVVEDGSDQDYRRYGGVEFYSAIAAGSPERDF
GERSADAIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAANPPMIRYPIPPMIHGATQSATW
MALFSGQTTVLAPEFNADEVWRTIHKHKVNLLFFTGDAMARPLVDALVKGNDYDLSSLFLLASTAALFSPSIKEKLLELL
PNRVITDSIGSSETGFGGTSVVAAGQAHGGGPRVRIDHRTVVLDDDGNEVKPGSGMRGVIAKKGNIPVGYYKDEKKTAET
FRTINGVRYAIPGDYAQVEEDGTVTMLGRGSVSINSGGEKVYPEEVEAALKGHPDVFDALVVGVPDPRYGQQVAAVVQAR
PGCRPSLAELDSFVRSEIAGYKVPRSLWFVDEVKRSPAGKPDYRWAKEQTEARPADDVHAGHVTSGG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=568, Percent_Identity=24.1197183098592, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI187761345, Length=560, Percent_Identity=25.7142857142857, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI187761343, Length=560, Percent_Identity=25.7142857142857, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=24.0234375, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI38505220, Length=490, Percent_Identity=23.0612244897959, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI58082049, Length=483, Percent_Identity=24.6376811594203, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI42544132, Length=523, Percent_Identity=23.1357552581262, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=25.0883392226148, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI4758332, Length=489, Percent_Identity=22.9038854805726, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI12669909, Length=489, Percent_Identity=22.9038854805726, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI157311624, Length=168, Percent_Identity=35.1190476190476, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI157311622, Length=168, Percent_Identity=35.1190476190476, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI122937307, Length=168, Percent_Identity=32.7380952380952, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=566, Percent_Identity=25.6183745583039, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=25.0936329588015, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=25.8652094717668, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI145693145, Length=560, Percent_Identity=23.5714285714286, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI221142682, Length=527, Percent_Identity=25.0474383301708, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=589, Percent_Identity=24.1086587436333, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=26.3736263736264, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=26.3736263736264, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=26.3736263736264, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=520, Percent_Identity=24.8076923076923, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=540, Percent_Identity=27.037037037037, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=525, Percent_Identity=24, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=22.9007633587786, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=539, Percent_Identity=24.6753246753247, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=536, Percent_Identity=23.5074626865672, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=364, Percent_Identity=25, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115534440, Length=547, Percent_Identity=22.4862888482633, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17551278, Length=602, Percent_Identity=21.2624584717608, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=541, Percent_Identity=25.3234750462107, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71983001, Length=541, Percent_Identity=25.3234750462107, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=543, Percent_Identity=23.7569060773481, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=549, Percent_Identity=26.0473588342441, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=25.9325044404973, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581924, Length=533, Percent_Identity=24.577861163227, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI18859661, Length=530, Percent_Identity=26.4150943396226, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355181, Length=507, Percent_Identity=24.2603550295858, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45550496, Length=551, Percent_Identity=22.6860254083485, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552801, Length=551, Percent_Identity=22.6860254083485, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583465, Length=496, Percent_Identity=24.1935483870968, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583463, Length=496, Percent_Identity=24.1935483870968, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281364798, Length=496, Percent_Identity=24.1935483870968, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59741; Mature: 59610

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVALNIADLAEHAIDAVPDRVAVICGDEQLTYAQLEDKANRLAHHLIDQGVQKDDKVGL
CEEEEEHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YCRNRIEIVIAMLGIVKAGAILVNVNFRYVEGELRYLFDNSDMVALVHERRYADRVANVL
EEHHHHHHHHHHHHHHHCCEEEEEEEEEEEECEEEEEECCCCEEEEEHHHHHHHHHHHHC
PDTPHVRTILVVEDGSDQDYRRYGGVEFYSAIAAGSPERDFGERSADAIYLLYTGGTTGF
CCCCCEEEEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
PKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAANPPMIRYPIPPMIHGATQSAT
CCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCE
WMALFSGQTTVLAPEFNADEVWRTIHKHKVNLLFFTGDAMARPLVDALVKGNDYDLSSLF
EEEEECCCEEEEECCCCHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
LLASTAALFSPSIKEKLLELLPNRVITDSIGSSETGFGGTSVVAAGQAHGGGPRVRIDHR
HHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECE
TVVLDDDGNEVKPGSGMRGVIAKKGNIPVGYYKDEKKTAETFRTINGVRYAIPGDYAQVE
EEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHCCEEEECCCCHHEEC
EDGTVTMLGRGSVSINSGGEKVYPEEVEAALKGHPDVFDALVVGVPDPRYGQQVAAVVQA
CCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHHHHC
RPGCRPSLAELDSFVRSEIAGYKVPRSLWFVDEVKRSPAGKPDYRWAKEQTEARPADDVH
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
AGHVTSGG
CCCCCCCC
>Mature Secondary Structure 
AVALNIADLAEHAIDAVPDRVAVICGDEQLTYAQLEDKANRLAHHLIDQGVQKDDKVGL
EEEEEHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YCRNRIEIVIAMLGIVKAGAILVNVNFRYVEGELRYLFDNSDMVALVHERRYADRVANVL
EEHHHHHHHHHHHHHHHCCEEEEEEEEEEEECEEEEEECCCCEEEEEHHHHHHHHHHHHC
PDTPHVRTILVVEDGSDQDYRRYGGVEFYSAIAAGSPERDFGERSADAIYLLYTGGTTGF
CCCCCEEEEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCC
PKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAANPPMIRYPIPPMIHGATQSAT
CCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCE
WMALFSGQTTVLAPEFNADEVWRTIHKHKVNLLFFTGDAMARPLVDALVKGNDYDLSSLF
EEEEECCCEEEEECCCCHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
LLASTAALFSPSIKEKLLELLPNRVITDSIGSSETGFGGTSVVAAGQAHGGGPRVRIDHR
HHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECE
TVVLDDDGNEVKPGSGMRGVIAKKGNIPVGYYKDEKKTAETFRTINGVRYAIPGDYAQVE
EEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHCCEEEECCCCHHEEC
EDGTVTMLGRGSVSINSGGEKVYPEEVEAALKGHPDVFDALVVGVPDPRYGQQVAAVVQA
CCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHHHHC
RPGCRPSLAELDSFVRSEIAGYKVPRSLWFVDEVKRSPAGKPDYRWAKEQTEARPADDVH
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
AGHVTSGG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]