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The map label for this gene is ilvX

Identifier: 148663373

GI number: 148663373

Start: 3946368

End: 3947915

Strand: Reverse

Name: ilvX

Synonym: MRA_3549

Alternate gene names: 148663373

Gene position: 3947915-3946368 (Counterclockwise)

Preceding gene: 148663374

Following gene: 148663367

Centisome position: 89.32

GC content: 68.35

Gene sequence:

>1548_bases
GTGAACGGTGCTCAGGCCCTGATCAACACCCTGGTCGACGGGGGTGTCGATGTGTGCTTCGCCAACCCTGGCACGTCGGA
AATGCATTTCGTGGCGGCGCTAGACGCCGTCCCGCGCATGCGTGGCATGCTGACCCTGTTCGAAGGCGTGGCTACCGGGG
CCGCCGACGGTTATGCCCGCATTGCTGGCCGGCCGGCGGCGGTGCTGCTGCATCTGGGCCCCGGACTGGGCAACGGCCTG
GCCAACCTGCACAACGCGCGCCGTGCCCGGGTGCCGATGGTGGTGGTTGTCGGAGACCACGCGACTTACCACAAGAAGTA
CGACGCCCCGCTGGAATCCGATATCGATGCGGTCGCTGGCACCGTTTCGGGATGGGTGCGCCGGACGGAGGCTGCCGCCG
ACGTCGGGGCCGACGCTGAAGCGGCCATTGCGGCCAGCCGGTCAGGTTCACAGATCGCGACGCTGATTCTGCCCGCGGAT
GTGTGTTGGAGCGACGGTGCCCACGCGGCCGCGGGCGTGCCGGCTCAGGCGGCGGCGGCGCCAGTGGACGTGGGGCCGGT
GGCTGGGGTGCTGCGCTCAGGGGAACCCGCCATGATGCTGATCGGTGGGGATGCCACCCGCGGGCCGGGGCTGACCGCGG
CTGCGCGGATCGTGCAGGCAACCGGGGCCCGTTGGTTATGCGAGACGTTTCCGACGTGCCTGGAACGTGGCGCCGGTATC
CCCGCCGTCGAACGGCTGGCCTATTTCGCTGAAGGTGCCGCGGCCCAGCTGGACGGGGTCAAACATCTCGTGCTGGCCGG
GGCCAGGTCGCCGGTGTCGTTTTTCGCCTACCCGGGTATGCCCAGTGACCTGGTGCCGGCCGGTTGTGAGGTGCACGTGC
TTGCCGAACCCGGTGGTGCGGCCGACGCACTGGCCGCGTTGGCCGACGAGGTAGCGCCCGGGACGGTTGCCCCGGTGGCG
GGCGCGTCACGTCCGCAGCTGCCGACGGGTGACCTGACGTCGGTGTCGGCGGCCGATGTGGTCGGTGCGTTGCTGCCGGA
ACGGGCGATTGTTGTCGACGAGTCGAATACTTGTGGTGTCCTGCTGCCGCAGGCCACCGCCGGGGCCCCGGCCCATGACT
GGCTTACGCTGACCGGCGGGGCAATCGGTTACGGCATCCCGGCTGCGGTCGGTGCCGCGGTCGCCGCGCCCGACCGCCCG
GTGTTGTGCCTGGAATCCGACGGGTCGGCAATGTACACGATTTCAGGATTGTGGAGCCAAGCGCGGGAGAACCTCGACGT
CACCACCGTCATCTACAACAACGGCGCCTACGACATTCTGCGGATCGAGCTGCAACGTGTCGGCGCGGGGTCGGATCCTG
GCCCGAAGGCCCTGGATCTCCTTGATATATCGCGTCCCACAATGGATTTCGTCAAGATCGCCGAAGGTATGGGTGTTCCT
GCACGTCGTGTCACCACCTGCGAGGAATTCGCCGACGCCCTGCGCGCGGCCTTCGCCGAGCCCGGGCCGCATCTGATCGA
TGTAGTGGTGCCATCGCTTGTGGGGTAG

Upstream 100 bases:

>100_bases
GTCTTGACGAACTGGGGCTAAGCGACGAAGCTCGTCGCGACTTTCTGCACGGCAACGCTGTGCGGGTATTCAAGCTGGAC
CCACGAGGAAAGGTGCAGAC

Downstream 100 bases:

