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The map label for this gene is lipF [H]

Identifier: 148663351

GI number: 148663351

Start: 3914851

End: 3915684

Strand: Reverse

Name: lipF [H]

Synonym: MRA_3527

Alternate gene names: 148663351

Gene position: 3915684-3914851 (Counterclockwise)

Preceding gene: 148663356

Following gene: 148663349

Centisome position: 88.59

GC content: 64.87

Gene sequence:

>834_bases
GTGCGTGCGCCTGGGGTGCGGGCGGCCGACGGCGCTGGGCGGGTGGTGCTCTATTTGCACGGCGGCGCGTTTGTCATGTG
CGGGCCGAATTCGCACAGCAGGATCGTCAACGCGCTGTCAGGATTTGCCGAATCACCCGTGTTGATAGTCGACTATCGGC
TGATCCCTAAGCATTCGCTGGGGATGGCGCTCGACGACTGCCACGATGCATACCAATGGTTGCGGGCGCGCGGATACCGG
CCGGAACAGATCGTGTTGGCCGGTGATTCCGCGGGTGGTTACCTTGCGCTCGCGCTTGCGCAGCGGCTGCAGTGCGACGA
CGAGAAACCGGCGGCAATAGTGGCCATCTCTCCGTTGCTGCAGCTGGCCAAGGGGCCCAAGCAGGACCATCCCAACATCG
GGACCGACGCGATGTTTCCGGCGCGGGCATTCGATGCCTTGGCGGCATGGGTCAGGGCGGCCGCTGCCAAGAACATGGTC
GACGGCAGGCCCGAAGACCTCTATGAGCCGCTCGACCATATCGAATCCAGCTTGCCGCCGACACTTATTCACGTTTCCGG
CTCCGAAGTGCTGCTGCACGACGCGCAGCTGGGCGCCGGCAAGCTGGCGGCGGCAGGCGTCTGTGCCGAGGTACGGGTTT
GGCCCGGTCAGGCTCATCTTTTCCAACTCGCCACACCGCTGGTGCCCGAAGCGACCCGTTCGTTGCGTCAGATCGGGCAG
TTCATTCGCGACGCCACCGCTGACTCGTCGTTGAGTCCGGTGCATCGGTCGCGCTACGTCGCGGGTTCACCTCGCGCCGC
AAGCCGTGGCGCGTTTGGACAGTCGCCTATCTAG

Upstream 100 bases:

>100_bases
TCTGCCGTTGGGGTTCGTCGACTTCGCATGCAGGGTTTGGCGCCCCGGGCAGGGCACGGTCCGAACAACGATAAATTTGC
CGAATGCTACCGCCCAGTTG

Downstream 100 bases:

>100_bases
TGACCACCGGCCGGGGCCGGACCACAGCAGGAAGATCAATCCGAGCAGCATCGACAGGTCGGTCCGGGCAGCGTGGGCCA
TGCCCCAGAAGCCCTCTACC

Product: esterase LipF

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MRAPGVRAADGAGRVVLYLHGGAFVMCGPNSHSRIVNALSGFAESPVLIVDYRLIPKHSLGMALDDCHDAYQWLRARGYR
PEQIVLAGDSAGGYLALALAQRLQCDDEKPAAIVAISPLLQLAKGPKQDHPNIGTDAMFPARAFDALAAWVRAAAAKNMV
DGRPEDLYEPLDHIESSLPPTLIHVSGSEVLLHDAQLGAGKLAAAGVCAEVRVWPGQAHLFQLATPLVPEATRSLRQIGQ
FIRDATADSSLSPVHRSRYVAGSPRAASRGAFGQSPI

Sequences:

>Translated_277_residues
MRAPGVRAADGAGRVVLYLHGGAFVMCGPNSHSRIVNALSGFAESPVLIVDYRLIPKHSLGMALDDCHDAYQWLRARGYR
PEQIVLAGDSAGGYLALALAQRLQCDDEKPAAIVAISPLLQLAKGPKQDHPNIGTDAMFPARAFDALAAWVRAAAAKNMV
DGRPEDLYEPLDHIESSLPPTLIHVSGSEVLLHDAQLGAGKLAAAGVCAEVRVWPGQAHLFQLATPLVPEATRSLRQIGQ
FIRDATADSSLSPVHRSRYVAGSPRAASRGAFGQSPI
>Mature_277_residues
MRAPGVRAADGAGRVVLYLHGGAFVMCGPNSHSRIVNALSGFAESPVLIVDYRLIPKHSLGMALDDCHDAYQWLRARGYR
PEQIVLAGDSAGGYLALALAQRLQCDDEKPAAIVAISPLLQLAKGPKQDHPNIGTDAMFPARAFDALAAWVRAAAAKNMV
DGRPEDLYEPLDHIESSLPPTLIHVSGSEVLLHDAQLGAGKLAAAGVCAEVRVWPGQAHLFQLATPLVPEATRSLRQIGQ
FIRDATADSSLSPVHRSRYVAGSPRAASRGAFGQSPI

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 29430; Mature: 29430

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPGVRAADGAGRVVLYLHGGAFVMCGPNSHSRIVNALSGFAESPVLIVDYRLIPKHSL
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCC
GMALDDCHDAYQWLRARGYRPEQIVLAGDSAGGYLALALAQRLQCDDEKPAAIVAISPLL
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
QLAKGPKQDHPNIGTDAMFPARAFDALAAWVRAAAAKNMVDGRPEDLYEPLDHIESSLPP
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TLIHVSGSEVLLHDAQLGAGKLAAAGVCAEVRVWPGQAHLFQLATPLVPEATRSLRQIGQ
EEEEECCCEEEEECCCCCCCHHHHHCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHH
FIRDATADSSLSPVHRSRYVAGSPRAASRGAFGQSPI
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAPGVRAADGAGRVVLYLHGGAFVMCGPNSHSRIVNALSGFAESPVLIVDYRLIPKHSL
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCC
GMALDDCHDAYQWLRARGYRPEQIVLAGDSAGGYLALALAQRLQCDDEKPAAIVAISPLL
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
QLAKGPKQDHPNIGTDAMFPARAFDALAAWVRAAAAKNMVDGRPEDLYEPLDHIESSLPP
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TLIHVSGSEVLLHDAQLGAGKLAAAGVCAEVRVWPGQAHLFQLATPLVPEATRSLRQIGQ
EEEEECCCEEEEECCCCCCCHHHHHCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHH
FIRDATADSSLSPVHRSRYVAGSPRAASRGAFGQSPI
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]