>100_bases
TGCTCCCCCGTCGTGCATAAGCCCGCGAGGACAGCTGGTTGTGCTGGGGATGCGATGGCCCGGGTGTTATCGGTCGGACG
GCCATCGCCAGCCTGTGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARIAGRPAAVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGTVSGWVRRTEAAADVGADAEAAIAASRSGSQIATLILPAD
VCWSDGAHAAAGVPAQAAAAPVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERGAGI
PAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEPGGAADALAALADEVAPGTVAPVA
GASRPQLPTGDLTSVSAADVVGALLPERAIVVDESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRP
VLCLESDGSAMYTISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIAEGMGVP
ARRVTTCEEFADALRAAFAEPGPHLIDVVVPSLVG

Sequences:

>Translated_515_residues
MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARIAGRPAAVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGTVSGWVRRTEAAADVGADAEAAIAASRSGSQIATLILPAD
VCWSDGAHAAAGVPAQAAAAPVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERGAGI
PAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEPGGAADALAALADEVAPGTVAPVA
GASRPQLPTGDLTSVSAADVVGALLPERAIVVDESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRP
VLCLESDGSAMYTISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIAEGMGVP
ARRVTTCEEFADALRAAFAEPGPHLIDVVVPSLVG
>Mature_515_residues
MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARIAGRPAAVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGTVSGWVRRTEAAADVGADAEAAIAASRSGSQIATLILPAD
VCWSDGAHAAAGVPAQAAAAPVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERGAGI
PAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEPGGAADALAALADEVAPGTVAPVA
GASRPQLPTGDLTSVSAADVVGALLPERAIVVDESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRP
VLCLESDGSAMYTISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIAEGMGVP
ARRVTTCEEFADALRAAFAEPGPHLIDVVVPSLVG

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Escherichia coli, GI1790104, Length=185, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081685, Length=164, Percent_Identity=31.7073170731707, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVX_MYCTU (O53554)

Other databases:

- EMBL:   BX842583
- PIR:   G70806
- RefSeq:   NP_218026.1
- HSSP:   P07342
- ProteinModelPortal:   O53554
- EnsemblBacteria:   EBMYCT00000003275
- GeneID:   888267
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv3509c
- TubercuList:   Rv3509c
- GeneTree:   EBGT00050000014643
- HOGENOM:   HBG294482
- OMA:   YLAQPHE
- ProtClustDB:   PRK07586
- InterPro:   IPR012001
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 52073; Mature: 52073

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: BINDING 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYAR
CCHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IAGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAG
HCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
TVSGWVRRTEAAADVGADAEAAIAASRSGSQIATLILPADVCWSDGAHAAAGVPAQAAAA
HHHHHHHHHHHHHHCCCCCHHHEEECCCCCEEEEEEEECHHCCCCCCCCCCCCCHHHHCC
PVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERGAGI
CCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEPGGA
CHHHHHHHHHCCCHHHHCCHHEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCH
ADALAALADEVAPGTVAPVAGASRPQLPTGDLTSVSAADVVGALLPERAIVVDESNTCGV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEE
LLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAMYTISGLWSQ
EEECCCCCCCCCCEEEEECCCHHCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHH
ARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIAEGMGVP
HHCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
ARRVTTCEEFADALRAAFAEPGPHLIDVVVPSLVG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYAR
CCHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IAGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAG
HCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
TVSGWVRRTEAAADVGADAEAAIAASRSGSQIATLILPADVCWSDGAHAAAGVPAQAAAA
HHHHHHHHHHHHHHCCCCCHHHEEECCCCCEEEEEEEECHHCCCCCCCCCCCCCHHHHCC
PVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERGAGI
CCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEPGGA
CHHHHHHHHHCCCHHHHCCHHEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCH
ADALAALADEVAPGTVAPVAGASRPQLPTGDLTSVSAADVVGALLPERAIVVDESNTCGV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEE
LLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAMYTISGLWSQ
EEECCCCCCCCCCEEEEECCCHHCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHH
ARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIAEGMGVP
HHCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
ARRVTTCEEFADALRAAFAEPGPHLIDVVVPSLVG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230